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B Sh Gil'burd

Publications and source records attributed to B Sh Gil'burd.

8 recordsLinked to original sources
PubMed1991

[The characterization of the spectrum of the antibody response to Mycobacterium tuberculosis antigenic determinants by immunoblotting].

The spectrum of antibody response to M. tuberculosis antigenic determinants H37Rv and M. bovis antigenic determinants BCG was studied in serum samples from 33 healthy donors and 31 patients with infiltrative pulmonary tuberculosis by the method of immunoblotting. The study revealed that most frequently tuberculosis patients showed response to Ag-H37Rv with molecular weights of 52, 39, 35, 21, 31, 68 kD (44.4-22.2%) and Ag-BCG with molecular weights 60, 58, 50, 25, 54, 70 kD. (33.3-22.2%). By month 9 of effective chemotherapy binding predominantly with Ag-H37Rv determinants of 31, 62, 35, 75, 56, 28, 19, 5, 13 kD (75-37.5%) and Ag-BCG determinants of 13, 34, 38, 44, 19, 36, 45, 52, 58, 60 70 kD (37.5-25%) were registered. Some differences in the spectra of antibody response to Ag-H37Rv and Ag-BCG determinants were noted.

Antibodies, Bacterial↗
PubMed1991

[Specific antibodies to different mycobacterial antigens in patients with pulmonary tuberculosis].

Antibodies to three mycobacterial antigens, Soviet tuberculin and ultrasound-treated Mycobacterium tuberculosis (H37Rv) and M. bovis (BCG) were detected by enzyme immunoassay in 90 patients with pulmonary tuberculosis and 75 normal subjects. Antibody detection rates and levels were found related to the form of tuberculous process. The detection rate was rather low in focal tuberculosis and virtually the same for all the antigens (15.3 percent). In disseminated processes (infiltrative and particularly disseminated and fibrous-cavernous) antibodies are detected more often in higher titers than in local processes, and their levels are different with different antigens. The method was more sensitive with ultrasound-treated antigens of M. tuberculosis (H37RV) and M. bovis (BCG) than with PPD. Simultaneous enzyme immunoassay with three antigens detected up to 75 percent of antituberculous antibodies in infiltrative, up to 92 percent of antibodies in disseminated, and up to 100 percent of antibodies in fibrous-cavernous tuberculosis.

Antibodies, Bacterial↗
PubMed1989

[Antitubercular antibodies to antigens of various molecular weights in patients with active pulmonary tuberculosis].

Antibodies were determined in 72 tuberculous patients and 27 healthy donors using enzyme immunoassay (EIA) and immunoblotting (IB). It was shown that the levels of the antibodies to BCG and H37Rv sonicates were lower in the patients with disseminated tuberculosis than in the patients with fibrocavernous or infiltrative tuberculosis. Significant differences in the antibody spectra in the patients with different forms of tuberculosis were also shown. Thus, in the patients with disseminated tuberculosis IB with BCG and H37Rv sonicates revealed only single bands in the region of the reaction with the antigens of low molecular weights. In the patients with infiltrative tuberculosis the reaction with H37Rv or BCG sonicates most frequently revealed the antibodies to the antigen determinants with molecular weights of 50, 30, 41, 54, 52 and 60.43 or 45.5, 40.5, 57 and 52 kD, respectively. In the patients with fibrocavernous tuberculosis the reaction with H37Rv or BCG sonicates most frequently revealed the antibodies to the antigen determinants with molecular weights of 54, 42, 48, 30 and 13.5 or 24, 57, 37 and 14.5 kD respectively.

Adult↗
PubMed1989

[Determination of antibodies in blood serum of patients with pulmonary tuberculosis using mycobacterial antigen affinity isolation].

Possible use of radio- and enzyme immunoassays (RIA and EIA, respectively) in serodiagnosis of tuberculosis with antigen BCG isolated by affinity was studied in comparison to the use of the routine antigen PPD. The specific IgG antibodies were determined in serum specimens of 150 patients with various forms of pulmonary tuberculosis and 50 healthy donors. A statistically reliable difference in the efficiency of tuberculosis serodiagnosis (high levels of specificity and sensitivity) by the RIA and EIA with using antigen BCG isolated by affinity was observed.

Antibodies, Bacterial↗