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B V Moroz

Publications and source records attributed to B V Moroz.

15 recordsLinked to original sources
PubMed1990

[The use of commercial immunoenzyme kits for the diagnosis of toxoplasmosis].

The results of comparative study of sera obtained from donors and from several groups of patients with suspected toxoplasmosis are presented. The study has been carried out with the use of commercial enzyme immunoassay (EIA) kits: Toxoplasma gondii IgG EIA and Toxoplasma gondii IgM EIA manufactured by Labsystems (Finland), Sevatest ELISA IgG Toxo Micro I manufactured by Sevac (Czechoslovakia). Statistical processing of the results has confirmed the identity of these kits. The necessity of using evaluation criteria (the separation point, the scale for the interpretation of results) when working with the Sevac kits is emphasized. Comparative evaluation of antibody profiles in the sera under test suggests that the titer less than 1:1600 should be regarded as the separation point for these kits. IgM antibodies to T. gondii have been found only in 22% of patients with high titers of IgG antibodies.

Animals↗
PubMed1988

[Recording and interpretation of the results obtained by using the ELISA Sevatest commercial immunoenzyme kit for detecting Toxoplasma gondii antibodies].

The results of the repeated tests of 295 serum samples from patients, previously examined by means of enzyme immunoassay screening and traditional serological tests (complement fixation, indirect immunofluorescence, and indirect hemagglutination) and 115 serum samples from healthy donors, studied by enzyme immunoassay techniques with the use of the commercial kits Sevatest ELISA, have been analyzed. The methodological approach permitting the determination of the final titer of the serum under study, taken in a dilution of 1:800, by its optical density has been used. The mean geometric titer for the control group of donors, determined in the enzyme immunoassay, has been 1:800. This fact suggests that titers exceeding 1:1,600 should be considered diagnostically significant.

Animals↗
PubMed1987

[Modern methods in the serological diagnosis of toxoplasmosis].

A total of 235 sera from patients suspected for toxoplasmosis were examined in the enzyme immunoassay (EIA), complement fixation, indirect immunofluorescence (IIF) and indirect hemagglutination (IHA) tests carried out in parallel. Good correlation between all tests was noted. As confirmed by the data obtained in this investigation, EIA showed the highest sensitivity. In IIF and IHA tests the maximum titers (1:512) were obtained in 2% of cases, while in EIA 17.5% of sera were positive in titers of 1:800 to 1:25,000. High sensitivity and good reproducibility of this assay suggest the necessity for its standardization and automation, which will permit its further use in laboratories at any level.

Complement Fixation Tests↗
PubMed1987

[Comparative evaluation of the commercial Sevatest ELISA immunoenzyme set and other serological tests for detecting Toxoplasma gondii antibodies].

This work analyzes the results of 4 serologic tests used for the diagnosis of toxoplasmosis: the complement fixation (CFT), indirect immunofluorescence (IIF), passive hemagglutination (PHAT) tests, and the enzyme-linked immunosorbent assay (ELISA). The last-mentioned one was made with the use of the commercial kits Sevatest ELISA IgG/Toxo Micro I. The results of ELISA were in good correlation with those yielded by the traditional tests: 70% coincidence with CFT, 80% with IIF, 84% with PHAT; besides, ELISA has shown a higher sensitivity in the screening of sera.

Antibodies↗