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Biomedical subjects

B W Hudson

Publications and source records attributed to B W Hudson.

At least 19 recordsLinked to original sources
PubMed1981

Evaluation of the solid-phase radioimmunoassay for diagnosis of St. Louis encephalitis infection in humans.

Since the use of highly purified viral proteins in the solid-phase radioimmunoassay (SPRIA) procedure is not practical for routine diagnostic serology, the procedure was adapted for use with the crude antigens now commonly used to diagnose St. Louis encephalitis (SLE) virus infections. SLE and various other alpha- and flavivirus antigens were tested by both the SPRIA procedure and conventional serological methods. Standards were established for use of the crude antigens in the SPRIA procedure. Reproducibility of the procedure was similar to that of conventional tests. The SPRIA procedure specifically differentiated SLE from clinically similar alphaviruses, but did not differentiate SLE from related flaviviruses very well. SPRIA diagnosis of SLE infections by the immunoglobulin G response in paired sera was good. Generally, the crude antigen SPRIA procedure was as sensitive as the conventional serological tests, but not as specific.

Antibodies, Viral↗
PubMed1980

Plague in Central Java, Indonesia.

Plague in man occurred from 1968 to 1970 in mountain villages of the Boyolali Regency in Central Java. Infected fleas, infected rats, and seropositive rats were collected in villages with human plague cases. Subsequent isolations of Yersinia pestis and seropositive rodents, detected during investigations of rodent plague undertaken by the Government of Indonesia and the WHO, attested to the persistence of plague in the region from 1972 to 1974.Since 1968, the incidence of both rodent and human plague has been greatest from December to May at elevations over 1000 m. Isolations of Y. pestis were obtained from the fleas Xenopsylla cheopis and Stivalius cognatus and the rats Rattus rattus diardii and R. exulans ephippium. The major risk to man has been fleas infected with Y. pestis of unique electrophoretic phenotype. Infected fleas were collected most often in houses.Introduced in 1920, rodent plague had persisted in the Boyolali Regency for at least 54 years. The recent data support specific requirements for continued plague surveillance.

Animals↗
PubMed1979

Delayed-type hypersensitivity responses in mice infected with St. Louis encephalitis virus: kinetics of the response and effects of immunoregulatory agents.

Labeled monocyte infiltration techniques have been used to study delayed-type hypersensitivity responses in mice immunized with St. Louis encephalitis virus. A delayed 24- to 48-h inflammatory response occurred 6 to 7 days after immunization. This response can be potentiated by cyclophosphamide treatment, by BCG administration, or by splenectomy. Treatments known to selectivity inhibit T-cell function suppressed the response.

Animals↗
PubMed1977

Use of glutaraldehyde-fixed goose erythrocytes in arbovirus serology.

Goose erythrocytes preserved with glutaraldehyde were compared with fresh cells in hemagglutination and hemagglutination-inhibition tests for arbovirus antigens and antibodies. The glutaraldehyde-fixed cells were as sensitive and specific as theresh erythrocytes and were stable at 4 degrees C for 6 months.

Animals↗
PubMed1977

The serological response to Yersinia pestis infection.

Passive haemagglutination antibody titres to Fraction I antigen of Yersinia pestis were plotted against day of clinical illness in 82 patients in Viet Nam. A rise was evident by day 5 with a peak at day 14, after which a plateau occurred. In contrast to all other patients, 2 patients with recurrent infections had elevated titres at the time of admission which decreased significantly during convalescence.

Aged↗
PubMed1975

Serologic observations during an outbreak of rat borne plague in the San Francisco Bay area of California.

Results of a serologic study of a plaque outbreak among rats (Rattus norvegicus) and associated wild rodents are presented. Bacteriologic and serologic evidence points toward mutual exchange of fleas and plaque infection between the intermingled rat and wild rodent populations. Results emphasize the value of serologic methods for epizootiologic studies of plague in North American rat populations.

Animals↗
PubMed1971

The effect of flea control on Yersinia (Pasteurella) pestis antibody rates in the California vole, Microtus californicus, and its epizootiological implications.

The effects of flea control on the prevalence of (Pasteurella) pestis antibody rates in the California vole, Microtus californicus, were studied in San Mateo County, California, USA. In this area, which had shown antibody prevalence rates of 50% or more during epizootics in previous years, continuous flea control measures reduced the antibody rate to virtually zero and held it at that level for 12 months, although a nearby area showed an incidence of 40-80% seropositive voles for 5 months during the same period. The striking correlation between the reduction of the flea population and the decline of Y. pestis antibody rates suggests that in the area studied there is no evidence that soil, or any factor other than the flea vector, was the agent of transmission of the infective agent in wild rodents.

Animals↗