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Biomedical subjects

B T Kelly

Publications and source records attributed to B T Kelly.

11 recordsLinked to original sources
PubMed2005

In vivo healing after capsular plication in an ovine shoulder model.

Traditionally, arthroscopic management of shoulder instability has been reserved for patients with isolated Bankart lesions without any capsular laxity or injury. To date, there are no animal studies evaluating the healing potential of capsular plication and/or capsulo-labral repair. The purpose of this in vivo animal study was to determine if the histological capsular healing of an open capsular plication simulating an arthroscopic plication is equivalent to the more traditional open capsular shift involving cutting and advancing the capsule. Twenty-six skeletally mature sheep were randomized to either an open capsular plication simulating arthroscopic plication (n = 13), or an open traditional capsular shift (n = 13). A sham operation (n = 4) was also performed involving exposure to visualize the capsule. Normal non-operated control shoulders were also analyzed. A pathologist blinded to the treatment evaluated both hematoxylin and eosin (H&E) sections and polarized light microscopy. Qualitative scoring evaluated fibrosis, mucinous degeneration, fat necrosis, granuloma formation, vascularity, inflammatory infiltrate and hemosiderin (0 to 3 points). Both the capsular plication and open shift groups demonstrated healing by fibrosis at the site of surgical manipulation. There were no statistical differences in the capsular healing responses between the two groups with regard to fibrosis, granuloma formation and vascularity. The open shift group demonstrated significantly more mucinous degeneration (p = 0.038). Fat necrosis was present in 4/13 specimens in the open shift group and none in the capsular plication specimens. Both groups demonstrated disorganized collagen formation under polarized light microscopy. There were no differences between non-operated control specimens and sham surgery specimens. Our findings support the hypothesis that histologic capsular healing is equivalent between the plication group and the open shift group. In addition, the open shift group demonstrated significantly more changes indicative of tissue injury. This basic science model confirms capsular healing after simulated arthroscopic plication, providing support for arthroscopic capsular plication in practice.

Animals↗
PubMed2003

Using faecal DNA sampling and GIS to monitor hybridization between red wolves (Canis rufus) and coyotes (Canis latrans).

The US Fish and Wildlife Service's (USFWS) Red Wolf Recovery Program recognizes hybridization with coyotes as the primary threat to red wolf recovery. Efforts to curb or stop hybridization are hampered in two ways. First, hybrid individuals are difficult to identify based solely on morphology. Second, managers need to effectively search 6000 km(2) for the presence of coyotes and hybrids. We develop a noninvasive method to screen large geographical areas for coyotes and hybrids with maternal coyote ancestry by combining mitochondrial DNA sequence analysis of faeces (scat) and geographic information system (GIS) technology. This method was implemented on the Alligator River National Wildlife Refuge (1000 km(2)) in northeastern North Carolina. A total of 956 scats were collected in the spring of 2000 and 2001 and global positioning system (GPS) coordinates were recorded. Seventy-five percent of the scats were assigned to species and five coyote/hybrid scats were detected. Placement of scat location coordinates on a map of the experimental population area revealed that four of the coyote/hybrid scats were detected within the home ranges of sterilized hybrids. The other coyote/hybrid scat indicated the presence of a previously unknown individual. We suggest this method be expanded to include more of the experimental population area and be optimized for use with nuclear markers to improve detection of hybrid and back-crossed individuals.

Animals↗
PubMed2000

Cardiorespiratory effects of four alpha2-adrenoceptor agonist-ketamine combinations in captive red wolves.

