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B Visser

Publications and source records attributed to B Visser.

23 records · Page 2Linked to original sources
PubMed1989

A screening for the presence of four different crystal protein gene types in 25 Bacillus thuringiensis strains.

25 different strains of Bacillus thuringiensis, belonging to 18 different serotypes were screened by Southern hybridization analysis for the presence of nucleotide sequences of four different gene types coding for crystal proteins showing different insecticidal spectra. Many but not all strains showed sequences homologous to any of the probes used. Homology with sequences of the gene type present in strain kurstaki HD1 occurred in all positive strains, whereas the other gene types were much less abundant. Sequences homologous to those of gene type VI, coding for a Spodoptera-specific crystal protein, appeared only in strains of serotypes aizawai and entomocidus.

Bacillus thuringiensis↗
PubMed1988

Congenital hemihypertrophy associated with posterior interosseous nerve entrapment.

An entrapment of the posterior interosseous nerve (PIN) associated with congenital hemihypertrophy has not been reported previously. A 63-year-old man noted that since birth his right arm had been considerably larger than his left. Fifteen years before our initial examination, however, he first noted weakness of his right wrist and finger extensors without sensory loss. Physical examination revealed atrophy and weakness of the musculature innervated by the PIN distal to the supinator and the first dorsal interosseous (FDI). Sensation was intact throughout the entire right arm. Electromyographic examination demonstrated membrane instability and decreased activation of voluntary motor units in the radial nerve musculature distal to the supinator and in the FDI. Electrophysiologic evidence suggested an anomalous innervation of the FDI by the PIN. Surgical exploration revealed hypertrophy of the brachioradialis, extensor carpi radialis longus and brevis, and supinator muscles, with atrophy of all muscles distal to the supinator. The supinator muscle appeared to be constricting the PIN and was sectioned. Clinical and electrodiagnostic follow-up at six months and again at four years revealed no further deterioration.

Arm↗
PubMed1985

Molecular cloning and the nucleotide sequence of the Mr 28 000 crystal protein gene of Bacillus thuringiensis subsp. israelensis.

The Mr 28.000 crystal protein gene of Bacillus thuringiensis subspecies israelensis has been cloned into pBR322 as part of a 9.7 kb HindIII fragment. From hybridization experiments of recombinant p425 DNA with B.t. subspecies israelensis RNA from different stages of growth it was concluded that transcription of the gene is restricted to early sporulation stages. Nucleotide sequence analysis revealed the presence of a large open reading frame with a coding capacity of 249 amino acids (Mr 27.340). Nuclease S1 mapping demonstrated that transcription starts 44 nucleotides upstream of the initiation codon. A Shine-Dalgarno sequence (AAGGAG) was found 10 nucleotides upstream of the translation startpoint. At the 3'-end of the gene a complex secondary structure was found immediately after the stop-codon. Despite the presence of these regulation signals only limited expression in E. coli was detected. This can be explained by assuming that B.t. subsp. israelensis promotor sequences are poorly recognized by E. coli RNA polymerase.

Amino Acid Sequence↗