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Biomedical subjects

B Sun

Publications and source records attributed to B Sun.

At least 235 records · Page 13Linked to original sources
PubMed1996

[Comparison of various types of imidazole derivatives for treatment of filamentous fungal keratitis].

OBJECTIVE: 73 eyes of 73 patients with filamentous fungal keratitis were treated by various types of imidazole derivatives. METHODS: The mean patient age was 36 (range, 15-62) years. The areas of corneal ulcer were 2-4 mm x 2-5 mm. The hypopyon was 1-3 mm in height. The patients were randomly divided into 4 groups: I 23 eyes were treated by 1% miconazole suspension. II 15 eyes by imidazole mixed oil (i.e. econazole and miconazole in castor oil). III 19 eyes by dusting mixed imidazole powder onto focal corneal lesions. IV 16 eyes by focal corneal lesion resection combined with dusting mixed imidazole powder. RESULTS: The cure rates were 21.74%, 66.67%, 73.68% and 93.75%, and the average days of treatment were 65, 68, 35 and 19, respectively, in the 4 groups. The differences in the cure rates and in the means of treatment durations between each of the former three groups and the 4th group are remarkable (P <0.01). CONCLUSION: Focal corneal lesion resection combined with dusting mixed imidazole powder is relatively effective in treatment of filamentous fungal keratitis.

Adolescent↗
PubMed1996

[Extended vertical partial laryngectomy for treatment of the glottic cancer with T3 category].

From June 1981 to December 1992, 32 patients of glottic cancer with T3 category were treated by extended vertical partial laryngectomy, i.e. vertical laryngectomy plus the resection of the arytenoid and part of the cricoid plate. The laryngeal defect was reconstructed by an osteomuscular flap. The 3- and 5-year survival rates were 78.1% and 73.9% respectively. The decannulation rate was 81.3%. The voice was satisfactory in 84.3%. All patients resumed mouth-food-taking. The authors conclude that selective glottic cancer of T3 category treated with extended vertical partial laryngectomy, which preserves the functions of the larynx and improves the quality of the life, is feasible and acceptable.

Adult↗
PubMed1996

[Speech restoration via a tracheo-esophageal tegmental valve fistula following total laryngectomy].

In order to restore the patients' speech function after total laryngectomy, the speech restoration operation has been done successfully in 12 patients with laryngocarcinomas by making tegmental valve fistula from esophageal flap and tracheal tissue after total laryngectomy. First, the trachea and the esophagus were separated, and a tissue flap 20mm in length from posttracheal wall was made. Its upper end was 10mm in width, lower end 8 mm, laterally 5 mm x 6 mm, the tracheal mucosa and cartilage were sewn up to from a tegmental valve, then, a tissue flap 20 mm in length from the preesophageal wall was done with its upper end 15 mm in width, lower end 10 mm. Then a tegmental valve fistula (speech-tube) was made by sewing up two flaps crisscross and a 12F catheter was inserted into the tegmental valve fistula as a supporter for 10 days. Finally, a tracheostomy was performed by sewing up the trachea to the neck skin. All patients could speak fluently and clearly after surgery and kept their sex and dialect characteristics whereas without misswallowing. The operation is a perfect way for total laryngectomy with speech restoration in patients with upper glottal or glottal laryngocarcinomas.

Adult↗
PubMed1995

Studies on the catalytic mechanism of five DNA glycosylases. Probing for enzyme-DNA imino intermediates.

DNA glycosylases catalyze scission of the N-glycosylic bond linking a damaged base to the DNA sugar phosphate backbone. Some of these enzymes carry out a concomitant abasic (apyrimidinic/apurinic(AP)) lyase reaction at a rate approximately equal to that of the glycosylase step. As a generalization of the mechanism described for T4 endonuclease V, a repair glycosylase/AP lyase that is specific for ultraviolet light-induced cis-syn pyrimidine dimers, a hypothesis concerning the mechanism of these repair glycosylases has been proposed. This hypothesis describes the initial action of all DNA glycosylases as a nucleophilic attack at the sugar C-1' of the damaged base nucleoside, resulting in scission of the N-glycosylic bond. It is proposed that the enzymes that are only glycosylases differ in the chemical nature of the attacking nucleophile from the glycosylase/AP lyases. Those DNA glycosylases, which carry out the AP lyase reaction at a rate approximately equal to the glycosylase step, are proposed to use an amino group as the nucleophile, resulting in an imino enzyme-DNA intermediate. The simple glycosylases, lacking the concomitant AP lyase activity, are propose to use some nucleophile from the medium, e.g. an activated water molecule. This paper reports experimental tests of this hypothesis using five representative enzymes, and these data are consistent with this hypothesis.

