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Biomedical subjects

B Rassow

Publications and source records attributed to B Rassow.

16 recordsLinked to original sources

[Possible damage to the eye caused by light from ophthalmologic equipment].

Motivated by numerous findings of the damaging effect of intense light levels on the fundus we measured the spectral radiation of several ophthalmic illuminations and used it to calculate from it the spectral retinal irradiance. To evaluate the thermal hazard, we calculated the resulting temperature rise at the fundus. A measure for biological damage is the percentage of denaturated molecules, which is related to the temperature rise and its duration (essentially identical to the duration of illumination) by the so-called Arrhenius-integral. We used this measure to compare any illumination with experimentally evaluated threshold values for thermal damage. To evaluate the photochemical hazard we compared the spectral retinal irradiance with threshold values for photochemical induced lesions. Furthermore the illuminations were correlated with safety-guidelines likewise distinguishing between thermal and photochemical hazard. Under adverse conditions operation microscopes can present a thermal hazard to the patient's eye. Other ophthalmic illuminations are of less concern in this respect. A similar estimation applies to hazard from photochemical effects. The photochemical hazard can be diminished essentially by reducing the short-wavelength bluelight. Qualitatively, maximal permissible exposures according to safety guidelines are consistent with our thermal calculations as well as with photochemical hazard evaluation by comparing spectral retinal light levels with threshold irradiance for photochemical induced lesions. However, since quantitatively safety guidelines estimate the thermal hazard very conservatively, many common ophthalmic illuminations exceed the exposure limits, in some cases even drastically.

Hot Temperature

[Optical properties and clinical performance of the Topcon retinal camera TRC-FET 3 (author's transl)].

A Topcon Retinal Camera TRC-FET 3 was tested for several weeks under laboratory and clinical routine conditions. The optical properties turned out as good and meet the requirements for retinal cameras in all details. Remarkable is the high speed of the imaging system, which allows working with low flash energies and with high flash frequency. The resolving power is high (8 microns at the center) and decreases slowly to the periphery. The illumination of the fundus is fairly homogeneous. Many filters of good performance are provided. There was no problem in handling the camera, and the test photographs as well as the clinical routine photographs were of high quality.

Clinical Trials as Topic

[Laser lesions of the anterior segment of the rabbit eye. Studies of cornea, iris, lens and sclera using an argon, ruby and YAG-laser].

The anterior segment of the eye of 100 rabbits was exposed to radiation from Argon, Ruby and YAG lasers. The healing process of liminal and supraliminal lesions was evaluated histologically. The iris is the most sensitive tissue for all three lasers (threshold radiant exposure, Argon: 0.004 kJ/cm2, Ruby: 0.01 kJ/cm2, YAG: 0.05 kJ/cm2). Higher energies necessary for lesions in the transparent media were available only with the Argon and YAG lasers. The Argon laser failed to produce lesions in the lens, and threshold radiant exposure for the central cornea was 8 kJ/cm2. The thresholds for YAG lasers lesions in the central cornea and lens were of the same magnitude. The marked absorption of energy by the iris may lead to an accumulation of heat, resulting in lesions of adjacent tissues or a reduction of threshold for those lesions: The threshold in the peripheral cornea was ten times lower than in the center with the Argon laser, four times lower for the YAG laser. The thresholds for Argon and YAG laser lesions in the peripheral sclera lay between the thresholds for the iris and for the peripheral cornea. A safety standard for the anterior segment of the eye should be based on the lowest threshold (Argon laser lesion of the iris). The application of a factor 100 results in a maximal permissible exposure of 40 mJ/cm2. The influence of the beam diameter could not be evaluated.

Animals

[Electron microscopic observations on the effect of laser beams on the retina of the rabbit (author's transl)].

After laser irradations, retinas of 13 rabbits were examined by electron microscope. The laser focus was set between 30 min and 140 h or 6 weeks in advance. The anatomical separation between the retina and the connective tissue of the choriocapillary in all the lesions is preserved by an intact Bruch's membrane. Destruction of the pigment epithelium, rod layer, and outer granular layer occurs in the early stages after coagulation. Later the nuclei of the rods show various stages of degeneration with simultaneous increases in the volume of the Müller's cells. The cytoplasma of the Müller's cells penetrates the nuclei of the sensory cells undergoing degeneration. Proliferation of the pigment epithelium begins after about 20 h. The first appearance of macrophages in the retina is visible after 30 h. Between 92 and 140 h in the area of the pigment epithelium, variously differentiated cells, which partly contain pigment granules and lamellary inclusion bodies, form. Some cells or cell groups which are visible might represent histiocytes within the Bruch's membrane 35, 45, 68, and 92 h as well as 6 weeks after coagulation. They partly cross the outer layer of the Bruch's membrane and neighboring connective tissue of the choroid. According to our studies, pigment epithelial cells, choroidal histiocytes, and Müller's cells participate in the phagocytosis.

