Biomedical subjects

B Rohrbach

Publications and source records attributed to B Rohrbach.

6 recordsLinked to original sources

PubMed1991

Comparison of the efficacy of three commercial feline leukemia virus vaccines in a natural challenge exposure.

Forty-seven kittens were exposed for 31 weeks to 12 FeLV-positive carrier cats. The carrier cats were infected with 2 laboratory strains of FeLV and at least 2 strains of street virus. Eleven nonvaccinated control kittens and 12 vaccinated kittens were allotted to 3 groups. After 31 weeks of exposure, the following kittens were persistently blood FeLV positive by ELISA and immunofluorescence antibody (IFA) testing: 7 of the 11 control kittens, 0 of 12 kittens inoculated with vaccine A, 5 of 12 kittens inoculated with vaccine B, and 6 of 12 kittens inoculated with vaccine C. Only the kittens inoculated with vaccine A were significantly (P less than 0.05) different from the control group. After 23 weeks of exposure, culture was done to identify FeLV in the bone marrow of the kittens. Feline leukemia virus was isolated from the bone marrow of 9 of 11 control kittens. Virus was isolated from the bone marrow of 5 of 12 kittens inoculated with vaccine A, 11 of 12 kittens inoculated with vaccine B, and 10 of 12 kittens inoculated with vaccine C. Of the 17 cats that had FeLV isolated only from culture of bone marrow (negative results of blood virus isolation, ELISA, and IFA testing), 13 eliminated the virus from the bone marrow by week 31 of exposure. After 31 weeks of exposure, FeLV was isolated from the bone marrow of 8 of 11 control kittens, 0 of 12 kittens inoculated with vaccine A, 7 of 12 kittens inoculated with vaccine B, and 7 of 12 kittens inoculated with vaccine C.

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PubMed1990

[New types of virus infections of domestic animals in the German Democratic Republic. 3. Seroepizootiologic and experimental studies of herpesvirus infections in sheep--the etiology of pulmonary adenomatosis].

Neutralisation tests for antibodies against ovine herpesvirus were applied to 848 sera which had been sampled from different sheep herds across the GDR. Between six and 20 percent of sheep in the herds tested exhibited neutralising antibodies, notwithstanding their pulmonary adenomatosis status. Incidence and titre distribution of antibodies against ovine herpesviruses in pulmonary adenomatosis herds were identical with those recorded from unsuspected herds. From among 21 sheep with pathomorphologically secured pulmonary adenomatosis, six animals exhibited antibody titres just as high as those recorded from responders of all herds examined. Lambs were obtained by hysterectomy and raised without mothers and were experimentally infected with Herpesvirus ovis. All of these animals responded to infection by clearly rising titres (between 1:2 and 1:32). Adenomatous pulmonary lesions were not recordable from any of them. One lamb, following experimental ovine herpesvirus infection, exhibition, exhibited subclinical interstitial pneumonia. Herpesvirus ovis has been widespread in sheep herds across the GDR. The authors' serological and experimental investigations do not support the assumption of an aetiological relationship between ovine herpesvirus infection and incidence of pulmonary adenomatosis.

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PubMed1983

[Immunomodulation with symptomatically effective antirheumatic agents].

As antigen-presenting and/or monokine-secreting cells, macrophages play a major role in immunoregulation. Proteases of macrophage origin (cathepsin G, elestase , thrypsin and pronase) act on cell surfaces of different cell lines, inducing cell activation, e.g. of B-lymphocytes. T-lymphocytes might be stimulated by the activating factor LAF. Other macrophage products (CSF, FIM ) control monocyte production in bone marrow. While lymphocytes are the target cell lines for classical immunosuppressive agents, mononuclear phagocytes are kept for the major cell population affected by antiinflammatory drugs. The presented study outlines the significance of the mononuclear-phagocyte-system in antiinflammatory drug research. The inhibiting potency of antiinflammatory drugs on the monocyte-macrophage cell line as an additional immunoregulatory principle should be discussed.

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PubMed1983

[Immunomodulation by symptomaticly active antirheumatic agents].

As antigen-presenting and/or monokine-secreting cells, macrophages play a major role in immunoregulation. Proteases of macrophage origin (cathepsin G, elestase, thrypsin and pronase) act on cell surfaces of different cell lines, inducing cell activation, e.g. of B-lymphocytes. T-lymphocytes might be stimulated by the activating factor LAF. Other macrophage products (CSF, FIM) control monocyte production in bone marrow. While lymphocytes are the target cell lines for classical immunosuppressive agents, mononuclear phagocytes are kept for the major cell population affected by antiinflammatory drugs. The presented study outlines the significance of the mononuclear-phagocytesystem in antiinflammatory drug research. The inhibiting potency of antiinflammatory drugs on the monocyte-macrophage cell line as an additional immunoregulatory principle should be discussed.

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PubMed1981

[Histological classification of rheumatoid synovitis compared with cell-kinetics and morphology of experimental immune synovitis (author's transl)].

During the course of the experimental immune arthritis of the guinea pig four phases can be distinguished: I=fibrinopurulent, partly ulcerative synovitis, II=granulocytic activee granulomatous synovitis, III=lymphocytic activ granulomatous synovitis, IV=synovitic sequelae with subintimal fibrosis. On the basis of cell-kinetic studies with initial and systemic labelling with 3H-thymidine of the synovial membrane and of the bone marrow it could be demonstrated that in this model the infiltrating cells as well as the structural cells belong to the monocyte-macrophage system. The typical multilayer of the lining cells of this immune synovitis therefore seems to be mainly as a type A cell hyperplasia of bone marrow derived cells instead of a local proliferation of the lining cells. Clinical cases of various joint diseases are described including symptoms, X-ray findings and histopathological studies. Depending on the stage of the clinical course and the number, localization and activity of the macrophages within the synovial membrane the reaction pattern can be classified in a new way using the categories mentioned above in the experimental model. Thus the classical picture of rheumatoid arthritis corresponds to the synovitis of the subintimal type. The polyarthritic syndrome which mostly is pathogenetically unclear in contrast is designated as synovitis of the intimal type. The same holds true for the activated arthrosis.

Aged↗

PubMedDate not supplied

Gradual occlusion of extrahepatic portosystemic shunts in dogs and cats using the ameroid constrictor.

Gradual occlusion of the splenic vein, using a specialized device (ameroid constrictor), was evaluated experimentally in three normal beagle dogs. Splenoportograms were used to verify that total occlusion of the splenic vein had occurred in all dogs within 4 to 5 weeks after application of the device. The ameroid constrictor (AC) was also evaluated as a method of gradual vascular occlusion in 12 dogs and two cats with single, extrahepatic, portosystemic shunts (PSS). Serum bile acid (SBA) concentrations were measured and portal scintigraphy (PS) was performed on all 14 animals preoperatively and 10, 20, 30, and 60 days postoperatively. Two dogs (14%) died from portal hypertension in the early postoperative period. One dog and one cat developed multiple acquired PSS, confirmed by mesenteric portography 90 days after the operation. Portal scintigraphy confirmed total occlusion of the primary shunt in the other 10 animals. Shunt fractions (SF), as measured by PS on postoperative days 30 and 60, declined significantly from preoperative values. Significant decreases were noted between preoperative and postoperative values for preprandial SBA on postoperative day 60 and for postprandial SBA on postoperative day 30. SBA concentrations did not correlate with SF. Based on this study, gradual vascular occlusion using the AC is recommended as a method for treatment of single, extrahepatic, PSS.

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