Modelling of the crystallization kinetics of cocoa butter.
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Cacao↗
Biomedical subjects
B Vanhoutte
Publications and source records attributed to B Vanhoutte.
The effect of phospholipids and water on the isothermal crystallisation behaviour of milk fat.
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Animals↗
Combined liquid and gas chromatographic characterisation of polyglycerol fatty acid esters.
In the present study a combined liquid and gas chromatographic technique is described for the analysis of polyglycerol fatty acid esters. Liquid chromatographic fractionation of samples resulted in pure standards of monoesters of di- and triglycerols and diesters of di- and triglycerols. Confirmation of their identity was achieved by LC-MS analysis. Moreover, a chromatographic identification of the mono- and diesters of cyclic diglycerol was proposed. From the isolation of pure esters and their gas chromatographic analysis, it was revealed that co-elution of several compounds occurred. Thus it was shown that prefractionation of the sample using a simplified liquid chromatographic separation, was necessary in order to characterise the esters correctly. In combination with some other chemical analyses, a complete profile of the chemical composition of polyglycerol fatty acid esters can be obtained.
Chromatography, Gas↗
Physiological investigations by image analysis.
Modern quantitative image analysis has been extensively used to characterize the morphology of microorganisms, especially those of the filamentous type. More recently physiological features have been quantified, making use of classical stains as well as fluorescent dyes. The potential of the technique is illustrated by a detailed analysis of the differentiation of Streptomyces ambofaciens. Three different staining procedures have been used to monitor the thinning and septation of hyphae (with propidium iodide staining), the leakage of cellular components through the membrane (with carbol gentian violet staining) and the respiration (with INT staining) in a batch submerged culture.
Gentian Violet↗