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Biomedical subjects

B Stanković

Publications and source records attributed to B Stanković.

At least 19 recordsLinked to original sources
PubMed2000

Molecular cloning and characterization of a tomato cDNA encoding a systemically wound-inducible bZIP DNA-binding protein.

Localized wounding of one leaf in intact tomato (Lycopersicon esculentum Mill.) plants triggers rapid systemic transcriptional responses that might be involved in defense. To better understand the mechanism(s) of intercellular signal transmission in wounded tomatoes, and to identify the array of genes systemically up-regulated by wounding, a subtractive cDNA library for wounded tomato leaves was constructed. A novel cDNA clone (designated LebZIP1) encoding a DNA-binding protein was isolated and identified. This clone appears to be encoded by a single gene, and belongs to the family of basic leucine zipper domain (bZIP) transcription factors shown to be up-regulated by cold and dark treatments. Analysis of the mRNA levels suggests that the transcript for LebZIP1 is both organ-specific and up-regulated by wounding. In wounded wild-type tomatoes, the LebZIP1 mRNA levels in distant tissue were maximally up-regulated within only 5 min following localized wounding. Exogenous abscisic acid (ABA) prevented the rapid wound-induced increase in LebZIP1 mRNA levels, while the basal levels of LebZIP1 transcripts were higher in the ABA mutants notabilis (not), sitiens (sit), and flacca (flc), and wound-induced increases were greater in the ABA-deficient mutants. Together, these results suggest that ABA acts to curtail the wound-induced synthesis of LebZIP1 mRNA.

Abscisic Acid↗
PubMed2000

Low serum alpha-1-antitrypsin specific activity in monoclonal gammopathies.

BACKGROUND: Serum alpha 1-antitrypsin (alpha 1AT) antigen concentration is elevated in malignancies as the result of acute phase reaction. In the present study, we examined whether the alpha 1AT elevation in monoclonal gammopathies was accompanied by an adequate increase of its functional activity. METHODS: In this case-control study, serum alpha 1AT concentration was measured in 187 ambulatory patients with monoclonal gammopathies and 320 healthy blood donors matched according to sex and age. The alpha 1AT antigen concentration was assayed by immunonephelometry, whereas its functional activity was measured as trypsin inhibitory capacity (TIC). The specific alpha 1AT inhibitory activity (SIA) was calculated, defined as the TIC/antigen concentration ratio. RESULTS: The alpha 1AT antigen concentrations obtained in the patients' samples were very significantly higher as compared with the corresponding values in the control group (mean +/- SD = 134 +/- 41.9% of normal, p < 0.001). However, the TIC values were higher in the patients than in the healthy controls only by 4% (104 +/- 23.8%, p < 0.05). The specific alpha 1AT activity was very significantly lower in the patients, as compared with the controls (p < 0.001), indicating that serum alpha 1AT in monoclonal gammopathies was partially inactive. CONCLUSIONS: As poor correlation between the TIC values and the antigen concentrations was obtained in the patient group as well as the decreased specific alpha 1AT activities, the TIC values in patients with monoclonal gammopathies should be interpreted with caution.

Adult↗
PubMed1996

Both action potentials and variation potentials induce proteinase inhibitor gene expression in tomato.

Tomato plants (Lycopersicon esculentum) accumulate proteinase inhibitor 2 (pin2) mRNA in response to insect attack, crushing and flaming in leaves distant from those treated. Most earlier work suggests that the systemic wound signals are chemical; here we try to determine whether electrical or physical (hydraulic) signals can also evoke pin expression. We used a mild flame to evoke a systemic hydraulic signal and its local electrical aftermath, the variation potential (VP), and we used an electric stimulus to evoke a systemic electrical signal, the action potential (AP). We determined the kinetic parameters of both the VP and AP. Flame-wounded plants essentially always exhibited major electrical responses throughout the plant and a several-fold increase in pin2 mRNA within 1 h. Electrically stimulated plants that generated and transmitted a signal (AP) into the analyzed leaf exhibited similarly large, rapid increases in pin2 mRNA levels. Plants which generated no signal, or signals of just a few microvolts, had unchanged levels of pin2 mRNA. Since the AP and VP both arrived in the receiving leaf before accumulation of pin2 mRNA began, we conclude that, in addition to the previously shown chemical signals, both hydraulically induced VPs and electrically induced APs are capable of evoking pin2 gene expression.

Action Potentials↗
PubMed1994

Jackson-Weiss syndrome registered in four successive generations. The facies of Crouzon's syndrome with foot abnormalities.

A family with 15 individuals in four successive generations affected by Jackson-Weiss syndrome, craniosynostosis with Crouzon-variant-like phenotype and feet's abnormalities, is presented. An autosomal dominant inheritance pattern with complete penetrance, variable expressivity, and wide intrafamilial variation, more among, less within the same generation, was observed. Concerning the frequency and severity of complications, the evolution of craniofacial deformities seems to parallel those described with Crouzon syndrome, suggesting the similar evaluation and management.

