Effect of deladroxone on spermatogenesis in rats and rhesus monkeys.
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Algestone Acetophenide↗
Biomedical subjects
B S Setty
Publications and source records attributed to B S Setty.
Effect of progestational steroids on thyroid function in rhesus monkeys.
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Animals↗
Studies with an intravas nylon device in rat.
Contraceptive efficacy of an intravas device (IVD) was investigated in rat. The IVD used consisted of a nylon suture which did not occlude the lumen of the vas completely and permitted a free passage of spermatozoa. Its presence for periods ranging from 15 to 180 days did not produce any changes in the histology of the testis and epididymis; In the vas deferens the tall columnar ciliated epithelium was replaced by squamous epithelium. A spermatic granuloma developed on the vas at the entry of the IVD and its frequency of occurrence and size increased with the passage of time. The device caused an increase in the vasal protein, sialic acid and phospholipid content, and a decrease in the level of glycogen and alkaline phosphatase activity at 15 days. The level of portein, glycogen and alkaline phosphatase activity returned to normalcy at 90 days but the sialic acid and phospholipid content remained elevated. Notwithstanding these alterations the device failed to affect the spermatozoal number, morphology, motility and their fertilizing ability. The significance of these findings is discussed.
Animals↗
Flutamide as an androgen antagonist on epididymal function in the rat.
The purpose of this study was to investigate whether flutamide (alpha-alpha-alpha--Trifluro-2-methyl-4'-nitro-m-propionatoluidide), a nonsteroidal compound acts as an androgen antagonist at the level of the epididymis. For comparison, cyproterone acetate was also evaluated under identical experimental conditions. The levels of glycerylphosphorylcholine (GPC), sialic acid, total phospholipids were used as functional indices of epididymis and, in addition, weights of the accessory sex organs were recorded. Adult male rats castrated and maintained on testosterone therapy (TP: 250 microgram/rat: i.m.) were treated with flutamide (25 mg/kg, oral) or cyproterone acetate (25 mg/kg, s.c.). Castration caused a reduction in the weight and secretory activity of the epididymis which was restored to near normal following TP replacement therapy. GPC level was found to be the most sensitive index of epididymal secretory function. The two antiandorgens antagonised the action of TP on all the accessory sex organs. These findings are discussed in relation to our previous study which demonstrated the lack of effect of flutamide on the epididymal function of intact rats.
Anilides↗
In vitro metabolism of (3H)-androstenedione in the rat epididymis and vas deferens.
The in vitro metabolism of (3H)-androstenedione in the epididymis and vas deferens of intact and castrated rats was investigated and the metabolites formed were identified by radio gas chromatography. Incubation of slices of caput epididymidis for 2 hr at 34 degrees C metabolised 90% androstenedione. Similar incubations of tissue samples from cauda epididymidis and vas deferens metabolized 60 and 25% of androstenedione respectively. The major metabolites formed in the epididymis were androstanedione (caput: 48%; cauda: 33%) and androsterone (caput: 35%; cauda: 13%). These metabolites appeared in much less concentration in the incubations with vas deferens (about 8% each). In general, conversion to testosterone and dihydrotesterone was low in all the three organs examined. Castration did not significantly alter the metabolic pattern in the caput epididymidis and vas deferens but promoted the formation of androsterone (38%) in the cauda epididymidis. The conversion of androstenedione, a weak androgen to testosterone, dihydrotestosterone and 3 alpha/3 beta-diols in the epididymidis and vas deferens of castrated rats may be of physiological significance. In addition, androsterone appears to be an important androgenic metabolite in the epididymis.
Androstenedione↗
Sexual maturation in male rats after centchroman and naphthofuran administration during neonatal period.
Male rats given a single s.c. injection of 250 micrograms estradiol dipropionate on day 5 postnatal life failed to show maturational changes in the testis and the growth of accessory sex organs was reduced. Similar treatment with two non-steroidal 'estrogenic' compounds viz. Centchroman (500 micrograms) and Naphthofuran (500 micrograms) retarded the growth of the testis and accessory organs but spermatogenesis was not affected. These effects persisted through 90 days. The results of the present study suggest that the two nonsteroidal compounds being 'atypical' estrogens, their effect on hormone responsive centres of brain also is less severe as compared to that caused by classical estrogen like estradiol.
