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Biomedical subjects

B S Naroditskiĭ

Publications and source records attributed to B S Naroditskiĭ.

At least 19 recordsLinked to original sources
PubMed2006

[The technologies of genetic engineering in treatment of chronic lower limb ischemia].

The article contains experimental data on angiogenesis stimulated by plasmid containing the angiogenin gene. After the introduction of the gene construction, the number of capillars in the chorion-allantois membrane increases 2 to 3 times; in an ischemized limb of a rat it increases by 20 to 30%. Intramuscular administration of genetic engineering construction to patients with chronic lower limb ischemia improved the patients' condition, consisting in an increase in painless walking distance and ankle-brachial index, as well as in trophic defect healing and the betterment of muscular perfusion. Positive effects were noted after 2 to 4 weeks of treatment and remained during 6 to 24 months. There were no side-effects, except low grade fever during 1 to 2 days.

Adenoviridae↗
PubMed2005

[Genetic vaccines].

Vaccination with DNA-vaccines is a new approach in immunization that implies application of genetic constructions coding certain proteins of various pathogens instead of conventional ways of vaccination with inactivated or attenuated microorganisms. The main advantage of this approach consists in possibility to induce immune response in cases when the disease cannot be prevented by routinely used prophylactic vaccines. This advantage is attributed to the ability of DNA-vaccines to focus immune response (cellular, humoral or both) upon one or several certain pathogenic antigens, which is impossible in principle when conventional vaccines are used and does not occur during an infectious process. The efficiency of DNA-vaccines is currently being tested in various pre-clinical and clinical trials. Genetic vaccination has induced protective or therapeutic immune response in a significant number of these trials. However, despite numerous positive results, clinical use of DNA-vaccines has not been recommended yet.

Animals↗
PubMed2004

[The CELO-ANG recombinant avian adenovirus with human angiogenine gene inducing neovascularization in the anterior tibial muscle of rat].

The CELO recombinant avian adenovirus carrying the gene coding the human angiogenine (ANG) synthesis was obtained. Expression of the angiogenine gene was shown in the LMH cell culture after infection with the CELO-ANG virus. The ability of CELO recombinant adenoviruses to carry out the delivery and expression of alien genes in muscle cells was demonstrated in experiments with laboratory animals (Wistar line rats). The induced neovascularization in rat muscles after the animals were administered the CELO-ANG viruses was shown.

Angiogenesis Inducing Agents↗
PubMed2003

[The induction of neovascularization in chorioallantoic membrane of chicken embryos transfected by a recombinant plasmid containing human angiogenin gene].

A method was elaborated to evaluate the biological activity of expression products of gene in the plasmid vectors, which are crucial for the synthesis of growth factor of blood vessels. It was proven as possible that the chrioallantonic membrane (CAM) of chicken's embryos could be transfected by recombinant plasmids containing both the reporter and target genes. The efficiency of CAM transfection was assessed by a plasmid carrying the reporter gene of green fluorescent protein (GFP). Finally, it was demonstrated that, at an infiltration of the recombinant plasmid containing the human angiogenine gene, its expression products induce the neovascularization in the CAM cells of chicken's embryos and stimulate an accretion in vessels of the 1st, 2nd and 3d orders.

Animals↗
PubMed2002

[Eukaryotic vectors of Celo avian adenovirus genome, carrying GFP and human IL-2 genes].

Recombinant CELO avian adenoviruses carrying green fluorescent protein (GFP) and and human interleukin-2 (IL-2) genes were obtained by homologous recombination in cell culture. The resultant recombinant CELO viruses are reproduced in chick embryos in the renal tubular and chorionic allantoic membrane cells. The ability of CELO vectors to transduce human and animal cells was studied in vitro (in cell cultures) and in vivo (in laboratory animals). GFP gene delivery and expression in recombinant CELO virus in tumors in C57BL/6 mice were for the first time demonstrated for B16 melanoma. Human IL-2 gene expression and protein accumulation in allantoic fluid of chick embryos infected with CELO-IL-2 vector were detected for the first time.

Allantois↗
PubMed2002

[Delivery of secreted placental alkaline phosphatase (SEAP) gene in vitro and in vivo as a component of recombinant avian adenovirus (CELO)].

Recombinant adenoviruses capable of expressing the gene of secreted placentary alkaline phosphatase (SEAP) under control of CMV-promoter was obtained on the basis of CELO avian adenovirus and human adenovirus-5 (Ad5) genomes. The efficiency of the CELO vector was determined in experiments with transduction of human (293, A549, and H1299), mouse (B16), and avian (LMH) cell cultures. It was shown in C57BL/6 mice in vivo that SEAP gene is expressed under conditions of intravenous, intranasal, and intratumoral application of recombinant adenovirus CELO-SEAP. The duration of expression of the alkaline phosphatase CELO = SEAP gene in immunocompetent mouse body was 21 days. The level of SEAP gene expression was measured in the allantois fluid of chicken embryo infected with recombinant adenovirus CELO-SEAP.

Alkaline Phosphatase↗
PubMed2000

[Sequence analysis of hexon gene from adenovirus KR95 inducing hydropericardium syndrome in chickens].

The nucleotide sequence of a part of the HindIII-D fragment (3300 b.p.) of adenovirus KR95 DNA has been determined. Analysis of the nucleotide sequence disclosed a continuous ORF for hexon gene (2814 b.p.) coding the 937 residue protein, part of ORF for the C-terminal region of pVI polypeptide, including 114 residues and the beginning of ORF coding 25 N-terminal residues for viral endoproteinase. Comparison of predicted KR95 hexon sequence and 8 mammalian and avian adenovirus hexon sequences revealed the highest homology between KR95 strain and avian adenoviruses FAV10 and FAV1 (91.1 and 80.1%, respectively). The results were used for creating a test system on the basis of the polymerase chain reaction. The system was used in analysis of fowl samples obtained from 12 poultry farms in Russia. The sequences of hexon gene amplified fragments in the isolated strains and similar fragments of other mammalian and avian adenoviruses have been compared.

