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B Visseaux-Coletto

Publications and source records attributed to B Visseaux-Coletto.

10 recordsLinked to original sources
PubMed2000

Image cytometry detection of breast cancer cells with > 5C DNA content and minor DNA stemlines.

OBJECTIVE: To determine if the presence of cells having a DNA content > 5c and occurring at very low frequency is related to breast cancer outcome. STUDY DESIGN: Feulgen-stained imprints of fresh tumors used for routine standard DNA image cytometry were reanalyzed, with the aim of detecting hyperploid (> 5c) cells or minor stemlines. Specially adapted software was used. RESULTS: The new DNA analysis showed discordance of 47.3% with standard DNA cytometry. Minor stemline or rarely occurring 5c exceeding cells were found. These were not detected by the first DNA analysis. The presence of both DNA hyperploid cells occurring as rare events and a DNA hyperploid stemline was related to outcome. CONCLUSION: The detection of DNA hyperploid cells, even in very small numbers, appears essential to outcome, particularly in diploid or single DNA aneuploid breast cancers.

Adenocarcinoma, Mucinous↗
PubMed1995

Determination of reliable histological features associated with early triploidy using DNA image cytometry.

In order to determine reliable histological features characterizing triploidy, the following features were examined and graded by three pathologists on 46 early abortion specimens: hydropic swelling of the villi, cisterns, villous scalloping, trophoblastic hyperplasia with syncytial vacuolization, single cytotrophoblastic cells in villous stroma, trophoblastic inclusions, microcalcifications and fibrosis. At the same time, the DNA content of the 46 specimens was quantified cytophotometrically using the CAS 200 image analyzer, in order to confirm or not the diagnosis of triploidy. Triploidy was confirmed in 45.7%, and in the triploid group, the grading of three features differed significantly from the non triploid group i.e. cisterns (p < 0.001), trophoblastic hyperplasia (p < 0.05) and trophoblastic inclusions (p < 0.05). Using these three features we were able to give a triploid score per slide which represents the sum of the grades of each of these three features. The minimum score obtained was 0, the maximum was 6. The average of these scores in the triploid group was 3.095, and 1.45 in the non triploid group (p < 0.001). This scoring method on the three significant features (cisterns, trophoblastic hyperplasia and trophoblastic inclusions) appears to be useful to classify an abortion specimen as triploid or not, and to select the specimens for which a DNA quantification may be necessary.

Abortion, Spontaneous↗
PubMed1994

[Quantification of DNA using image analysis on paraffin sections].

With the new softwares that are available, it is now possible to measure the cellular DNA content on Feulgen stained paraffin sections, without enzymatic dissociation of the samples. The main advantage of this technique is to preserve the cellular histological context and to select the right cells that have to be analyzed. The application is of course to obtain information about cancer prognosis, but it can also be very useful in non tumoral pathology. The DNA measurement on paraffin sections can be applied to the diagnosis of triploidy or tetraploïdy in spontaneous abortions without any karyotype.

DNA↗
PubMed1993

Discrepancies of DNA content of various solid tumours before and after culture measured by image analysis. Comparison of cytogenetical data.

Parallel cytophotometric ploidy studies and cytogenetic analysis were performed on 15 various human solid tumours. The quantification of DNA by image analysis was carried out on cytological imprints of fresh tumours and on smears obtained after cell culture. The results obtained by both sets of calculations were compared with each other and with the cytogenetic results. 6 cases (40%) showed concordance between the 3 techniques. One case was aneuploid for both DNA image analysis measurements but the cytogenetic data showed only a diploid stem line. In 3 cases out of 15 (20%), smears DNA analysis and cytogenetic results were concordant: in 2 tumours, the culture step failed to preserve aneuploid stem lines that were present in the imprint analysis. In the third one, a minority tetraploid peak observed after culture was absent on the imprint slide. Concordance between imprints and cytogenetic data and discordance with smears' analysis was observed in 3 cases (20%). These 3 cases were diploid or near diploid but the DNA analysis on the smears after culture showed an aneuploid stem line in each case. The last 2 cases showed a total disagreement between the 3 techniques. By measuring the DNA content with an image analyser, the observer can ensure that only tumoral cells are taken into account. The present study revealed that cytogenetic data represent only about 60% of the population that is effectively present in the culture dish and that the cultured population represents only 47% of the population present on the fresh tumour imprint.

