Biomedical subjects
Publications and source records attributed to B W Hodgson.
A simplified latex bead immunoagglutination test is described employing the use of commercial antibody. It is practicable, reproducible and valid in screening for 'microalbuminuria' in diabetic patients. Although it is recommended as a side-room test its use should be supplemented where indicated by laboratory-based measurements.
Over the last 15 years, endogenous spleen-colony formation in our mice, following lethal irradiation, has increased to an unacceptable level. It has been found necessary, therefore, to introduce a new method of preparing recipient mice for spleen-colony studies. Irradiation with low dose-rate 60Cobalt gamma rays has been compared with high dose-rate linear accelerator electrons, and their effects on endogenous spleen colony formation compared with earlier X and gamma ray dose-response data. It was found that a large dose (13.5 Gy) of gamma rays results in fewer endogenous colonies than 8.5 Gy of electrons, yet because of its low dose rate (14.1 X 10(-3) Gy/min) it has a marked sparing of the intestinal tissue as measured by the intestinal microcolony technique. This in turn permits better survival and, therefore, a 'healthier' animal for spleen-colony work. Exogenous colony formation is also lower in the low dose-rate, gamma-irradiated recipients and this is shown to be due to a reduced spleen-seeding efficiency. It is concluded that very low dose-rate radiation is preferable for haemopoietic ablation, that a mouse colony requires constant monitoring for changes of endogenous spleen-colony formation and that the spleen-seeding efficiency of CFU-s depends on the irradiation technique used--there is no absolute value for a given strain of mouse.
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The effect of mitogens on the degree of polarization of the fluorescence from viable human lymphocytes, labeled with fluorescein, have been reported to be different in cells from subjects with malignant disease as compared with those from healthy donors. This has potential applications in the field of clinical diagnosis and a flow cytometer could be the instrument of choice for this purpose. We believe that such an instrument should be simple and easy to use and a flow cytometer was assembled with this in mind and with the object of gaining experience which would provide the basis for a future finalized design. The instrument is suitable for use in other biologic applications where polarization is of interest and also as a general purpose flow cytometer. Its main features, its characteristics and preliminary performance report are presented here.
An interface between a commercial flow cytometer (Ortho Cytofluorograf 4800) and a Desk Top Computer (H.P. 9845S) is described. Programs for data acquisition and analysis have been developed. These include cell DNA content analysis, 2-parameter (3D) histograms and sophisticated sub-population selection. Although cell sorting is not possible using the facility, the data acquired per cell is optimised and detailed analysis can be carried out. The system represents a flexible, easily controlled and economical method for storing and analysing flow cytometric data.
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Explore the source record for details and available documents.
Explore the source record for details and available documents.