Animals

[Lesions of the rabbit cornea produced by laser light (author's transl)].

Threshold radiant exposure of the cornea of gray rabbits was evaluated for Argon- and YAG-Laser light. Threshold for the Argonlaser is about 15 kJ/cm2 (exposure time 200 ms to 2 s, spot size 65 micron). For experimental and methodical reasons the value for the YAG-Laser (115 kJ/cm2) is of limited accuracy. The healing process of supraliminal lesions of the cornea is observed clinically and histologycally up to six weeks following exposure to laser radiation.

Animals

[The prognostic value of the laser-interference-fringe-test with cataract patients (author's transl)].

The retinal visual acuity of 198 cataractous eyes was tested with interference-fringes and compared with the post-operative visual acuity. A new method of creating interference-fringes was used. The interference-fringe technique showed its special clinical value with immature cataracts. It is of certain value also with premature cataracts. With mature and hypermature cataracts no results of clinical value could be obtained. The method seems to have its value with congenital and juvenile cataracts.

Cataract

[Testing of the "retinal acuity" with the laser interference apparatus as a routine clinical method].

The retinal function may be tested by laser-interference-fringes formed on the retina independently of the refraction status of the eye. The perception of these fringes is a subjective test for the retinal function even in case of moderate opacities of the optical media. Experiences with a new apparatus described here showed that the determination of this "retinal acuity" is very simple to perform. The comparison of retinal acuity and visula acuity of several hundred cataratous eyes and the measurement before and after cataract extraction in about 100 cases are discussed. The method gives useful prognostic and diagnostic information to the ophthalmologist.

Cataract

[The astigmatism bioblique. II. Mathematical description (author's transl)].

Optical surface showing an astigmatism bioblique could only approximately be described by mathematical formulas. The best approximation is that one which shows a close relation between regular and bioblique astigmatism. It allows the calculation of the best correcting cylinder, which brings the anavoidable residual astigmatism to a minimum.

Astigmatism

[Laser trabeculopuncture. II. Screen electron mascroscopic findings].

Laser trabeculopuncture was performed on preparations of human anterior chamber angle obtained from cadaver eyes. It was possible repeatedly to make about 50 mum large openings leading from anterior chamber to Schlemm canal. It was necessary to use short exposure times of maximum 200 msec and laser efficiency of at least 1,500 mW at the origin. The results were studied with a scanning electron microscope.

Humans

[Laser trabeculopuncture. III Experiments with rhesus monkeys].

Argon-laser trabeculopuncture was carried out on 7 rhesus monkeys. Perforation of the trabecular meshwork was possible with laser impacts of 100 msec, 4,200 mW and a beam diameter of 50 mum. Clinical and histological observations covered an interval of up to 15 weeks after coagulation. They revealed an obliteration of both the trabecular holes and the canal of Schlemm after a few days. Shorter pulse durations and higher energies might be helpful to improve the effectiveness, possibly assisted by a therapy with anti-inflammatory agents.

Animals

[Laser-trabecular puncture. Experimental studies].

From the presented evidence it may be seen that a sufficiently strong laser beam can produce performation openings in trabecular meshwork. We were using an exposure time of maximum 200 msec in order not to produce thermal reactions in surrounding tissues. The 1.6 W used by us was sufficient with the above exposure time to produce repeatedly openings in trabecular meshwork. Following this procedure perfusion of Schlemm's canal experiments let the used dye pass through artificially made openings, only slightly staining the trabecular meshwork.

Iris

[Experiences with the automatic refractor "Dioptron" (author's transl)].

The consistency of values obtained by the automatic refractor "Dioptron" was evaluated by testing one subject repeatedly. The consistency was found so be significantly better than that given by subjective refraction. In about 20% of 140 eyes visual acuity found by the "Dioptron" - refraction was lower than that found by subjective refraction. Thus the results of the "Dioptron" should be controlled by a subjective refraction. The apparatus was also used in 15 children 5 to 12 years of age without problems.

Automation