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Biomedical subjects

B S Uteshev

Publications and source records attributed to B S Uteshev.

At least 19 recordsLinked to original sources
PubMed1992

[Immunomodulating action of heteropolysaccharides isolated from camomile flowers].

Administration of heteropolysaccharides from the camomile flower clusters to rats which failed to perform a physical load (swimming) resulted in stimulation of development of the immune response to SRBCs. However, it did not influence development of the immune response to a bacterial lipopolysaccharide in the rats. A short-term exposure of the swimming animals to high doses of the heteropolysaccharides increased development of the immune response induced by their lipopolysaccharide. A long-term exposure of the swimming rats to low doses of the heteropolysaccharides increased development of the immune response to SRBCs and the lipopolysaccharide. The high doses of the heteropolysaccharides induced excretion of the helper factors by the spleen cells not adhesive to glass while the low doses of the heteropolysaccharides decreased sensitivity of the cells of the immune system to the influence of the suppressing factor excreted by the glass-adherent spleen cells from swimming rats.

Animals↗
PubMed1990

[The phagocytic activity of the macrophages from mouse peritoneal exudate under the action of platinum preparations].

The influence of new antiblastic coordination compounds oxoplatinum and cycloplatam on murine peritoneal macrophage phagocytic activity was investigated in chemiluminescence test in vivo in vitro. The study has shown the stimulation of peritoneal macrophage activity by oxoplatinum and cycloplatam in vitro. In all days, except the first one, oxoplatinum and cycloplatam stimulated macrophage function of intact and especially immune mice.

Animals↗
PubMed1979

[Comparative characteristics of the antitumor and immunodepressive activity of carminomycin on the L-1210 experimental model].

The antitumor activity of carminomycin was estimated by the number of lymphoma colonies formed in the spleen of DBA/2 mice on their inoculation with the bone marrow cells from mice with transplantable leukemia L-1210. The immunodepressive properties of carminomycin were determined by the number of the antibody forming cells in the spleen of CBA and DBA/2 mice with leukemia L-1210 after immunization with sheep red blood cells. It was found that in a single dose of 1.5 mg/kg carminomycin inhibited the lymphoma colonies by 50 per cent. The maximum immunodepressive effect was observed when carminomycin was used in a single dose of 1.5 mg/kg 48 hours after the antigen stimulation. In this case the number of the antibody forming cells in DBA/2 mice with leukemia L-1210 was lower than that in CBA mice without leukemia.

Animals↗
PubMed1978

The kinetics of inhibition with methotrexate and vinblastine of the primary immune response to sheep red blood cells in mice.

Forty-eight or 72 h after immunization with sheep red blood cells, CBA mice were injected intraperitoneally with a single dose of methotrexate or vinblastine, 2.5 mg/kg and 1.5 mg/kg respectively, and then the number of direct plaque-forming cells in their spleen was estimated at 4-h intervals. The PFC growth curve showed an exponential and staircase pattern. After administration of both cytostatics, the PFC numbers in the spleen of mice changed in a wave-like fashion: the increase was accompanied by a decrease. In some parts of the curves obtained the PFC numbers increased or decreased 2 to 7 times within 4 hours. A marked increase in PFC numbers in the controls and in animals treated with both cytostatics occurred at 60, 72--76, 84--88 h of the immune response. It is suggested that methotrexate and vinblastine do not prevent recruitment of precursor cells into PFC and consequently the number of PFC increases. The decrease in PFC numbers under the influence of both cytostatics presumably results from "unbalanced growth".

Animals↗
PubMed1978

[Proliferative kinetics of antibody-forming cells normally and exposed to vinblastine].

The proliferative kinetics of IgM antibody-producing cells (APC) of mice immunized by sheep red blood cells was studied using autoradiography and vinblastine. The incorporation index for APC was determined every 4 hours from 72 to 112 hours of the immune response. The significance of recruitment in APC exponential growth was proven by means of 3 different regimes of 3H-thymidine introduction to immunized mice. The discontinuous character of recruitment and its resistance to vinblastine were shown. It is suggested that vinblastine impared both APC and their precursors.

Animals↗
PubMed1977

[Immunosuppressive properties of vinblastine and colchicine].