Animals↗
PubMed1995

Two Drosophila nervous system antigens, Nervana 1 and 2, are homologous to the beta subunit of Na+,K(+)-ATPase.

A nervous system-specific glycoprotein antigen from adult Drosophila heads, designated Nervana (Nrv), has been purified on the basis of reactivity of its carbohydrate epitope(s) with anti-horseradish peroxidase (HRP) antibodies that are specific markers for Drosophila neurons. Anti-Nrv monoclonal antibodies (mAbs), specific for the protein moiety of Nrv, were used to screen a Drosophila embryo cDNA expression library. Three cDNA clones (designated Nrv1, Nrv2.1, and Nrv2.2) were isolated that code for proteins recognized by anti-Nrv mAbs on Western blots. DNA sequencing and Southern blot analyses established that the cDNA clones are derived from two different genes. In situ hybridization to Drosophila polytene chromosomes showed that the cDNA clones map to the third chromosome near 92C-D. Nrv1 and Nrv2.1/2.2 have open reading frames of 309 and 322/323 amino acids, respectively, and they are 43.4% identical at the amino acid level. The proteins deduced from these clones exhibit significant homology in both primary sequence and predicted topology to the beta subunit of Na+,K(+)-ATPase. Immunoaffinity-purified Nrv is associated with a protein (M(r) 100,000) recognized on Western blots by anti-ATPase alpha-subunit mAb. Our results suggest that the Drosophila nervous system-specific antigens Nrv1 and -2 are neuronal forms of the beta subunit of Na+,K(+)-ATPase.

Amino Acid Sequence↗
PubMed1995

Ultrabithorax protein is necessary but not sufficient for full activation of decapentaplegic expression in the visceral mesoderm.

To elucidate the mechanisms by which homeotic selector (HOM) genes specify the unique features of Drosophila segments, we have analyzed the regulation of decapentaplegic (dpp), a transforming growth factor (TGF)-beta superfamily member, and have found that the Ultrabithorax (Ubx) HOM protein directly activates dpp expression in parasegment 7 (PS7) of the embryonic visceral mesoderm. Other factors are also required, including one that appears to act through homeodomain protein binding sites and may be encoded by extradenticle (exd). The exd protein binds in a highly co-operative manner to regulatory sequences mediating PS7-specific dpp expression, consistent with a genetic requirement for exd function in normal visceral mesoderm expression of dpp. A second mechanism contributing to PS7 expression of dpp appears not to require Ubx protein directly, and involves a general visceral mesoderm enhancer coupled to a spatially specific repression element. Thus, even in an apparently simple case where visceral mesoderm expression of the dpp target gene mirrors that of the Ubx HOM protein, full activation by Ubx protein requires at least one additional factor. In addition, a distinct regulatory mode not directly involving Ubx protein also appears to contribute to PS7-specific expression.

Animals↗
PubMed1995

Characterization of nervana, a Drosophila melanogaster neuron-specific glycoprotein antigen recognized by anti-horseradish peroxidase antibodies.

Antibodies to the plant glycoprotein horseradish peroxidase (HRP) are used extensively to identify neurons in Drosophila and other insects. We are interested in characterizing the gene product(s) recognized by anti-HRP antibodies because it may be important for nervous system function and/or development. Here we identify and purify from adult Drosophila heads an anti-HRP-reactive Mr 42K glycoprotein that is likely to be the major contributor to neuronal specific anti-HRP staining. Several different monoclonal antibodies to the purified 42K glycoprotein recognize up to three proteins with distinct mobilities between Mr 38K and 42K that vary as a function of developmental age. We have collectively named these components Nervana (nerve antigen), because the monoclonal antibodies also specifically stain cultured neurons and embryonic nervous system with a pattern indistinguishable from anti-HRP staining. Western blots indicate the presence of immunologically similar proteins in a wide variety of insect species and in nac (neurally altered carbohydrate) mutant Drosophila flies that lack anti-HRP staining in adult nervous system. It should now be possible to undertake a full biochemical and functional characterization of Nervana in Drosophila.