OBJECTIVE: To evaluate the cardiopulmonary effects of immobilizing doses of xylazine-ketamine (XK), medetomidine-ketamine (MK), medetomidine-ketamine-acepromazine (MKA), and medetomidine-butorphanol-ketamine (MBK) in captive red wolves. DESIGN: Prospective study. ANIMALS: 32 adult captive red wolves. PROCEDURE: Wolves were randomly assigned to 1 of 4 treatment groups: XK, MK, MKA, or MBK. Physiologic variables measured included heart rate, blood pressure, respiratory rate, tidal volume, oxygen-hemoglobin saturation (Spo2), end-tidal CO2, arterial blood gases, and rectal temperature. Induction time, muscle relaxation, and quality of recovery were assessed. RESULTS: Heart rates were lower in wolves in the MBK group than for the other groups. All 4 drug combinations induced considerable hypertension, with diastolic pressures exceeding 116 mm Hg. Blood pressure was lowest in wolves receiving the MBK combination. Respiratory rate was significantly higher in wolves receiving XK, MK, and MKA. Tidal volumes were similar for all groups. Wolves receiving XK, MK, and MKA were well-oxygenated throughout the procedure (SPo2 > 93%), whereas those receiving MBK were moderately hypoxemic (87% < Spo2 < 93%) during the first 20 minutes of the procedure. Hyperthermia was detected initially following induction in all groups. CONCLUSIONS AND CLINICAL RELEVANCE: The alpha2-adrenoceptor agonist-ketamine combinations provide rapid reversible anesthesia for red wolves but cause severe sustained hypertension. Such an adverse effect puts animals at risk for development of cerebral encephalopathy, retinal hemorrhage, pulmonary edema, and myocardial failure. Although the MBK combination offers some advantages over the others, it is advised that further protocol refinements be made to minimize risks associated with acute hypertension.

Acepromazine↗
PubMed2000

Shoulder muscle activation during aquatic and dry land exercises in nonimpaired subjects.

STUDY DESIGN: Randomized, single blind experimental design using electromyography to measure shoulder muscle activation in nonimpaired subjects. OBJECTIVES: To compare the muscle activation of rotator cuff and shoulder synergists during rehabilitation exercises performed in water or on dry land. BACKGROUND: Early motion is critical to restoration of normal shoulder function. Aquatic therapy has been promoted as a method for increasing range of motion while minimizing stress on the shoulder. METHODS AND MEASURES: The integrated electromyography amplitude of 6 muscles of the shoulder girdle was examined on the nondominant shoulders of 6 subjects (supraspinatus, infraspinatus, and subscapularis, anterior, middle, and posterior deltoids). Each subject performed elevation (0 degree to 90 degrees) in the scapular plane with neutral rotation on land and in water at 3 different speeds of elevation (30 degrees/s, 45 degrees/s, and 90 degrees/s). The mean percentage of the maximal voluntary contraction was determined for each of the 3 test speeds on land and in water. Comparisons between water and dry land were made with a repeated measures analysis of variance. RESULTS: For all 6 muscles tested, muscle activation during the 30 degrees/s test speed and all muscles tested at the 45 degrees/s test speed was significantly less when performed in water versus when performed on land. For example, electromyography activation of the supraspinatus muscle was 16.68% of a maximal voluntary contraction when elevation at was performed at 30 degrees/s on dry land versus 3.93% when performed in water. CONCLUSION: These data suggest that shoulder elevation in the water at slower speeds resulted in a significantly lower activation of the rotator cuff and synergistic muscles. This decreased muscle activation during aquatic physical therapy allows for earlier active motion in the postoperative period without compromising patient safety.

Adult↗
PubMed1997

Technical considerations for electromyographic research on the shoulder.

This study compared 2 methods of indwelling bipolar electrode insertion. One method used a single needle for the insertion of both wire leads, and the second method used 2 needles for the independent insertion of both leads at a specified interdetection distance. Simultaneous electromyography recordings from the 2 different electrode configurations were made during the activation of nonfatigued supraspinatus and infraspinatus muscles from 10 healthy subjects. Subjects performed 6 different isometric contractions at 3 different levels of force (100%, 60%, and 30% effort). In this study, the bipolar electrode configuration using 2 hypodermic needles for placement of the 2 leads produced superior electromyography recordings than did the configuration using a single needle for the wire lead insertion. The separated bipolar electrodes produced a significantly higher amplitude electromyography signal with less intersubject variability and greater partial correlation with force. This study suggests an alternative method of bipolar wire electrode placement that results in improved signal characteristics and decreased variability of signal acquisition. A standardization of wire electromyography examination of the shoulder that improves signal characteristics and acquisition ultimately will lead to more accurate results with greater clinical use and validity.

Action Potentials↗
PubMed1996

Optimal normalization tests for shoulder muscle activation: an electromyographic study.

