PubMed Health⌕ Search

Biomedical subjects

B V Allen

Publications and source records attributed to B V Allen.

At least 19 recordsLinked to original sources
PubMed1988

Relationships between the erythrocyte sedimentation rate, plasma proteins and viscosity, and leucocyte counts in thoroughbred racehorses.

The influence of plasma proteins on erythrocyte aggregation was studied in a population of young thoroughbred racehorses, using the 60 minute erythrocyte sedimentation rate (ESR) with and without haematocrit standardisation. The ESR was correlated inversely with the haematocrit, but directly with fibrinogen, plasma viscosity and serum total globulins. When ESR values were standardised to a common haematocrit the correlation coefficients for the same plasma protein factors were increased. Albumin levels showed a strong direct relationship with haematocrit which accounted for the inverse correlation found between albumin and ESR. The haematocrit standardised ESR showed no significant correlation with albumin levels. Total leucocyte and absolute neutrophil counts were not correlated with either ESR or haematocrit standardised ESR. The high correlation (r = 0.75) found between fibrinogen levels and haematocrit standardised ESR suggests that differences in this acute phase protein influence the degree of red cell aggregation and rouleaux formation in the horse.

Animals↗
PubMed1986

Comparison of the haemogram between three-year-old Thoroughbred stayers and sprinters.

Results of blood counts have been analysed in three-year-old racehorses in training comprising 77 colt stayers, 27 colt sprinters, 61 filly stayers and 35 filly sprinters. The distributions of haemoglobin, erythrocyte count and haematocrit were significantly higher in colt stayers compared to the other three groups. In fillies these values were also significantly higher in stayers compared to sprinters. The erythrocyte count was significantly higher in filly stayers compared to colt sprinters but there were no significant differences between haemoglobin or haematocrit values in these groups. No significant differences were found in the erythrogram between colt and filly sprinters. Absolute lymphocyte counts were significantly higher in the two filly groups compared with the colts. Eosinophil counts were significantly higher in the stayer groups compared to the sprinters. The ranges of values observed, within each of the four groups, for haemoglobin, erythrocyte count and haematocrit were narrower than previously recorded.

Animals↗
PubMed1986

Effects of warfarin on blood rheology in navicular disease.

A preliminary investigation has been undertaken of blood rheology in horses and ponies, its variation in navicular disease and the changes following treatment with warfarin. Erythrocyte flexibility, measured by a centrifuge packing technique, is higher in horses (30 per cent min-1) than in ponies (23.8 per cent min-1). There are corresponding differences in blood viscosity. The high erythrocyte flexibility in horses is caused by an unknown factor present in plasma. The erythrocyte flexibility in horses with navicular disease is even higher, at 38.5 per cent min-1. Treatment with warfarin reduces the flexibility to just below the normal value. A significant fall in plasma viscosity and erythrocyte flexibility was found after treatment of four ponies with 6 mg warfarin daily for one week.

Animals↗
PubMed1984

Leucocyte counts in the healthy English Thoroughbred in training.

Total and differential leucocyte counts have been determined by electronic counting techniques in 474, two-to-four-year-old healthy Thoroughbreds in training. The ranges of values observed (particular those for neutrophils and lymphocytes) were narrower than previously described. Absolute and percentage lymphocyte values were significantly decreased with advancing age causing a relative increase in percentage neutrophils. The fall in absolute lymphocyte numbers was the main cause for a significant decrease in total leucocytes with age. Frequency plots for each variable showed that, with the exception of the eosinophil count, data from other leucocyte cell types following either a normal or lognormal distribution.

Aging↗
PubMed1984

Relationship between paired plasma and serum viscosity and plasma proteins in the horse.

The relationship between paired plasma and serum viscosity measurements and plasma proteins, including fibrinogen, were compared in 106 horses with both normal and abnormal serum protein levels. There is a highly significant positive correlation between serum viscosity and total serum proteins and total globulin levels. The difference between plasma and serum viscosity correlated well with clottable fibrinogen concentration. Albumin levels showed a negative correlation with plasma and serum viscosity, globulins and fibrinogen. Simultaneous estimation of serum and plasma viscosity improves the diagnostic value of the latter test without appreciable increase in cost or time and should prove useful for screening large numbers of samples for the presence or absence of abnormal levels of globulins and, or, fibrinogen.

Animals↗
PubMed1982

Some haematological data for fallow deer (Dama dama) in England.

The concentrations of haemoglobin, erythrocytes and leucocytes and the packed cell volume, plasma viscosity and some red cell indices were measured in the blood of 42 fallow deer and analysed with respect to age, season and sex. The mean haemoglobin concentration was greater in females than in males. In all the deer the number of leucocytes was considerably smaller than in the usual domestic mammals, namely, cats, cattle, dogs, goats, horses, pigs and sheep. Plasma viscosity was statistically greater in male deer more than two years old than in female deer of similar ages.

Animals↗
PubMed1981

Method for the automation of equine differential leucocyte counts.

A technique for automating equine differential leucocyte counts by analysis of volume distribution curves using the Coulter Channelyzer has been developed and evaluated. A comparison between the results obtained by this method and standard microscopic techniques showed good agreement in most cases. Blood samples can be analysed for both differential and total leucocyte counts at a rate of 25/h. For each sample an average 16,000 leucocytes are classified by the Channelyzer. The method of volume analysis is suitable for the precise counting of polymorphonuclear neutrophils, lymphocytes and eosinophils but blood films should still be screened to recognise any increase in monocytes or the presence of immature cells.

Animals↗
PubMed1978

Serum folate levels in horses, with particular reference to the English thoroughbred.

The assay of serum folate has been used to investigate the folate status of thoroughbreds in training to race, in-foal thoroughbred mares at stud and crossbred ponies kept permanently at grass. Throughbreds in training, with no obvious haematological or clinical abnormalities, had lower serum folate levels (mean 3.3, range 1.5 to 6.1 microgram per litre) compared with thoroughbred mares at stud (mean 10.6, range 6.4 to 15.8 microgram per litre) and crossbred ponies at grass (mean 10.9, range 7.4 to 16.6 microgram per litre. Intensive exercise, over a period of six months, significantly decreased serum folate levels (P less than 0.001) and significantly increased the levels of bilirubin (P less than 0.0001) and PCV (P less than 0.0002) in a group of 30 thoroughbred racehorses. Haptoglobin levels showed no significant changes.

Animal Feed↗
PubMed1978

A modified sedimentation method for counting platelets in blood.

A sedimentation method for counting human blood platelets is proposed which uses a fluid of sp.gr. i.057 at an osmolarity of 320 milliosmols. The fluid is a mixture of Lymphoprep and Isoton II. After centrifugation at about 200 platelets are counted on an electronic particle counter.

Blood Cell Count↗
PubMed1977

Suspension of non-viable fungal spores as a standard for platelet counting.

In the search among biological particles for a standard for counting human platelets, a strain of Absidia corymbifera was found to have spores that resembled platelets. After fixing in formalin and autoclaving the spores had a similar mean cell volume to that of human platelets. A suspension of these killed spores was tested in 100 laboratories and gave consistent results as a standard for human platelet counting. The Absidia corymbifera standard can be used in electronic counting methods but not in sedimentation methods, as the spores will be removed by centrifugation.

Blood Cell Count↗