Cells, Cultured↗
PubMed1993

[Cell proliferation in tumor pathology].

There are many ways to analyse and quantify the proliferating activity of a tumor. The different technics are more and more specific of each phase of the cell cycle. This paper firstly describes the basis of each of these technics (mitosis count, DNA staining with various compounds fluorescent or not, incorporation of specific molecules during the phase of DNA synthesis, detection of proliferation antigens by immunological methods). Secondly, the interest of the proliferating fraction knowledge of a tumor is discussed, for various pathologies and according to the technic used.

Cell Cycle↗
PubMed1992

Semiautomatic quantification of silver-stained nucleolar organizer regions in tissue sections and cellular smears.

Silver staining of nucleoli reveals argyrophilic proteins associated with nucleolar organizer region (Ag-NOR) proteins. Argyrophilic components appear as dots about 1 micron in diameter dispersed throughout the nucleolus (Ag-NOR dots). The count of Ag-NOR dots is a useful index for improving the cancer diagnosis and determination of prognosis. Here we describe software developed on a medium-cost image analyzer in order to evaluate the mean area of NORs and their number relative to an internal reference, the number and areas of clusters of NORs and the area of the nucleus. Statistical analysis of the data was performed during counting. The first application concerned counting NOR dots during mitosis in cell imprints; those counts were 2.3, 15.3 and 55.56 for the metaphase, telophase and interphase, respectively (relative to unitary dots of metaphase cells). In the second application we demonstrated a significant difference in NOR numbers between two groups of prostatic cancers with good and poor prognoses (6.05 +/- 2.79 SD and 7.96 +/- 3.01, respectively; with Student's t test, = 1.999; P = .05).

Animals↗
PubMed1991

[Nucleolar organizers in superficial bladder tumors. Prognostic analysis of 37 observations].

Nucleolar Organisers (NORs) are intranucleolar segments of DNA coding for ribosomal RNA. The agyrophilic proteins (AgNOR) associated with NORs allow them to be cytolabelled on paraffin sections. The number of NORs (NOR index) is correlated with cellular proliferation and has a diagnostic and prognostic value in neoplastic disease. The AgNOR method was analysed in a series of 37 superficial bladder tumours with different clinical courses. The NORs were counted in normal urothelium, on superficial tumours used to establish the diagnosis of the disease and on recurrent superficial tumours. The NOR index was 4.54 for normal urothelium, 5.89 for non-invasive superficial tumours, 7.33 for invasive superficial tumours, and 9.75 for invasive recurrences. These results demonstrate an increase in nucleolar argyrophilia with invasion and invasive potential of superficial bladder tumours which were initially homogeneous for stage and grade. The AfNOR method could constitute a new method of early histoprognostic evaluation for urothelial tumours.

Actuarial Analysis↗
PubMed1986

[Anaplastic small-cell bronchial carcinoma of the composite type].

The authors report an anatomico-clinical case (with ultrastructural study) of bronchial anaplastic carcinoma with composite, triple differentiation oat-cell subtype. The patient underwent early surgical excision followed by radiotherapy and chemotherapy. With the help of data from the literature concerning this composite subtype which suggests new theories on the histogenesis of bronchial cancer, the authors emphasize the value of surgical treatment for this unusual subtype of anaplastic carcinoma.

Aged↗
PubMed1982

[Colonic nerve network demonstrated by quinacrine].

Quinacrine can be used for fluorescence microscopy to visualize certain cell bodies and fibres in the myenteric plexus of the intestine, forming a network different from the cholinergic and adrenergic systems. In the Piebald mouse, animal model of congenital megacolon, the quinacrine positive plexus is thin in the distal narrowed segment and well developed in the large proximal zone. In Hirschsprung's disease, the quniacrine positive network has the same pathologic features but, contrary to the animal model, there is an increased number and size of extrinsic cholinergic and adrenergic fibres. So this mouse is an animal model of a pseudo-Hirschsprung's disease, which has not yet been reported in human pathology.

Adenosine Triphosphatases↗