Animals↗
Biological action of estrogen on the epididymis of prepubertal rhesus monkey.
An estrogen binding protein has been demonstrated in the cytosol of the epididymis and caudal lobe of prostate of immature rhesus monkey. Scatchard plot analyses were linear, suggesting a single class of high affinity binding sites for the synthetic estrogen, R-2858 (Moxestrol) with a Kd of 5.88 X 10(-10) mol/l and a concentration of binding sites of 17.0 +/- 4.4 fmol/mg cytosol protein in the epididymis. In the prostate, the concentration of binding sites was 19.7 +/- 3.6 fmol/mg cytosol protein with a Kd of 5.42 X 10(-10) mol/l. In immature castrated monkeys, administration of estradiol dipropionate caused a significant increase in the weight, total protein and sialic acid content of the epididymis. This biological action of estrogen on the epididymis is presumably mediated via estrogen receptor.
Animals↗
Zinc in the seminal vesicle and cranial and caudal prostate of rhesus monkey (Macaca mulatta): testicular regulation and subcellular distribution.
Zinc concentration and its subcellular distribution in the seminal vesicle and cranial and caudal prostate of adult rhesus monkey was determined by atomic absorption spectroscopy. Its concentration (microgram/mg protein) was maximum in the caudal lobe of prostate (5.7 micrograms) followed by cranial prostate (2.8 micrograms) and seminal vesicle (1.26 microgram). Analysis of subcellular fractions revealed that zinc concentration was highest in the microsomal fraction constituting about 50% of total zinc in the two lobes of prostate (cranial: 5 micrograms; caudal: 17 micrograms/mg protein). A significant reduction occurred in their zinc content following castration. The possible role of zinc in the accessory sex glands is discussed.
Animals↗
Effect of vasectomy on the ultrastructure of epididymal epithelium in rhesus monkey.
Ultrastructural changes in the epididymal epithelium and the fate of accumulating spermatozoa were examined in the vasectomized rhesus monkey (Macaca mulatta). Accumulation of spermatozoa resulted in an increase in the diameter of the tubule and its lumen. Ultrastructure of principal cells revealed that they continue to perform both secretory and absorptive functions after vasectomy. The rough endoplasmic reticulum, Golgi complex, and mitochondria were well developed in the principal cell. Bulging of the apical portion of principal cells and membrane-bound structures in the lumen suggests an increase in apocrine secretion. An increase in the number of vesicles, vacuoles, and multivesicular bodies in the principal cells indicates an increased absorptive activity. Increased absorptive function was also evident in the apical cells. Macrophages with sperm remnants were seen in the lumen, and occasionally in the connective tissue. The principal or only mechanism of sperm disposal after vasectomy appeared to be intraluminal endocytosis by macrophages.
Animals↗
Ultrastructure of the epididymal epithelium of rhesus monkey (Macaca mulatta).
The regional fine structure of the epithelium lining the epididymis of the rhesus monkey has been investigated. Tall, prismatic principal cells constituted the major part of the epithelium. Their basal plasma lemma showed presence of caveolae and the luminal surface was studded with stereocilis. Presence of numerous desmosomes and tight junctions between adjacent principal cells suggested the existence of blood epididymis barrier. Ultrastructural evidence is presented in support of high lysosomal turnover, absorptive and secretory functions in these cells. Apocrine secretion was evident only in the principal cells of initial segment. Of the two types of basal cell, dark and pale, the latter revealed presence of lipofuscin pigment suggesting their scavenger role in the epithelium.
Animals↗
Effect of TSAA-291 on male reproductive tract and fertility of the rat.