Adult↗
PubMed1994

[Acute normovolemic hemodilution].

Performing acute normovolemic hemodilution reduces needs for transfusion of stored homologous blood, exposure of patients to homologous blood and incidence of diseases transmitted by transfusion. Apart from that it provides significant quantities of immunologically best, completely fresh blood and/or some blood components necessary for transfusion. Apart from the latest literature data, in this paper represent the first experiences considering acute normovolemic hemodilution performed in 20 patients operated for having low localized colon's neoplasm in whom amputation of rectum was performed. 780 ml of blood was exfunded from each patient with simultaneous infusion of three times larger volume of lactated Ringer's solution (In 10 patients) one volume of Haemacell (in 5 patients) and one volume of Soludex 70 (in 5 patients). Considering these patients 15600 ml autologous and 2500 ml of homologous blood was used up for transfusion.

Adult↗
PubMed1990

Potentiometric investigation of the stability of palladium(II) complex of pralidoxime chloride in aqueous solution.

The formation of a complex between palladium(II) chloride and pralidoxime chloride (PAM-2Cl) has been studied by means of potentiometric pH measurements. The real stability constant of the complex in aqeous medium of ionic strength 0.3 M (KCl) at 25.0 degrees C was log Ks = 7.29. This value was close to that (log Ks = 7.02) obtained previously by spectrophotometric methods after appropriate correction with respect to the corresponding value of the acidic constant of PAM-2Cl (pKca = 8.05), which was also determined under the same experimental conditions.

Drug Stability↗
PubMed1990

Spectrophotometric investigation of complex formation of an oxime PAM-4Cl with palladium (II) and its analytical application.

The colour reaction of 4-hydroxyiminomethyl-1-methylpyridinium chloride (PAM-4Cl) and palladium(II) chloride has been investigated. The optimum reaction conditions, spectral characteristics, conditional stability constant and composition of the yellow water-soluble complex have been established. A new spectrophotometric method is proposed for the microdetermination of PAM-4Cl.

Chemical Phenomena↗
PubMed1990

[Fluoridation of drinking water in Pancevo].

In Pancevo on May 12, 1988 the continual fluoridation of water was started. Fluoridation is conducted with 2% silicofluoride acid. Up to now, controls on fluoride content in drinking water, which are conducted on eight places in the city, show that the concentration of fluoride is about 1 mg of fluoride per liter of drinking water. There are very little deviations during measurement, so the system can be considered as safe. The analysis of costs of water fluoridation in October 1988 was 250 dinars per inhabitant. In this way we achieved the most efficient and at the same time cheap method of dental health protection as well as the protection of the whole human organism, in accordance with the opinions of the World Health Organization concerning that subject.

Fluoridation↗
PubMed1988

Use of palladium(II) chloride as colour-forming reagent in determination of pralidoxime chloride in water and tablets.

Pralidoxime chloride (PAM-2Cl) has been determined spectrophotometrically in Britton-Robinson buffer solution at pH = 6.45; the method is based on measurement of the absorbance of the Pd(II)-pralidoxime complex at 327 nm. Studies of the composition of the complex by Job's continuous variation method, the molar ratio method and Bent-French's method yielded a Pd(II):pralidoxime ratio of 1:1. The conditional stability constant (K') of the complex at the optimum pH of 6.45 and an ionic strength (mu) of 0.3 M was found to be 10(5.2). The molar absorptivity was 1.05 x 10(4) 1 mol(-1) cm(-1). Beer's law was obeyed at concentrations up to 60 microM. The detection limit was 0.55 microg ml(-1). The relative standard deviation (N = 10) was 0.28-1.03%. The method was accurate and sensitive for the analysis of PAM-2Cl in water and tablets.

Journal Article↗
PubMed1987

Spectrophotometric determination of obidoxime chloride as its Pd(II) complex in water and injections.

It has been established that obidoxime chloride reacts with palladium(II) chloride in the pH range 3.9-8.0 and forms a yellow water-soluble (1:1) complex with maximum absorbance at 355 nm. By applying the methods of Sommer, Nash and Job involving non-equimolar solutions the conditional stability constant of the complex, at the optimum pH of 6.3 and an ionic strength mu = 0.55 M, is found to be 10(5.3). The molar absorptivity at 355 nm is 3.16 x 10(4) l mol(-1) cm(-1) at pH 6.3. Beer's law is obeyed up to 20 mumol l(-1) obidoxime chloride concentration. The detection limit of the method is 0.36 mug ml(-1). The relative standard deviation (n = 10) varies over the range 1.10-2.66%. The proposed method was found to be suitable for the accurate and sensitive analysis of obidoxime chloride in water and injection solutions.

Journal Article↗