Biomedical subjects

B Sanger

Publications and source records attributed to B Sanger.

3 recordsLinked to original sources

PubMed2001

Congenitally anomalous tented eyebrows.

PURPOSE: An uncommon congenital anomaly of the eyebrows is described. METHODS: Case report and review of the literature. RESULTS: Two articles in the English literature were found describing patients similar to ours. CONCLUSIONS: Congenital upward tenting of the midportion of the eyebrows, resembling the eyebrow line of an owl, is uncommon. This anomaly is thought to be transmitted in an autosomal dominant fashion and may be accompanied by myopia and strabismus, but has not been associated with systemic disease.

Child↗

PubMed1983

Sample collection and processing for the assay of plasma renin activity. A comparison of anticoagulants and inhibitors.

Plasma renin activity was measured in samples collected in lithium heparin, sodium heparin, and ethylenediaminetetraacetate. The samples were incubated under two widely used sets of conditions to generate angiotensin I, and the results were compared. Under certain incubation conditions, the absence of ethylenediaminetetraacetate from the sample tube produced low plasma renin activity values. This was obviated by using an alternative incubation medium and was not, therefore, due to direct inhibition of renin by heparin. These lower values were not seen in samples from patients receiving captopril. This suggests that the discrepant values were due to some angiotensin I conversion to angiotensin II and lower homologues in the incubates. Sodium heparin samples compared closely with lithium heparin samples, indicating that the presence of lithium is not significant. These results show that, provided the incubation conditions are suitably modified, samples collected into lithium or sodium heparin can be used for measurement of plasma renin activity.

Captopril↗

PubMed1983

The stability of angiotensin I formed at room temperature in the presence of ethylenediaminetetraacetate to subsequent incubation at 37 degrees C.

It has been reported that plasma renin activity values obtained from samples containing ethylenediaminetetraacetate (EDTA) and which have been allowed to remain at room temperature for 24-48 hours prior to incubation and assay are spuriously low. Since plasma renin activity is often measured in specialised centres, considerable time delays in sample transit may be difficult to avoid. We, therefore, investigated these findings using plasma samples kept at room temperature for varying times and subsequently incubated at 37 degrees C. From these studies we conclude that plasma renin activity is not significantly affected by pre-incubation at room temperature for periods up to 48 hours. However, the precision of the assay is likely to be poorer in specimens pre-incubated at room temperature. These results are supported by the finding that there is no loss of angiotensin I upon incubation and assay of samples containing pepstatin A.

Angiotensin I↗