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B Trevino

Publications and source records attributed to B Trevino.

4 recordsLinked to original sources
PubMed2002

Resistance training increases glucose uptake and transport in rat skeletal muscle.

The aim of this investigation was to determine if resistance training exercise improved glucose uptake and transport in rodent skeletal muscle. Sprague-Dawley rats were assigned to one of the three groups: control (CON), resistance trained (RT) and aerobic exercise trained (AT). Resistance trained rats were placed in a rodent squat apparatus and performed three sets of 10 repetitions at 75% of their one repetition maximum 3 days week-1 for 12 weeks. Aerobic exercise training consisted of running the rats 3 days week-1 for 45 min over a 12-week period on a motor-driven treadmill (32 m min-1, 15% grade). Following the training period, all animals were subjected to hind limb perfusion in the presence of 500 microU mL-1 insulin. Hind limb glucose uptake was similar in the RT (9.91 +/- 0.7 micromol g-1 h-1) and AT (10.23 +/- 1.0 micromol g-1 h-1) animals and significantly greater than control (CON) (6.40 +/- 0.6 micromol g-1 h-1). Rates of 3-O-methyl-d-glucose transport in the RT animals were elevated in the muscles utilized for RT while in the AT animals rates of 3-O-methyl-d-glucose transport were increased in those muscles recruited for running. The increased rates of 3-O-methyl-d-glucose transport in the skeletal muscles of the resistance trained and aerobic exercise trained animals appeared to be, in part, because of an increased GLUT4 protein concentration. These findings suggest that both resistance or aerobic training exercise can improve insulin-stimulated skeletal muscle glucose uptake and transport, but the training adaptations are restricted to the muscles recruited for the exercise performance.

3-O-Methylglucose↗
PubMed2001

Leptin administration improves skeletal muscle insulin responsiveness in diet-induced insulin-resistant rats.

In addition to suppressing appetite, leptin may also modulate insulin secretion and action. Leptin was administered here to insulin-resistant rats to determine its effects on secretagogue-stimulated insulin release, whole body glucose disposal, and insulin-stimulated skeletal muscle glucose uptake and transport. Male Wistar rats were fed either a normal (Con) or a high-fat (HF) diet for 3 or 6 mo. HF rats were then treated with either vehicle (HF), leptin (HF-Lep, 10 mg. kg(-1). day(-1) sc), or food restriction (HF-FR) for 12-15 days. Glucose tolerance and skeletal muscle glucose uptake and transport were significantly impaired in HF compared with Con. Whole body glucose tolerance and rates of insulin-stimulated skeletal muscle glucose uptake and transport in HF-Lep were similar to those of Con and greater than those of HF and HF-FR. The insulin secretory response to either glucose or tolbutamide (a pancreatic beta-cell secretagogue) was not significantly diminished in HF-Lep. Total and plasma membrane skeletal muscle GLUT-4 protein concentrations were similar in Con and HF-Lep and greater than those in HF and HF-FR. The findings suggest that chronic leptin administration reversed a high-fat diet-induced insulin-resistant state, without compromising insulin secretion.

3-O-Methylglucose↗
PubMedDate not supplied

A simple and rapid solvent extraction method for determining total lipids in fish tissue.

Solvent systems that have been developed for lipid extraction include chloroform-methanol, n-hexane-isopropyl alcohol, and methylene chloride-methanol. The extraction methods are labor intensive, lack precision, or require a large volume of solvent. Correct computation of lipid content calls for full recovery of solvent after extraction, but recovery always is incomplete because of unaccounted solvent residue that remains in jar, filter paper, and homogenized tissue. A rapid and simple extraction method coupled with correct computation was developed for determining total lipids in fish tissue. The method uses chloroform-methanol and an Eberbach blending jar. Variables examined were chloroform-methanol ratio, solvent-to-sample ratio, and phase separation time. Precision was within 0.5%. Conventional computation of lipid content depends on the volume of chloroform measured after filtration. This volume does not include unaccounted solvent residue. Thus, a time-consuming second extraction is required for complete recovery. The mass balance of each extraction and filtration step confirmed that the correct volume of chloroform (measured plus unaccounted) was close to the theoretical volume. The procedure eliminates problems associated with laborious filtration and variation in chloroform volume readings and does not require an exact reading of chloroform volume. Instead it allows use of a theoretical volume, which depends on solvent volume and ratio used.

Animals↗