Adenoviridae↗
PubMed1988

[Cloning of terminal fragments of the avian adenovirus CELO genome and isolation of a recombinant plasmid carrying the viral oncogene].

The terminal fragment of avian adenovirus CELO has been cloned in a plasmid vector. The obtained recombinant plasmid pCBE1 carries the terminal BamHI-E fragment of CELO DNA. Transfection of a nonpermissive culture of Rat2 cell line by the plasmid DNA results in formation of transformation focuses. The cloned BamHI-E fragment of CELO DNA is concluded to contain the viral oncogene. Thus, the CELO genome region deriving the BamHI-E fragment is "left".

Adenoviridae↗
PubMed1986

[Enhancement of the transforming activity of simian adenovirus S5 and of the DNA from human adenovirus type I by benzo(a)pyrene and TPA].

Benz(a)pyrene in a concentration of 2.5 micrograms/ml produced a 3.3-fold increase in the transforming activity of simian adenovirus S5 in primary cultures of rat kidney cells. Under analogous conditions TPA increased (2.1 times) the transforming activity of DNA of human adenovirus of type I (AdI). PX5 cells (cotransformed by S5 and benz(a)pyrene) induced tumours in 100% of syngeneic and 81% xenogeneic animals. PX4 cells (transformed by S5 only) induced tumours in 60% of syngeneic animals. Cells transformed by DNA AdI and DNA AdI + TPA did not induce tumours in animals.

Adenoviridae↗
PubMed1985

[Molecular biology of natural and constructed in vitro recombinants of human adenoviruses and SV40].

This review is devoted to the molecular and biological properties of natural and artificial Ad-SV40 hybrids. The conditions of adenovirus and SV40 recombinant formations are discussed. The principal one is the ability of the C-terminal portion of the SV40 T-antigen to carry a helper function for growing human adenoviruses in the cultures of simian cells, in which the adenoviruses themselves are not reproduced. The data about the structural and functional organization of the hybrid RNA and proteins, tumor properties of defective and nondefective Ad-SV40 recombinants are provided. A special chapter is devoted to the construction of Ad-SV40 hybrids in vitro. The possibility of using them not only for the investigation of adenoviral properties, but for the super production of different proteins is marked. Alongside the corresponding region of SV40 genome may perform the function of a selection marker for the creation of eucaryotic vectors on the basis of adenoviruses.

Adenoviruses, Human↗
PubMed1985

[Effectiveness of incorporation of spermine-condensed viral DNA into liposomes. Interaction of liposomes with cells].

DNA from bacteriophage lambda or monkey adenovirus type 7 have been condensed with spermine and included into three types of liposomes: ethereal, obtained by inverted phases technique and by Ca++ fusion. Compact DNA form presents a tor with 100 nm external diameter and 430 nm width. It can be included into 100 nm or larger liposomes. Total preparation contains 15-18% of the required liposomes. Liposomal fractions with included DNA were separated from empty liposomes by step gradient of ficoll 400. Liposomal fraction having included DNA contains 15-18% of common lipid. Liposomal interaction with monkey cell cultures has been studied.

Animals↗
PubMed1985

[The use of the cationic detergent dodecyltrimethylammonium bromide for the isolation of influenza virus glycoproteins with subsequent integration of the protein into liposome membrane].

Glycoproteins were isolated from influenza virus with the use of cationic detergent dodecyltrimethylammoniumbromide and attached to preformed liposomes. Liposomal vesicles, thus, acquired their ability for hemagglutination and lysis of chicken erythrocytes. The possibility of using these liposomes for transfer of alien agents into eucaryotic cells is discussed.

Detergents↗
PubMed1984

[Isolation and characteristics of the fibroblast cell line of rats cotransformed by the DNA of simian adenovirus SA7 (C8) and hepatitis B virus].

Transformed cell line (SH2) was established by means of co-transfection of primary rat embryonal fibroblasts by DNA of high-oncogenic simian adenovirus SA7 (C-8) and hepatitis B virus. SH2 cells possess a transformed phenotype and high oncogenicity both for allogenic (rats) and xenogenic (hamsters) animals. 100 SH2 cells induce tumours in newborn (3 day) hamsters. 10(4) SH2 cells inoculated to adult hamsters induce tumours in approximately 50% cases on the 20th-30th day. Possible mechanisms of significant stimulation of SH2 cells oncogenic properties after co-transfection by DNA of oncogenic adenovirus SA7 and hepatitis B virus are discussed.

Adenoviridae↗
PubMed1982

[Use of affinity chromatography for the purification of specific endonuclease Eco RI and Bg1 II].

The highly active preparations of specific endonucleases Eco RI and Bgl II were purified by affinity chromatography from E. coli and Bacillus globiggii cells, respectively. The isolation and purification procedures included cell disruption by ultrasonication, ultracentrifugation and chromatography. Blue dextrane-Sepharose, folate-Sepharose and phenyl-Sepharose were used as affinity adsorbents. The optimal conditions for the adsorption and elution of the endonucleases excluding intermediate steps of dialysis and concentration were selected. A high degree of purification was achieved by a consecutive use of adsorbents with different ligands. The purified enzyme does not contain non-specific nucleases or phosphatases, is sufficiently concentrated and can be used for specific hydrolysis of DNA.

Bacillus↗