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B Rademaker

Publications and source records attributed to B Rademaker.

26 records · Page 2Linked to original sources
PubMed1986

The effects of 4-hydroxy-2,3-trans-nonenal on beta-adrenoceptors of rat lung membranes.

Lung membranes are susceptible to oxygen radicals, formed during inflammation, redox cycling of toxic agents, exposition to ozon etc. Oxygen radicals may modify the beta-adrenergic response. However, at the same time beta-adrenoceptors of the lung are frequently addressed in therapy. We embarked upon this problem by studying the effects of the aldehyde 4-hydroxy-2,3-transnonenal (HNE), one of the major products of lipid peroxidation, on the density of beta-adrenoceptors of rat lung membranes. It is shown, that the physiological important sulfhydryl blocking agent HNE inactivates the beta-adrenoceptors in a time- and concentration dependent (0.5-2.5 mM) way, indicated by a decrease in (-)-[3H]dihydroalprenolol (DHA) binding to lung membranes. Moreover, it is shown that combined treatment of HNE with (-)-isoproterenol (0.5 microM) or 1-alprenolol (0.5-10 nM) does not influence the extent of inactivation of beta-adrenoceptors by HNE. This is in contrast with previous studies, conducted with other, synthetic, sulfhydryl blocking agents, such as N-ethylmaleimide (NEM), suggesting that an other mechanism of inactivation is involved upon HNE treatment.

Aldehydes↗
PubMed1986

Enkephalinase inhibition suppresses naloxone-induced jumping in morphine-dependent mice.

The effect of the enkephalinase inhibitor phosphoramidon on the withdrawal syndrome following acute and chronic morphine-induced physical dependence in mice, was investigated. Phosphoramidon administered intracerebroventricularly (50-200 micrograms) suppressed significantly the naloxone-precipitated withdrawal jumping and wet dog shakes while forelimb shakes were potentiated in both acute and chronic morphine-dependent mice. It is suggested that increased saturation of the opioid receptors by endogenous opioid peptides following enkephalinase inhibition, might suppress the severity of the withdrawal syndrome.

Animals↗
PubMed1985

High affinity non-beta-adrenoceptor binding of beta-adrenergic ligands.

Two types of saturable binding, besides the well-known non-specific binding, were found when hydrophobic ligands were used for investigating the vesiculization of beta-adrenoceptors on cultured HeLa and Chang liver cells. The first (compartment I) representing beta-adrenoceptors with high affinity ([3H]DHA 0.8 nM, 125I-CYP 27 pM) and low capacity (10-20 fmol/mg protein), the second (compartment II) had a rather high affinity ([3H]DHA 400 nM, 125I-CYP 30 nM) and a very high capacity (20 000-25 000 fmol/mg protein). The affinity of adrenergic agents for compartment II correlates very well (r = 0.9418) with the calculated hydrophobicity. It is concluded that these types of binding sites might interfere with the determination of adrenoceptor binding sites when hydrophobic ligands are used. When using hydrophobic ligands like these special care should be taken to avoid such interference.

Adrenergic beta-Agonists↗
PubMed1985

Non-specific binding of the fluorescent beta-adrenergic receptor probe alprenolol-NBD.

The fluorescent beta-adrenergic receptor probe alprenolol-NBD was found to exhibit a high affinity (Kd 3.2 nM) and a low capacity (10 fmol/mg protein) for the beta 2-adrenergic receptor on living Chang liver cells but also a high affinity (Kd 320 nM) for non-beta-adrenergic receptor binding sites with a very high capacity (28,000 fmol/mg protein). Calculations are presented which make clear that less than 3% of the binding of alprenolol-NBD during visualization experiments is beta-adrenergic receptor related. Furthermore, it is shown that besides the downregulation of beta-adrenergic receptors during incubation with isoproterenol, the high-affinity non-beta-receptor binding sites are also deminishing during incubation with isoproterenol. Based on our findings it is concluded that the results of Henis et al. who claimed the visualization of the beta-adrenergic receptor population on Chang liver cells by alprenolol-NBD must be interpreted as an almost completely non-specific fluorescence.

4-Chloro-7-nitrobenzofurazan↗
PubMed1976

A semiautomated method for the determination of 11-deoxy-17-oxo-steroids in urine.

A semiautomated method is described for the determination of total 11-deoxy-17-oxo-steroids (11-DOOS: androsterone, etiocholanolone plus dehydroepiandrosterone) in urine. Urinary conjugates are manually extracted on a XAD-2 resin, hydrolysed by beta-glucuronidase and "solvolysed" in acid ethyl-acetate according to Burnstein-Lieberman. The free 11-DOOS are extracted automatically with iso-octane and estimated colorimetrically by the Zimmerman reaction in an Auto-Analyzer II system. The method was evaluated by investigation of its precision, accuracy, sensitivity and specificity. It was found to be satisfactory for the rapid and reliable screening of large numbers of urine samples.

Androsterone↗