Biomedical subjects
Publications and source records attributed to B Rowan-Kelly.
Mice were immunized with 0.25 ml culture supernatant intraperitoneally and challenged with 5 x 10(4) Naegleria fowleri intranasally. Survival rate was 11% after one and 25% after three immunizing doses, compared to 0% in controls.
Explore the source record for details and available documents.
The effect of mefloquine, a new antimalarial compound, on the immune response in mice was studied in vitro and in vivo. Slight inhibition of mitogen-induced lymphocyte proliferative responses was observed at a concentration of 1 microgram/ml, and marked cytotoxicity at 4 microgram/ml. In contrast, a higher proportion of human lymphocytes remained viable at the same concentration of mefloquine. Antibody responses to sheep erythrocytes were impaired in mice receiving a total dose of 60 mg/kg. At this dosage schedule, delayed-type hypersensitivity responses to the same antigen were not affected.
The treatment of primary amoebic meningoencephalitis was examined using a mouse model. Rifamycin by itself was ineffective. However, a synergistic effect was observed when used in combination with amphotericin B. This finding may prove useful in the treatment of patients with primary amoebic meningoencephalitis, for which satisfactory treatment is currently unavailable.
The polyene antibiotic amphotericin B (AmB) caused a marked suppression of the cell-mediated immune response in mice. Similar treatment did not effect the humoral antibody response. The immunosuppressive property of the drug was related to its ability to inhibit the manifestation rather than the induction phase of the delayed-type hypersensitivity response. In vitro AmB suppressed mitogen-induced lymphocyte proliferation. The drug seemed to act at the proliferative phase of the response. Results presented show that the T cell response was much more sensitive to the action of AmB than was the B cell response. During AmB chemotherapy consideration must be given to the immunosuppressive properties of this drug.
The efficacy of tetracycline and amphotericin B in the treatment of primary amoebic meningoencephalitis was studied by means of a mouse model. The results showed marked synergism between these two drugs. Mice who recieved 50 microgram amphotericin B daily succumbed to the infection with only 28.6% survival compared to 0% in untreated controls. In contrast, survival percentage was increased to 66.7% and 84.6% in mice receiving the combined dosage schedule of 50 microgram amphotericin B with 2 tetracycline, and 50 microgram amphotericin B with 3 mg tetracycline, respectively. The finding of synergism between tetracycline and amphotericin may provide a better means for the treatment of this disease.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Naegleria fowleri, a free-living ameboflagellate, is the causative organism of primary amebic meningoencephalitis. Intranasal inoculation of N. fowleri in mice produces an infection similar to human disease. Mice immunized with live N. fowleri by intraperitoneal injection were found to be more resistant to subsequent intranasal challenge. These results may provide a lead to the development of immunotherapy for this virulent disease for which satisfactory chemotherapy is presently unavailable.
Explore the source record for details and available documents.