To accurately compare electromyographic data from different muscles and different subjects, it is necessary to normalize the integrated data obtained from each muscle. The purpose of this study was to identify the manual muscle testing positions that elicit maximal neural activation (integrated electromyography) of three rotator cuff muscles (supraspinatus, infraspinatus, and subscapularis) and five shoulder synergists (pectoralis major, latissimus dorsi, and anterior, middle, and posterior deltoids). The electromyographic activity of these eight muscles was examined in the nondominant shoulders of nine subjects. Indwelling wire electrodes (supraspinatus, infraspinatus, and subscapularis) and surface adhesive electrodes (pectoralis major, latissimus dorsi, and anterior, middle, and posterior deltoids) were placed. Each subject performed a series of 27 isometric contractions, and optimal tests (maximal neural activation) were identified for each muscle. Four tests were identified that resulted in the maximal neural activation of all eight shoulder muscles: 90 degrees of scapular elevation with -45 degrees of humeral rotation for the supraspinatus, anterior deltoid, and middle deltoid: external rotation at 90 degrees of scapular elevation and -45 degrees of humeral rotation for the infraspinatus and posterior deltoid: internal rotation at 90 degrees of scapular elevation and neutral humeral rotation for the subscapularis and latissimus dorsi: and internal rotation at 0 degree of elevation and neutral rotation for the pectoralis major. These results identify four standard testing positions that will provide reference values for normalization of maximal voluntary contraction for the eight muscles of the shoulder examined in this study. Standardization of these test positions offers normalization guidelines that can be used in future dynamic electromyography studies of the shoulder.

Adult↗
PubMedDate not supplied

Function of the long head of the biceps at the shoulder: electromyographic analysis.

The purpose of this study was to delineate, through electromyographic analysis, the function of the long head of the biceps at the shoulder. Ten shoulders were examined with dynamic electromyography. The long head of the biceps was instrumented with thin wire electrodes. The supraspinatus, infraspinatus, deltoid, brachialis, and brachioradialis were instrumented as controls. Because the biceps functions primarily as a forearm supinator and elbow flexor, a long arm brace was used to lock the elbow in extension with the forearm in neutral pronation/supination. Each motion was tested in a full arc at fast (170 degrees per second) and slow (36 degrees per second) speeds and repeated with and without a 5-pound weight attached to the distal end of the brace. No electrical activity was identified in the long head of the biceps muscle in response to isolated shoulder motion with the elbow and forearm position controlled. The data demonstrate that the long head of the biceps is not active in isolated shoulder motion when the elbow and forearm are controlled. Thus, any hypothesis on bicipital function at the shoulder must be based on either a passive role of the tendon or tension in association with elbow and forearm activity.

Adult↗
PubMedDate not supplied

The manual muscle examination for rotator cuff strength. An electromyographic investigation.

The electromyographic activity of eight muscles of the rotator cuff and shoulder girdle (supraspinatus, infraspinatus, subscapularis, pectoralis, latissimus dorsi, and the anterior, middle, and posterior deltoid) was measured from the nondominant shoulders of 11 subjects during a series of 29 isometric contractions. The contractions simulated different positions used for strength testing of the rotator cuff and involved elevation, external rotation, and internal rotation at three degrees of initial humeral rotation (-45 degrees of internal rotation, 0 degree, +45 degrees of external rotation) and scapular elevation (0 degree, 45 degrees, 90 degrees). Isolation of the supraspinatus muscle was best achieved with the test position of elevation at 90 degrees of scapular elevation and +45 degrees (external rotation) of humeral rotation. Isolation of the infraspinatus muscle was best achieved with external rotation at 0 degree of scapular elevation and -45 degrees (internal rotation) of humeral rotation. Isolation of the subscapularis muscle was best achieved with the Gerber push-off test. This study used four criteria for identifying the optimal manual muscle test for each rotator cuff muscle: 1) maximal activation of the cuff muscle, 2) minimal contribution from involved shoulder synergists, 3) minimal provocation of pain, and 4) good test-retest reliability. Based on the results of this study and known painful arcs of motion, an objective identification of the optimal tests for the manual muscle testing of the cuff was elucidated.

Adult↗