PubMed Health⌕ Search

Biomedical subjects

B Raftery

Publications and source records attributed to B Raftery.

4 recordsLinked to original sources
PubMed1986

Cell-cooling in flow cytometry by Peltier elements.

We have built a cooling device for cell suspensions in flow cytometry that makes use of the Peltier effect (Barnard RD, Thermo electricity in Metals and Alloys, Taylor and Francis, London; Siemens-Z 34:383-88, 1963). The prototype described here is used for cooling collection tubes during long-duration cell sorting and is capable of maintaining a temperature of 2-5 degrees C in a cell suspension of up to 3 ml. In general, Peltier element-based cooling is useful for equilibrating the temperature of small volumes of fluids. Furthermore, Peltier element-based cooling devices are easy to build and handle.

Animals↗
PubMed1985

A monoclonal antibody to the galactan from Helix pomatia snails recognizes beta-(1,6)-linked D-galactose residues.

Using the hybridoma technique, a series of four monoclonal antibodies to the galactan isolated from albumin glands of wine yard snails (Helix pomatia) could be generated. Characterization of the binding properties of one of these antibodies revealed a specificity for beta-(1,6)-glycosidically linked D-galactose residues. This could be demonstrated by reaction of the antibody with beta-(1,6)-D-galactans and by the D-galactose-inhibitable binding to group B streptococci of type II, which possess beta-(1,6)-linked D-galactose as immuno-determinant group.

Animals↗
PubMed1983

[Experimental studies of plasmacytoma. The idiotype as "marker"].

The idiotype is a product of malignant plasmacells and can be identified in a highly specific manner by antiidiotypic antisera. The idiotype is usefull as a tumor-specific marker in myelomas and has been worked with in different problems. The main methods used were: immunofluorescence, internal labeling of immunoglobulins with radioactive-labeled aminoacids, radio-immunobinding-inhibition assay. The results are as follows: 1. Idiotype bearing precursor-cells are detectable in the periphery of myeloma patients. But in our experimental approach we were not able to demonstrate, that these cells are in fact synthesizing the idiotype although the methode has been proved to be sensitive enough. 2. The idiotypic proportion of the total immunoglobulin-fraction (= M-component in the electrophorisis) in myelomasera ranges between 40 and 90%. This percentage remains in the order of 40% even in complete remission. 3. The idiotype as tumorspecific marker is only usefull as an early detector of relapse in patients with complete remission.

Adult↗