[Blastic transformation of the bone marrow preceded by extramedullary blastic transformation in chronic myeloid leukemia].
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Biomedical subjects
Publications and source records attributed to B Telek.
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Differential diagnostic importance of acid phosphatase and beta-glucuronidase reactions was studied in bone marrow smears of 52 patients with acute leukaemias. Both reactions showed either diffuse or simultaneously diffuse and granular positivity in the medullary blast cells of 34 patients suffering from ANLL. A strong diffuse positivity of acid phosphatase suggested the possibility of AMOL. Beta-glucuronidase and acid phosphatase reactions were exclusively granular in every positive case of ALL. Increased acid phosphatase activity was found in T-ALL while beta-glucuronidase showed increased activity also in (non-T, non-B)-ALL on several occasions.
The appearance of dual-marked (D) lymphocytes, i.e. lymphocytes having both T and B markers, was investigated in patients with acute (ALL) and chronic lymphocytic leukaemia (CLL) as well as with non-Hodgkin's lymphoma (NHL). E-rosette and immunobead-rosette techniques were combined and simultaneously administered, and the combination was found to be simple, fast and reproducible. D cells could be detected also in normal peripheral blood, however, only in a low percentage. In the blood of 4 patients suffering from ALL, in 2 with CLL and in 1 with NHL the number of D cells were increased significantly. In ALL the relative number of these cells decreased during remission and increased in relapses; thus the determination of the relative frequency of D cells might have prognostic value and could be useful in the choice of an optimal therapeutic protocol.
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Using qualitative and quantitative polarization microscopical techniques, an axiparallel orientation of chondroitin sulfate molecules and collagen fibrils could be detected in the osteocyte capsule of human embryonic and fetal bones. It is suggested that this spatially oriented microstructure plays a role in ana- and catabolic transport processes of the bone tissue.