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B Rolstad

Publications and source records attributed to B Rolstad.

9 recordsLinked to original sources
PubMed1978

Toxicity of Na251CrO4 when used to label rat lymphocytes.

Lymphocytes preincubated with various activities of Na251CrO4 were tested for their ability to induce a local, popliteal lymph node graft-versus-host (GVH) reaction. Incubation with 100 micronCi of the isotope per ml of cell suspension impaired the GVH activity, 25 micronCi gave a reduced response in 1 out of 3 experiments, whereas 10 micronCi had no effect. The reduced GVH response was due to radiation rather than chemical damage of the cells. Preincubation of the cells with either cold Na2CrO4 or outdated Na251CrO4 with concentrations of Cr identical to the ones used to label cells with 100 micronCi/ml cells did not impair the GVH reactivity. The cellular uptake of Na251CrO4 showed first-order kinetics over wide concentration ranges. Mitomycin C-treated lymphocytes showed a more heavily depressed GVH reactivity than would be expected from their moderately reduced ability to accumulate in the lymph nodes. It is recommended that doses of Na251CrO4 in excess of 10 micronCi/ml cells should not be used for functional studies of lymphocytes.

Animals↗
PubMed1977

The host component of the popliteal lymph node graft-versus-host (GVH) reaction. Identification of cells in the node and kinetics of cell proliferation.

The cellular composition of rat popliteal lymph nodes undergoing a GVH reaction was examined. The great majority of cells isolated from the nodes were lymphocytes, while the contribution of phagocytic cells was negligible (less than 5%). Both T and B cells were present in GVH nodes. However, the proportion of B cells was higher than in normal lymph nodes (40-50% vs 30%). Cells synthesizing DNA were enumerated autoradiographically in sections of GVH nodes up to day 7 of the response. A significant increase in the proportion of 3H thymidine labelled cells had already been found on the third day of the GVH response, before lymph node enlargement was apparent. Of the differnt days tested a maximum of 6% labelled cells was found on day 7. Liquid scintillation counting of cell suspension from such nodes showed an even higher increase in 3H thymidine incorporation in GVH vs normal nodes. These findings indicate that cell proliferation is a central mechanism behind the lymph node enlargement.

Animals↗
PubMed1977

The role of passenger lymphocytes in rat skin allografts.

Alloantibody formation (as measured by the haemagglutination assay) and graft rejection time were measured in HO rats receiving skin grafts from the Ag-B incompatible DA or (HO X DA)F1 strain. Pretreatment of the graft donor with irradiation (1 000 R) or thoracic duct drainage for 5 days, though reducing the level of circulating lymphocytes in these rats drastically, did not impair the host immune responses. Irradiation of graft donors with 1 500 rads did reduce the alloantibody response in the DA leads to HO combination, but this dose also impaired proper graft healing. Lymphocytes injected subcutaneously in small doses (0.3 and 3 X 10(6)) cells) immunized the recipients efficiently for a haemagglutinin response; however, the response developed in a much quicker tempo than the response after skin grafting. Skin grafts varying in size by a factor of approx. 4 evoked alloantibody formation of identical magnitude. On the basis of this and other recent evidence, it is suggested that the antigenicity of rat skin is not critically dependent on the presence of passenger lymphocytes in it.

Animals↗
PubMed1976

The host component of the graft-versus-host reaction. A study on the popliteal lymph node reaction in the rat.

I have measured the proportion of host cells in a popliteal lymph node undergoing a graft-versus-host (GVH) reaction. By using cytotoxic alloantisera with specificity for donor and host Ag-B determined antigens, it was found that about 90% of live cells suspended from the node were of host origin. The role of host T lymphocytes in this reaction was evaluated by investigating GVH-reactivity in hosts depleted of T cells (B rats). These rats differed very little from normal hosts with regard to lymph node weight increase (which was, in fact, slightly enhanced), increase in node cellularity, and donor/host contribution. The number of i.v. infused 51Cr-labelled thoracic duct lymphocytes localizing in popliteal lymph nodes undergoing a GVH-reaction was increased, but the significance of this phenomenon for the lymph node enlargement is questionable.

Animals↗
PubMedDate not supplied

The role of human endothelial cells in allograft rejection. Some in vitro correlates.

Endothelial cells, separated from the inside of the umbilical vein by collagenase digestion, could clearly stimulate allogeneic lymphocytes to blastogenesis and increased DNA synthesis in mixed lymphocyte endothelial-cell cultures. Many of the characteristics of these mixed cultures were similar to those found in mixed cultures consisting exclusively of allogeneic lymphoid cells; the MLC response. Endothelial cells could also be destroyed in vitro by cells sensitized in mixed lymphocyte cultures. When incubated in the presence of specific HL-A antisera, they were destroyed in non-immune peripheral blood mononuclear cells. These three reactions are thought to be in vitro correlated to some of the events taking place following allografting in vivo.

Antibodies↗