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Biomedical subjects

B V Gushchin

Publications and source records attributed to B V Gushchin.

At least 19 recordsLinked to original sources
PubMed1991

[The lymphocyte acetylcholinesterase activity in rats poisoned by pesticides].

The experiments carried out present the evidence of acetylcholinesterase activity of Wistar rat lymphocytes. It was shown that splenocytes and thymocytes had significantly different levels of the enzyme activity. Peroral administration of phosphor-organic pesticide antio (phormothion) 1/100 and 1/20 LD50 induced the dose-dependent inhibition of splenocyte acetylcholine-esterase activity after 2 months of treatment. It suggests the relation of the immunosuppressive action of pesticide with the interference into the neuromediator mechanisms regulating the lymphoid cell function.

Acetylcholinesterase↗
PubMed1977

[Transfection by integrated proviruses].

Three lines of continuous mouse L cells were compared: one of them contained only its endogenic oncornavirus, another was contaminated with SV5 virus, and a third was obtained from the second line chronically infected with vesicular stomatitis virus. Experiments with transfection showed that it was possible to recover SV5 virus from the two former cultures and vesicular stomatitis virus from the latter.

Animals↗
PubMed1977

[Study of the morphological aspects of Okhotskiĭ virus replication in a chick fibroblast culture].

Electron microscopic studies of chick embryo fibroblast cells 24 hours after infection with Okhotsky virus revealed changes typical orbivirus infection in the cytoplasm and the nuclei of the cells. The nuclei of the infected cells contained fine-granular matrices with forming virus particles and tubular structures. The cell cytoplasm also contained tubular structures, crystal arrays, fine-granular matrices and virus particles 50-60 nm in diameter.

Animals↗
PubMed1976

Persistent SV5 virus infection in continuous cell cultures.

A continuous line of guinea pig kidney cells (CGPK/H) and a continuous line of mouse fibroblasts (L/H) spontaneously infected with parainfluenza virus SV5 were found. These cultures showed no enhanced cell degeneration or symplast formation, nor was haemagglutinin accumulation or infectious virus demonstrated in them. However, regular reproduction of ribonucleoprotein (RNP) characteristic of parainfluenza viruses, morphologically complete virions and antigens producing antibody to SV5 virus were found in the cells. Focal haemadsorption neutralized by antiserum to SV5 virus was also demonstrated. The infection persisted in the cell populations for over 2 years (the observation period) under standard conditions of cell dispersion and subcultivation.

Animals↗
PubMed1975

[Transformational activity of the oncornaviruses isolated from RH and Hep-2 cell lines].

Oncornaviruses isolated from transplantable cell cultures Hep-2 and RH were used for inoculating primary tripsinized cell cultures of human foetal kidney (HFK) and human embryonic fibroblasts (HEF). In all 15 cases no transformation of HEF cells was noted. In inoculation of HFK with oncornaviruses isolated from RH cell culture in 2 of 16 cases there were obtained transformed cell cultures: HFK+VRH and HFK+VRH Mc) occurred twice as fast. The transformed cell cultures have gone through 15-23 passages and were characterized by a high mitotic activity, production of oncornaviruses, type A and B, and absence of contact inhibition, a capacity for multistratum three-dimensional growth, the modal number of chromosomes 64 and a mixed type AB of electrophoretic mobility of glucoso-6-phosphate dehydrogenase.

Carcinoma, Squamous Cell↗
PubMed1975

[A comparative study of primary human cell cultures of diffuse goiter and cancer of the thyroid gland].

Cells obtained from non-malignified tissues (diffuse struma) in 83 of 100 cases formed a continuous layer consisting of monomorphous epithelioid cells possessing a high adhesive capacity. Cells obtained from thyroid adenocarcinoma (in 5 of 7 cases) showed lowered adhesiveness and did not form a monolayer. The culture consisted of polymorphous cells. Gigantic multinuclear cells and these with spheroid colorless inclusions in nuclei were encountered. In tripsinization of the tumor tissue obtained from patients, subjected to x-ray therapy, no growth of cell cultures was detected. Electron microscopic studies of nonradiated tumor tissue revealed light and dark cells, while these were light and degenerating in the irradiated tissue.

Adenocarcinoma↗
PubMedDate not supplied

[Electron microscopic studies of cell cultures of various origins].

Thirty-four cell cultures of various origins were examined in the electron microscope. Mycoplasma were found in 31 cultures, A, C and D particles of oncornaviruses in 27 cultures, adenovirus in one culture and paramyxovirus in 8 cultures. Two cultures were found to be virus- and mycoplasma-free. A stringent control of cell cultures for the presence of viruses, mycoplasma or other microbiological agents is recommended.

Adenoviridae↗
PubMedDate not supplied

[Electron microscopic study of chicken fibroblasts infected with the Tyuleniy virus (author's transl)].

Uninfected and Tyuleniy virus-infected chick fibroblast cells were examined. Intracytoplasmic type A particles and immature type C particles of oncornavirus were found in uninfected cells. At 48 and 72 hours after inoculation of the cells with Tyuleniy virus a large number of mature C particles and virions of Tyuleniy virus were found in the cell cytoplasm. Possible relationships between A and C particles are discussed and a stimulating effect of Tyuleniy virus on mass maturation of C particles and their release from the cell is suggested.

Animals↗
PubMedDate not supplied

[Identification of a hemadsorbing agent discovered in uninfected mouse L cell cultures and also the same cultures chronically infected with Sindbis virus].

Electron microscope examinations of continuous lines of mouse L cells, both uninfected (L-init) and chronically infected with Sindbis virus (L-SV) revealed accumulations of ribonucleoprotein strands and virions corresponding by their parameters to paramyxoviruses in the cytoplasms of the cells. Further studies showed L-init and L-SV cell lines to have a manifest hemadsorption effect which could be completely inhibited by antiserum to parainfluenza SV5 virus. Immunofluorescence procedures detected intensive fluorescence in the cytoplasm of these cells which was observed only after treatment of the cells with antiserum to SV5 virus. In response to inoculation of cell homogenates of continuous L-init and L-SV cultures guinea pigs developed antihemagglutinating antibody to simian parainfluenza SV5 virus. On the basis of these results it may be assumed that virus-specific structures and viruses identical by their parameters to paramyxoviruses observed in electron microscope examinations of continuous mouse L-init and L-SV cells are simian parainfluenza SV5 virus.

Animals↗
PubMedDate not supplied

[Interaction of Sindbis virus with a culture of cells producing oncornavirus].

Electron microscopy and biophysical methods were used for examinations of primarily trypsinized chick fibroblast cell culture spontaneously producing C-type oncornavirus at 1, 6, and 24 hours after inoculation with Sindbis virus. During the first 6 hours rapid maturation and release of oncornavirus from cells were observed. At later intervals oncornavirus production was inhibited. It is assumed that in the system under study, biosynthesis of oncornavirus and Sindbis virus occur separately.

Animals↗