Biomedical subjects

B Tank

Publications and source records attributed to B Tank.

40 records · Page 3Linked to original sources

PubMedDate not supplied

Absence of cytokeratin 8 and inconsistent expression of cytokeratins 7 and 19 in human basal cell carcinoma.

The expression of the low molecular weight cytokeratins (K) 7, 8, 18, 19 and the high molecular weight cytokeratin 10 in 21 basal cell carcinoma (BCC) was studied using seven different monoclonal antibodies (MoAbs) with specific anti-cytokeratin activity. MoAbs RCK 105 (anti-K7), RPN 1164 (anti-low molecular weight cytokeratins of basic group), Ks 19.1 (anti-K19) and Cam 5.2 (anti-K8, K18, K19) reacted positively but inconsistently in the BCC that were examined. MoAbs 1166 (anti-K8) and RGE53 (anti-K18) did not react at all. MoAb RKSE 60 (anti-K10) did not react with the tumor cells. From the results of this study, it can be concluded that cytokeratins 7 and 19 are expressed in BCC (43% and 71%, respectively), whereas cytokeratin 8 is not expressed.

Adult↗

PubMedDate not supplied

Immunoelectron microscopic studies on cytokeratins in human basal cell carcinoma.

Ultrastructural investigations into the location and the expression of the cytokeratins 7, 8, 10 and 19 were undertaken on ultrathin cryosections of 8 basal cell carcinomas (BCC) using a high resolution immunogold labeling method and five different monoclonal antibodies against specific cytokeratins. The results showed that cytokeratin 10 was expressed only in the differentiated keratinocytes. In contrast to the previously reported biochemical and immunohistochemical studies, in this study cytokeratin 19 was expressed not only in the tumor cells of BCC but also in the normal epidermal keratinocytes. The expression of cytokeratin 7 in BCC could not be confirmed but the lack of expression of cytokeratin 8 was confirmed, excluding its potential role as a specific histopathological marker for BCC.

Adult↗

PubMedDate not supplied

Expression of cytokeratin 8 and low molecular weight cytokeratins in human basal cell carcinoma.

The expression of low molecular weight cytokeratins (Cks) 7,8,18, 18 and high molecular weight cytokeratin 10 in 23 basal cell carcinomas (BCCs) was investigated using a panel of 14 different commercially available monoclonal antibodies (MoAbs) with specific anti-cytokeratin activity. Four of these MoAbs were directed against Ck 8. The results showed that Ck 8 was detected in all 23 BCCs using MoAb 4.1.18. Two of the MoAbs showed inconsistent staining for Ck 8 and one of them did not show any staining at all. Cytokeratins 7 and 19 were detected incosistently. Cytokeratins 18 and 10 were not detected in any of the 23 BCCs that were examined. The incosistent observations on the expression of Ck 8 in BCCs in this study could have been due to different epitopes of the different cytokeratins that were detected by the different MoAbs. The results of this study lead to recommendation that whenever possible a panel of different MoAbs directed against the same Ck(s) should be used in order to minimize the risk of obtaining incorrect experimental results.

Adult↗

PubMedDate not supplied

Expression of cytokeratin 8 in basal cell carcinoma: a comparative immunohistochemical and immunoelectron microscopy study.

The comparative study reported here was undertaken in order to resolve the discrepancies in the detection of cytokeratin (Ck) 8 reported in previous studies. The expression of Ck 8 was compared in 6 basal cell carcinomas (BCCs) using immunohistochemical and immunoelectron microscopic techniques and a panel of 4 different commercially available monoclonal antibodies (MoAbs). The results of this comparative study demonstrated not only that the consistent expression of Ck 8 using one of the MoAbs in immunohistochemistry was confirmed by immunoelectron microscopy, but that the inconsistent expression of Ck 8 observed using two other MoAbs was also confirmed. One of the MoAbs did not show any staining at all. The inability of this MoAb to detect the expression of Ck 8 using either of the techniques also indicated that this MoAb may be directed against an epitope of Ck8 that is not detectable in BCC in situ.

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