Adult male rats were injected with TSAA-291 (16 beta-ethyl-17 beta-hydroxyestr-4-en-3-one), a steroidal antiandrogen, daily for 30 days at two doses, viz., 10.0 and 25.0 mg/kg. The treatment caused a dose-dependent reduction in the weights of testis, epididymis, and other accessory sex glands. In the animals that received the high dose, 50% of the rats showed spermatogenic arrest in about 20% of the seminiferous tubules and Leydig cell morphology was normal. The levels of glycerylphosphorylcholine and sialic acid were significantly reduced in the cauda epididymis of all the treated rats. There was a reduction in the number of motile spermatozoa in the cauda epididymis of rats treated with high dose of the antiandrogen. This was accompanied by a reduced number of females exhibiting spermatozoa in their vaginal smears. A combination treatment of the antiandrogen and androgen appears to be a promising approach for fertility regulation in the male.
Androgen Antagonists↗
Ultrastructural changes in the principal cells of epididymis of adult rhesus monkey (Macaca mulatta) after castration and androgen replacement therapy.
The epididymal epithelium from adult castrated and androgen supplemented, castrated rhesus monkeys was examined with transmission electron microscope. Ninety days after bilateral castration the tubular diameter of all the regions of the epididymis, viz. the initial segment, caput, corpus and cauda portions of epididymis was reduced and was accompanied by a drop in cell size. There was a marked decrease in the number and size of microvilli on the luminal surface. The invaginations of apical membrane into the cell cytoplasm became less prominent. There was a reduction in the amount of smooth and rough endoplasmic reticulum, and Golgi complex. Mitochondria accumulated in the apical cytoplasm. Several vacuoles often associated with lipofuscin pigment granules were common in the cytoplasm. These findings suggest a reduction in both the absorptive and secretory functions of the principal cells. Following androgen replacement therapy for 30 days in monkeys that were castrated 60 days earlier there was a recovery of structural features in the epithelium to near normal state.
Animals↗
Epididymal basal cells in rhesus monkey (Macaca mulatta): ultrastructural changes after castration and androgen replacement therapy.
The ultrastructure of epididymal basal cells in adult castrated and castrate-androgen supplemented rhesus monkey was studied. Due to reduction in the height of the epithelium, three months after castration their number appeared to have increased. In the initial segment dark basal cells occupied more area in the epithelium and their cytoplasm showed the presence of large vacuoles. Cells that resembled dark basal cell were found at all heights of the epithelium. In all the segments, dark basal cells developed pseudopod-like structures. Pale basal cell cytoplasm was filled with lipofuscin pigment granules. Androgen replacement therapy for 30 days prior to autopsy at 90 days did not bring about any significant changes in the ultrastructure of both the types of basal cell. However, the size of the dark basal cell appeared to have decreased. The possible role of basal cells in the disposal of products accumulated in the principal cells under normal and altered endocrine conditions in discussed.
Animals↗
Effect of centchroman on the genital organs of the offspring of rats when administered during lactation or during the neonatal period: a comparative study with ethynylestradiol and diethylstilbestrol.
Lactating rats were treated with centchroman (1.25 mg/kg body wt. p.o.) or ethynylestradiol (100 micrograms/kg p.o.) daily from day 1 of parturition for 21 days and their offspring were autopsied at 60, 90 and 120 days of age. In the offspring nursed by centchroman treated mothers there was no change in ovarian weight. There was a reduction in their uterine weight; while the luminal epithelium presented a stimulated state, the stroma showed immature state in its histological features. The ovarian and uterine weights and their histology in the offspring of ethynylestradiol treated mothers showed no change. The male offspring of both the groups of rats showed an increase in the weight of genital organs and testis histology presented typical adult features. When centchroman was injected s.c. on 5th postnatal day to female rats (500 micrograms/rat), the ovary remained normal but the uterus revealed estrogen deficient state when they were 60 or 90 days old. Neonatal female rats injected with diethylstilbestrol (10 micrograms/rat) on day 5 showed normal ovary while uterus presented features typical of estrogenic stimulation with small areas of luminal epithelium showing squamous metaplastic changes. The results are discussed comparing the centchroman effects with the reported findings in rats treated with steroidal and triphenylethylene estrogenic compounds during lactation or the neonatal period.
Age Factors↗