The action of vinblastine and colchicine on the populations of antibody-forming cells, their precursors and stem cells using a model of primary immune response and in the transplantation system was studied using techniques by Jerne and Nordin (1963), Kennedy et al. (1965), and Till and McCulloch (1961). It was discovered that vinblastine and colchicine, at single administration 48 hours after immunization, inhibited immune response completely. The administration of colchicine and vinblastine to a donor gave an exponential decrease of in foci-forming cell number in a recipient in the system of syngenic transfer. But at the same time, vinblastine at small doses stimulated population of antibody-forming and stem cells, whereas at large doses it was not effective. Colchicine, in contrast, strongly inhibited both antibody-forming and haemopoietic cells in the recipient.

Animals↗
PubMed1977

[Role of immune RNA in the interaction of T- AND B-lymphocytes].

"Immune" RNA preparations were obtained from the total population and also from the T- and B-lymphocytes of the spleens of the QBA line. Intact bone marrow cells or splenic cells activated with antigen served as target cells for the "immune" RNA. Investigations were carried out in the system of syngenic transfer. To study the target cells in the activated population of the spleen elimination of T-or B-lymphocytes was realized immediately after the incubation of the suspension of the splenic cells with the RNA preparations with the aid of anti-theta-or anti-B-antilymphocytic sera. T-lymphocytes served as the source of the biologically active RNA in the total preparation. B-lymphocytes of the spleen and the bone marrow served as target cells for the RNA of the cells of thymus origin. However, to detect the inducing action of the RNA simultaneous presence in the population of T- and B-lymphocytes is necessary.

Animals↗
PubMed1976

[Kinetics of the immune response following syngenous transfer of bone marrow, thymus and spleen cells from donors under the influence of previously administered antigen].

The influence of the donor priming upon the recipient's immune response under conditons of syngenic transfer of nucleated cells of the bone marrow, thymus, spleen to the lethally irradiated recipients was studied, the recipients being immunized with sheep erythrocytes. A fall of total number of antibody-forming cells was observed in the recipients in priming. The number of hemolytic foci was increased the first day after priming in case of injection of the bone marrow cells from the intact donors and of the thymus cells from the immunized donors. On the third day of the experiment the number of these foci was three time less in comparison with the control. An increase of the hemolytic foci was discovered in the syngenic transfer of splenic cells affected by priming.

Animals↗
PubMed1975

[Study of the immunodepressive effect of passive immunization on a model of adaptive immunity].

The effect of various doses of specific serum administered in passive immunization on the formation of primary and secondary adaptive immunity in mice of the CBA strain was studied. Primary and secondary response was more sensitive to passive immunization on a model of adaptive immunity than in vivo. Most sensitive to the immunodepressive action of specific antibodies IgM and IgG were plaque-forming cells, and most resistant -- rosette-forming cells and cells. This led to a supposition that antibodies administered in passive immunization depressed the development of the immunological response acting at the level of T- and B cells.

Animals↗
PubMed1975

Analysis of the Kinetics of the immune response by means of ionizing radiation.

The inhibitory kinetics of the primary immune response was studied by means of lethal irradiation. CBA mice were immunized with sheep red blood cells and 48, 72 and 96 hours later, they were irradiated with 750R. Afterwards, the number of direct plaque-forming cells was determined in their spleens during 58 to 66 hours at 4-hour intervals. After irradiation at 48 and 72 hours of the immune response, a plateau of different duration was obtained: 48 hours in the former and 24 hours in the latter case. In both cases, reduction in the PFC number began at about 96 hours of the resonse. The PFC reduction curves in these two series of experiments and those obtained 96 hours after irradiation and in control, non-irradiated mice were parallel. The mean half-time of the PFC number reduction as determined by these curves was 6 hours. The time of functioning of PFC in the spleen is discussed with respect to recruitment of proliferating precursors.

Animals↗
PubMed1975

[RNA biosynthesis in the microsomes of splenic cells during the inductive phase of the immunological response].

RNA labeled with 3H-uridine was extracted from the microsomes of the spleen of the intact and antigen-stimulated mice. A study was made of the composition of this RNA by electrophoresis in polyacrylamide gel. Apart from rRNA, there were revealed in the microsome composition up to 10 RNA components in the mol wt range of from 0.4-10(5) to 7-10(5) dalton and 2 componnents - between 7-10(5) and 1.7-10(6) dalton. Incorporation of 3H-uridine into the rRNA was the maximal 24 hours after the administration of the antigen, whereas the RNA with the mol wt between 0.4-10(5) dalton remaining practically unchanged for a period of three days after the immunization. 3H-uridine incorporation into these RNA was resistant to the action of low antibiotic (actinomycin D) doses. Immunization was not accompanied by the appearance of new, by molecular weight, RNA components.

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