PubMed Health⌕ Search

Biomedical subjects

B Rodgers

Publications and source records attributed to B Rodgers.

At least 91 records · Page 5Linked to original sources
PubMed1985

Lysis of human cytomegalovirus infected fibroblasts by natural killer cells: demonstration of an interferon-independent component requiring expression of early viral proteins and characterization of effector cells.

We investigated the susceptibility of cells infected with human cytomegalovirus (HCMV) to lysis by human natural killer (NK) cells, examining in particular its relationship to sequential viral protein expression, interferon (IFN), and the nature of the effector cells. HCMV-infected fibroblasts were lysed by peripheral blood mononuclear cells from normal seronegative individuals. The effector cells were large granular lymphocytes of Leu-7+, Leu-11+, and to a lesser extent Leu-7- phenotype. Depletion studies suggested they were the same population of NK cells that lyse uninfected fibroblasts, but a subset of NK cells that lyse K562 cells. HCMV-infected cells treated with phosphonoformate and cells infected for 16 hr that only express the nonstructural HCMV immediate early and early proteins and not the late (structural) proteins were susceptible to lysis by IFN-pretreated effector cells, whereas cells expressing immediate early antigens alone were not. This enhanced susceptibility to lysis was associated with increased effector:target binding in target cell binding assays, and was competitively inhibited by uninfected fibroblasts in cold target competition assays. It was independent of IFN release from the infected target cells or effector cells. These results suggest that the increased susceptibility to lysis by NK cells produced by a human herpes virus HCMV i) is manifest when early viral proteins are expressed, ii) is related to enhanced expression of a target structure likely to be present on uninfected fibroblasts, and iii) has a major component that is independent of IFN.

Adult↗
PubMed1982

Colposcopic patterns and their histopathologic correlation.

White epithelium, mosaicism, punctation, and atypical vessels are internationally used colposcopic terms describing an atypical transformation zone. Other morphologic findings are surface contour, clarity of demarcation, and tone of whiteness with or without atypical vessels. In the State University of New York at Buffalo affiliated hospitals, 665 cervical and vaginal atypical colposcopic patterns of 552 patients were matched with their histologic findings. Mosaicism, punctation, and white epithelium correlated with the histologic diagnosis of vaginal intraepithelial neoplasia and cervical intraepithelial neoplasia in 43%, 55%, and 42% of the cases, respectively. When white epithelium accompanied the mosaicism or punctation, the incidence of histologically confirmed vaginal and cervical intraepithelial neoplasia raised to 66% and 68%, respectively. In patients with prenatal DES exposure, mosaicism rarely (17%) correlated with the histologic findings of vaginal and cervical intraepithelial neoplasia. Overall, there was a great variability in the correlation depending on the specific colposcopic abnormalities.

Cervix Uteri↗
PubMed1981

Interaction of influenza virus with mouse macrophages.

Mouse peritoneal and alveolar macrophages differed substantially in their response to influenza in vitro. Immunofluorescent and infectious-center techniques showed that viral proteins were produced in only a small subpopulation (17%) of peritoneal macrophages and that these infected cells were removed from culture by 3 days postinfection. In contrast, alveolar macrophages were highly susceptible to influenza, and viral antigens were produced in all cells. This was accompanied by a cytopathic effect and cell death. However, no infectious virus was released and the infection was considered abortive. With mouse cytomegalovirus, however, both alveolar and peritoneal macrophages were equally restrictive, and viral antigens were produced in only 1 to 5% of either cell population. No significant differences were observed between mouse-virulent and -avirulent strains of influenza in their interaction with macrophages either in vitro or in vivo. In vivo, both strains induced an influx of cells to the alveolar spaces by 3 to 4 days postinfection, and this was reflected by a 5- to 10-fold increase in the number of "macrophages" in harvest fluids at this time. Many of these cells had an altered morphology compared with alveolar macrophages from uninfected mice, and the cell population as a whole was not susceptible to influenza. However, this resistance was lost by 7 days of in vitro culture.

Animals↗
PubMed1981

Acute behavioral effects of styrene exposure: a further analysis.

Studies were carried out on two groups to styrene-based resin. Early morning urinary mandelic acid concentrations after two days without exposure correlated with reaction time measured on arrival at work. Men were found to differ considerably in their rate of clearance of mandelic acid-those with slow reaction times. After some months at reduced exposure, a small group of men with previously high mandelic acid concentrations has speeded up on the reaction time task.

Adult↗
PubMed1980

The isolation and characterization of a clonally related series of murine retrovirus-infected mouse cells.

Starting with cloned NIH 3T3 mouse cells we have isolated a series of related lines infected with the Kirsten murine sarcoma/leukaemia (MSV/MLV) virus complex. These lines exhibit all three possible infected cell phenotopes: (i) transformed MSV/MLV producers; (ii) non-transformed MLV producers; (iii) transformed non-producers. We have also selected non-transformed revertants from one of the non-producer clones. This series of lines allows the study of the expression of the virus genome against a constant background of cellular gene expression. We have further characterized the lines with regard to anchorage dependence of growth, tumorigenicity and the presence of a rescuable sarcoma genome. The non-producer clones are uniform in their transformed properties. The revertants contain rescuable sarcoma virus, biologically indistinguishable from the original transforming virus, implying that the reversion is due to a change in cellular rather than viurs genetic information.

Animals↗
PubMed1975

Reginald C. Augustine. Apostle of optometry.

This paper concerns itself with the personal life and professional contributions of one of the great men in optometry, R. C. Augustine. This short biography includes discussions on his childhood, his introduction to the field of optometry, his early contributions in his community, his book, his achievements during the presidency of the American Optometric Association and also his extension work the following two years. In summation, a discussion of his professional philosophy will follow.

History, 19th Century↗
PubMedDate not supplied

Expression of P.69/pertactin from Bordetella pertussis in a baculovirus/insect cell expression system: protective properties of the recombinant protein.

The surface antigen P.69/pertactin of Bordetella pertussis has been expressed using the polyhedron promoter of baculovirus in cultured insect cells. Either full-length or truncated prn DNA was used to express P.69 pertactin. The full-length gene gave rise to low levels of P.93 precursor protein, some of which was processed to P.69. The shortened prn expressed P.69 pertactin directly at levels up to 3.5 mg per litre. P.69 vaccinated animals were protected against aerosol challenge with virulent B. pertussis bacteria.

Animals↗
PubMedDate not supplied

Assessment of human right ventricular cast volume by CT and angiocardiography.

Angiocardiographic methods available for cardiac chamber volume measurements have been shown to be less accurate although more practical than CT for the evaluation of the LV. To explore the capability of CT for RV volume measurements, silastic casts of seven normal RV were measured by a displacement method, conventional angiocardiographic biplane methods, and CT. The displacement method used degassing beneath vacuum to remove air trapped in the casts, and the actual volumes, which varied between 62 and 188 cc, were measured by Archimedes' principle. Cast volumes measured by biplane angiogram methods displayed a varying degree of spread around the regression line, caused by the chamber's irregular shape and its variation in orientation relative to the x-ray beams. CT measurements were in all aspects significantly more accurate.

Angiocardiography↗