Aging↗
PubMed1995

Characterization of benzo[a]pyrene metabolism and related cytochrome P-450 isozymes in Syrian hamster livers.

Cytochrome P-450 monooxygenases of golden Syrian hamsters were characterized with respect to benzo[a]pyrene metabolism. Male hamsters were treated with phenobarbital, 3-methylcholanthrene, dexamethasone, benzoflavone, or ethanol, and the activity of aryl hydrocarbon hydroxylase and benzo[a]pyrene activation was determined by mutagenicity testing in hepatic microsomes. Aryl hydrocarbon hydroxylase activity was induced markedly by treatment with phenobarbital but not with 3-methylcholanthrene, nor with other chemicals. The degree of benzo[a]pyrene activation on mutagenicity testing was significantly elevated by treatment with 3-methylcholanthrene and phenobarbital but was reduced with dexamethasone. Immunoinhibition of these activities and Western blotting of hepatic microsomes using antibodies against cytochrome P-450 isozymes suggested that the isozymes responsible for benzo[a]pyrene metabolism in Syrian hamsters belong to the CYP1A, CYP2A, and CYP3A families, a result that differs from observations in rats.

Animals↗
PubMed1995

X region deletion variants of hepatitis B virus in surface antigen-negative infections and non-A, non-B hepatitis.

The etiology of non-A, non-B hepatitis (NANBH) in renal dialysis patients was determined. Hepatitis C virus was present in many, but its appearance did not correlate with elevated alanine aminotransaminase. When sera from these patients were tested for antibodies against hepatitis B virus (HBV) X antigen and polymerase, 70% were positive. HBV infection was confirmed by polymerase chain reaction using several HBV-specific primer pairs. However, amplification with X region primers failed to yield products in many patients. Cloning and sequencing of these products demonstrated deletions within the X region. Hence, X-deletion variants of HBV are strongly associated with NANBH in renal dialysis patients.

Alanine Transaminase↗
PubMed1995

Alveolar-to-vascular leakage of surfactant protein A in ventilated immature newborn rabbits.

We measured alveolar-to-vascular leakage of surfactant protein A (SP-A) in immature newborn rabbits delivered at a gestational age of 27 days. Experimental animals received, via a tracheal cannula, 2 ml/kg of a mixture of modified porcine surfactant (Curosurf, 80 mg/ml) and human recombinant SP-A (4 mg/ml). Littermate controls received the same volume of human SP-A in saline (4 mg/ml). After 30 min of artificial ventilation with a frequency of 40/min and an inspiration time of either 0.75 or 0.45 s, blood was sampled from the right ventricle and the lungs were lavaged. The content of human SP-A in serum and lung lavage fluid was determined with ELISA kits, and the alveolar-to-vascular leak expressed as the quotient of total SP-A in serum and lavage fluid. The leak in control animals amounted to about 2% of SP-A in lung wash and was several times higher in these animals than in those receiving surfactant. The leak was of the same order irrespective of whether the animals were ventilated with long or short inspiration time. We speculate that serum levels of SP-A may reflect the degree of lung injury in various forms of respiratory failure.

Animals↗
PubMed1995

[Influence of PGE2 on the oxidative modification of LDL and the scavenger receptor activities of macrophages].

The influence of oxidized LDL (OX-LDL) on scavenger receptor of macrophages and the protective effects of PGE2 and selenium was studied by means of BA-ELISA technique, immunohistochemical methods and oil red O histochemistry. The results indicated that the scavenger receptor activities of macrophages in two treated groups were much lower than that of OX-LDL group. Oil red O staining showed that the macrophages in OX-LDL group were full of fatty drops and became foam cells while little lipid could be found in the treated groups. Immunohistochemical study revealed that the entry of OX-LDL into the macrophages was by way of scavenger receptors. The results confirmed that both PGE2 and selenium had marked antioxidizing effects and that PGE2 was slightly more effective than selenium.

Animals↗
PubMed1995

[Full thickness lamellar keratoplasty with viscodelamination of cornea for treatment of bullous keratopathy].

