PubMed Health⌕ Search

Biomedical subjects

B Rolfe

Publications and source records attributed to B Rolfe.

9 recordsLinked to original sources
PubMed1990

Mixed gonococcal and mycobacterial sepsis of the wrist.

Mycobacterial infections of the hand and wrist are rare. Concurrent infection of a joint by more than one organism is also unusual. A 25-year-old man developed wrist sepsis caused by Neisseria gonorrhoeae and Mycobacterium avium intracellularis. The infection was successfully treated by wrist drainage, carpal debridement, and intravenous antibiotics. Secondary carpal reconstruction was accomplished by delayed bone grafting and internal fixation to preserve radiocarpal motion.

Adult↗
PubMed1989

Genetically-restricted effector molecules released by human lymphocytes in response to early pregnancy factor.

The binding of early pregnancy factor (EPF) to lymphocytes stimulates the release of soluble effector molecules. Studies in mice have shown that it is these factors rather than EPF as such which are inhibitory in the T cell-dependent reactions, the adoptive transfer of contact sensitivity and the rosette inhibition test. Two factors have been identified: mEPF-S1 (Mr approximately 15,000) is major histocompatibility complex (MHC)-restricted while mEPF-S2 (Mr approximately 55,000) is restricted to a locus (or loci) outside the MHC. In the present paper, evidence is presented which shows that EPF also induces the release of soluble mediators from human lymphocytes. With the rosette inhibition test two factors have been detected, both of similar size and genetic restriction to those described previously in the mouse. One factor, designated hEPF-S1 (Mr 14-18,000), is human lymphocyte antigen (HLA)-restricted and the other, hEPF-S2 (Mr 50-60,000), appears to be restricted to a locus (or loci) outside the HLA complex.

Chaperonin 10↗
PubMed1989

Assessing fibular length using bimalleolar angular measurements.

Shortening of the fibula after fracture is common and often difficult to appreciate. Loss of lateral malleolar anatomy causes significant biomechanical changes in the ankle and correlates with poor clinical results. We studied angular measurements of distal fibular length to serve as a guide for assessing fibular reduction after ankle fracture. Mortise view X-rays of 50 normal ankles from 25 healthy volunteers were obtained. The average talocrural angle measured 78.5 degrees. However, individual variation was high with values ranging from 75 to 86 degrees. Comparing contralateral ankles demonstrated an average difference of 1.3 degrees (range 0 to 4 degrees). A new, simpler bimalleolar angle was devised which compares the long axis of the fibula with a line drawn between the tips of the malleoli. The average bimalleolar angle measured 77.8 degrees (range 72 to 86 degrees). The contralateral difference averaged 1.2 degrees (range 0 to 3 degrees). This angle was simpler to use and more reproducible. Angular measurements were tolerant of usual radiographic techniques. Internal or external rotation of the ankle up to 5 degrees caused an insignificant change in the angular measurements. One degree change in the talocrural or bimalleolar angle was found to correspond with a 1 mm change in fibular length for the average ankle, calculated radiographically and confirmed in a cadaver study. Abnormal fibular shortening is detected with an angular difference between injured and contralateral sides of 3.0 degrees using the talocrural angle or 2.5 degrees using the bimalleolar angle (95% confidence limits). Thus, a 2.5 to 3.0 degrees contralateral difference should serve as a minimum value required to direct a change in therapy.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1985

[3H] diazepam binding to human granulocytes.

[3H]-diazepam binds to sites on human granulocyte membranes, with little or no binding to platelets or lymphocytes. These [3H]-diazepam binding sites are of the peripheral type, being strongly inhibited by R05-4864 (Ki = 6.23nM) but only weakly by clonazepam (Ki = 14 microM). Binding of [3H] diazepam at 0 degree is saturable, specific and stereoselective. Scatchard analysis indicates a single class of sites with Bmax of 109 +/- 17f moles per mg of protein and KD of 3.07 +/- 0.53nM. Hill plots of saturation experiments gave straight lines with a mean Hill coefficient of 1.03 +/- 0.014. Binding is time dependent and reversible and it varies linearly with granulocyte protein concentration over the range 0.025-0.300 mg of protein.

Benzodiazepines↗
PubMed1979

Galactose-1 phosphate uridyl transferase deficiency in the western grey kangaroo (Macropus fuliginosus; marsupialia): a model system for gene therapy studies.

The western grey kangaroo (Macropus fuliginosus) was found to be deficient for galactose-1 phosphate uridyl transferase (GPUT). This species could be used therefore for studies of gene therapy techniques. An improved GPUT assay procedure was developed. It was found that phage particles injected intravenously remain in the blood of kangaroos until the particles are removed by the reticuloendothelial system or, if this system is overloaded, the particles are inactivated by the immune system four days later. No effective transgenosis was detected of the bacterial gal operon contained in the phage used.

Animals↗
PubMed1977

An early pregnancy factor detected in human serum by the rosette inhibition test.

Modification of maternal lymphocyte activity has been demonstrated early in pregnancy by the rosette inhibition test. Normal human lymphocytes showed a similar depression of activity after incubation in serum from pregnant women, indicating that the response was caused by a serum factor. This early pregnancy factor has been differentiated from other substances which appear later in pregnancy and which may also be involved in the suppression of the maternal response. The results of this investigation suggest that the early pregnancy factor may be necessary for the continued viability of the early embryo.

Antilymphocyte Serum↗