[Mechanisms of transcription in lymphocyte nuclei after antigenic stimulation in vitro].
Explore the source record for details and available documents.
Biomedical subjects
Publications and source records attributed to B Taudou.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
When guinea-pig lymph node cells were exposed to ConA in a culture medium lacking glutamine or cysteine, no DNA synthesis occurred. The addition of the missing acid to ConA-treated lymphocytes submitted to glutamine or cysteine starvation for 40 h allowed the synthesis of DNA to take place after a period of only 10-12 h. The synthesis of DNA is preceded by a rapid increase of 3H-uridine incorporation into RNA and of 3H-leucine incorporation into protein which occurred a few hours after addition of the missing amino acid. When cycloheximide was added to lymphocytes exposed to ConA in a glutamine or cysteine deprived medium, a relative enhancement of uridine incorporation was observed. No such effect was provoked by puromycin. These results suggest the possibility of a control system in lymphocytes similar to those described in microbial cells for amino acid control of RNA synthesis.