Intrastromal injection of viscoelastic (2% methylcellulose or healon) will facilitate the separation of deep stroma during dissection of recipient stroma. Full thickness lamellar keratoplasty was performed on a smooth graft bed which approached Descemet's membrane. 21 cases with bullous keratopathy were treated and followed up for six months, post-operatively. The symptoms were controlled in all cases and 13 cases were relieved from blindness with corrected visual acuities over 0.05. The safety, efficacy and reoperability of full thickness lamellar keratoplasty with viscodelamination of cornea for treatment of bullous keratopathy make it a promising technique.

Adolescent↗
PubMed1995

[Dynamics of IL-2 and IFN-gamma levels induced by sea or Con A in spleen cells of Schistosoma japonicum-infected mice].

The capacity of mitogen Con A and SEA-stimulated spleen cells to produce cytokines IFN-gamma and IL-2 was studied in S. japonicum-infected mice every two weeks from 0 to 14 wk after infection. The results showed that the levels of these two cytokines began to rise at the 4th wk after infection and reached a peak level at 6-8 wk, then declined to the levels similar to those pre-infection at 12-14 wk after infection. The IFN-gamma level reached the peak earlier than the IL-2 level. The dynamics of cytokine level in both mitogen and antigen-stimulated group was similar. The results suggest that IL-2 and IFN-gamma might be the essential cytokines involved in egg granuloma formation in schistosomiasis japonica.

Animals↗
PubMed1995

[Peak expiratory flow rate (PEF) and serum eosinophil cationic protein (ECP) changes in nocturnal asthmatics].

We have investigated the serum ECP and peak expiratory flow rate in 20 patients with nocturnal asthma. Changes of PEF were measured in every 2 hours around the whole day, and the blood samples were obtained at 4:00 and 16:00 to measure the serum ECP level and the peripheral Eo numbers. In addition, 10 asthmatics as well as normal subjects received methacholine challenge at 4:00 and 16:00. It was found that the PEF reached the lowest point at 4:00 and obviously less than that at 16:00 (187.50 +/- 120.31 L/min vs 313.00 +/- 108.14 L/min, P < 0.05), that the airway reactivity at 4:00 was significantly higher than at 16:00 (P < 0.05) and the difference of MCH-PC20 between the two time points was 0.34 +/- 0.31 mg/ml, that the serum ECP level at 4:00 was obviously higher than that at 16:00 (11.14 +/- 7.40 micrograms/ml vs 5.49 +/- 4.12 micrograms/ml, P < 0.05). The change rate of PEF markedly related to the change of serum ECP between the two time points (r = 0.61, P < 0.05). The findings suggested that the activation of Eo and its release of ECP might be effect of the circadian-rhythmic change of pulmonary functions in nocturnal asthma.

Adult↗
PubMed1995

Effects of hypoxic endothelial cell conditioned medium on proliferation and collagen synthesis of smooth muscle cells and inhibitory effects of radix salviae miltiorrhizae.

The effects of hypoxic endothelial cell conditioned medium (HECCM) on proliferation and collagen synthesis of cultured porcine pulmonary arterial smooth muscle cells (PASMCs) were studied by 3H-thymidine (3H-TdR) and 3H-proline incorporations, image analysis for determination of DNA content and colorimetric assay using MTT, and the inhibitory effects of radix salviae miltiorrhizae (RSM) on them were also investigated. The results showed that HECCM could induce enhancement of the enzymatic activity of mitochondria, increase of the nucleic DNA content and increases of the 3H-TdR and 3H-proline incorporation in PASMCs. The 3H-proline incorporation in PASMCs cultured in HECCM was 1.83 times as much as that cultured in normoxic endothelial cell conditioned medium (NECCM). Compared with the control, Chinese herb medicine RSM could inhibit the proliferation of PASMCs cultured in HECCM and decrease the 3H-proline incorporation in PASMCs cultured in both HECCM and NECCM (P < 0.001). However, RSM had no effects on the nucleic DNA content and 3H-TdR incorporation into DNA of PASMCs cultured in NECCM. It suggests that hypoxia may stimulate the endothelia to synthesize and secrete some cytokines which can stimulate the proliferation and the synthesis of collagen of PASMCs and RSM can inhibit this process.

Animals↗