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Biomedical subjects

B V Pinegin

Publications and source records attributed to B V Pinegin.

At least 127 records · Page 7Linked to original sources
PubMedDate not supplied

The occurrence of natural antibodies to minimal component of bacterial cell wall (N-acetylglucosaminyl-N-acetylmuramyl dipeptide) in sera from healthy humans.

ELISA assay showed that sera from each of 729 healthy donors contained antibodies to the minimal component of bacterial cell walls, N-acetylglucosaminyl-N-acetylmuramyl dipeptide (GMDP). Anti-GMDP antibody levels were determined for 686 sera which were classified into 3 groups: high (17.6%), medium (68.7%), and low responder (13.7%). Inhibition analysis performed on representative sera showed that a proportion contained specific anti-GMDP antibodies reacting only with GMDP (i.e., GMDP interaction with anti-GMDP antibodies was inhibited by GMDP only) whereas the remaining sera reacted both with GMDP and with the tetrasaccharide (GlcNAc-MurNAc)2 (i.e., GMDP interaction with anti-GMDP antibodies in the latter sera was inhibited by both GMDP and the tetrasaccharide). Inhibition analysis indicated, moreover, that the anti-GMDP antibodies contained in high-responder sera had higher affinity than those present in low-responder ones: GMDP inhibited the GMDP + anti-GMDP antibody interaction by 88.7% in the former sera vs. 53% in the latter. Sera contained both IgM and IgG antibodies to GDMP, but the mean level of anti-GMDP IgG antibodies in the high-responder sera was 30 times higher than in the low-responder ones.

Acetylmuramyl-Alanyl-Isoglutamine↗
PubMedDate not supplied

[The use of biological preparations for correction of microbial status in pregnant women].

15 pregnant women with pregnancy lasting 28-32 weeks, whose medico-laboratory data (the positive result of the amino test, high pH value and the detection of "key" cells) suggested the presence of bacterial vaginosis, were placed under observation. The bacteriological study of vaginal microflora in all these women revealed pronounced disturbances simultaneously with the development of intestinal dysbacteriosis. The local application of the bacterial preparation "Zhlemik" containing freeze-dried live lactic acid bacteria of vaginal origin and the oral administration of lactic acid bifidumbacterin containing live bifidobacteria of intestinal origin permitted the successful restoration of vaginal and intestinal microbiocenosis. The capacity of biotherapeutic preparations for inducing non-specific immunostimulation led to a significant rise in the levels of IgA, IgM and IgG in vaginal secretions.

Bacteriocins↗
PubMedDate not supplied

[Flow laser cytometry for evaluation of the human immune system].

Some flow laser cytometry (FLC) techniques intended for studies of the immune system cells are reviewed. A widespread analytical method is the phenotyping of lymphocytes by the markers they express. The use of FLC permits the evaluation of practically all functional parameters of immunocompetent cells. Thus, to analyze their ingestive and microbicidal activity fluorochrome-labeled microorganisms are used. The apploication of indicator dyes makes it possible to evaluate calcium mobilization and formation of active forms of oxygen. FLC is used for the identification of cytokines inside the cell and in the medium. The authors propose tests for the analysis of the proliferative activity of lymphocytes, the cytotoxicity of natural killers, the evaluation of apoptosis and protein processing with monocytes/macrophages.

Apoptosis↗
PubMedDate not supplied

[Multiple cerebral abscess caused by Nocardia brasilientis].

Streptococcus aureus, streptococci, and enterobacteria were major causative agents of abscesses of the brain. At present, associated infection is responsible for 60% of cerebral abscesses. When systemic and specific immunities are diminished, a risk for cerebral abscesses caused by fungi increases. Among them, there are the most common fungi Candida species or Aspergillus species. The paper gives an example of successful complex treatment of multiple cerebral abscesses caused by an association of Nocardia brasiliensis and Staphylococcus epidermidis. The specific feature of the observation was the correct diagnosis before intraoperative verification of cerebral abscesses. The disease developed in the presence of diminished systemic immunity and in the absence of specific immunity to Nocardia. Immunodeficiency could be caused by prior herpetic infection, Epstein-Barr disease, a wasp bite. The pathogen that was able to cause microstrokes played an indubitable role in the pathogenesis of the disease, which predisposed to the development of cerebral abscesses in patients with immunodeficiency. Impaired blood circulation in the occipital region could be also induced by wasp venom.

Brain Abscess↗
PubMedDate not supplied

[Effect of vinblastine and cyclophosphamide on the formation of antigen-induced B-suppressors].

Seven days after administration of sheep red blood cells (SRBC) to CBA and (CBA X C57BL/6) F1 mice antigen-induced B-suppressors are formed in the spleen. These suppressors are capable of inhibiting the unirradiated syngeneic recipients' response to SRBC. Administration to donors to vinblastine (1.5 mg/kg) concurrently with SRBC did not affect and partially reversed, 5 days after SRBC, the formation of antigen-induced suppressors. On the contrary, administration to donors of cyclophosphamide (20 mg/kg) 24 h prior to SRBC or in a dose of 250 mg/kg concurrently with SRBC or 5 days after SRBC completely reversed the formation of antigen-induced suppressors.

Animals↗
PubMedDate not supplied

[Assessing the basic stages of the phagocytic process: current approaches to and prospects for research development].

The paper combines dedications to the marked event of Russian biology and medicine, I. I. Mechnikov's 150th birthday, and to the development of one of the most important problems of modern experimental medicine, the application of advances made in the study of phagocytosis to clinical practice. Based on the data available in the literature and their own findings, approaches to a stepwise assessment of phagocytosis is substantiated. A special attention is given to the discussion of the clinically available techniques which adequately evaluate phagocytic adhesion, degranulation processes, oxygen metabolic activation giving rise to active forms of oxygen, nitrogen, the killing and decomposition of phagocytotic objects. The material is presented by aiming at the tasks of clinical medicine to diagnose the major phagocytotic disorders within the general examination of immunity functions.

Evaluation Studies as Topic↗