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Biomedical subjects

B Watkins

Publications and source records attributed to B Watkins.

24 records · Page 2Linked to original sources
PubMed1986

Improved high-affinity monoclonal antibody to iododeoxyuridine.

Three monoclonal antibodies (Mabs), IU-1, IU-4, and B-44 were evaluated in enzyme immunoassays (ELISA) and by flow cytometry for their abilities to recognize bromodeoxyuridine (BrdUrd)- and iododeoxyuridine (IdUrd)-substituted DNA's, nucleotides, and nucleosides. IU-4 is a new Mab, derived from mice immunized with 5-iodo-2'-deoxyuridine-5'-monophosphate (IdUMP) conjugated (IdUMP) conjugated through the phosphate group to albumin. This immunogen was selected to resemble IdUMP in DNA. In competition ELISA assays, IU-4 prefers IdUrd to BrdUrd and prefers halogenated nucleotides over the corresponding nucleosides. In both ELISA and flow analysis, IU-4 recognizes IdUrd in DNA at substitution frequencies at least as low as one IdUrd one per 1,000 normal bases. The high affinity of IU-4 for IdUrd-DNA contrasts with IU-1 and B-44, which show a strong binding dependency on the frequency of base substitution and require DNA that is essentially fully substituted with BrdUrd for binding in both flow and ELISA assays. The high affinity of IU-4 for IdUrd in DNA and its independence of IdUrd residue spacing make it a superior reagent for the quantitative labeling of halogenated thymidine analogues in whole cells.

Animals↗
PubMed1980

The development of strategy use in a spatial task.

The ability of kindergarten, first grade, and third grade boys and girls to use a mnemonic strategy in a spatial task was investigated. Children (N = 90) were asked to replicate a model zoo. Use of an organizational strategy based on an object frame f reference was either required of the child, suggested, or not mentioned on the first trial. A second trial was conducted inwhich no strategy information was given. Analysis of the number of incorrect placements of model items indicated that the instruction had the greatest effect on the performance of the kindergarten children. Strategy instructions were also beneficial in reducing the time the children used to place items in the model but had no consistent effect on the amount of time the children used to examine the prototype.

Child↗
PubMed1977

Alpha wave biofeedback training therapy in alcoholics.

This investigation evaluated the therapeutic efficacy of alpha-wave biofeedback treatment for alcoholics. Twenty-five Ss were compared to a matched control group before and after administration of a 3-week alpha-wave biofeedback regimen on a wide variety of criteria that included State-Trait Anxiety, the MMPI, Multiple Affect Adjective Check List, Zuckerman's Sensation Seeking Scale, Watson's Anhedonia Scale, the Brief Psychiatric Rating Scale, and baseline alpha. The experimental Ss received 10 hour-long alpha training sessions. The experimentals showed more improvement than did controls on alpha production and two anxiety measure. Contradictory results appeared on two suspicion/paranoia measures. Alpha training appeared useful in the treatment of anxiety, but not other problems. However, the absence of significant correlations between amount of change on alpha and the anxiety measures suggests that the improvement may be due to a placebo effect.

Adult↗