Biomedical subjects

B Wiese

Publications and source records attributed to B Wiese.

42 records · Page 3Linked to original sources

PubMed1985

The distribution of 14C-chloramphenicol in the Japanese quail (Coturnix coturnix japonica).

The distribution of 14C-labelled chloramphenicol after oral and intravenous administration to egg laying Japanese quail was studied by whole-body autoradiography. In the liver, kidneys, gizzard, intestinal contents (bile) and oviduct, the 14C-concentration was higher than that of the blood short time after injection and remained higher than the blood up to 4 days. From 4 hrs, the concentration of 14C in the egg yolks was higher than that of the blood and from 24 hrs the radioactivity in the albumen of the eggs in the oviduct was also higher than that of the blood. The peak concentration in the egg yolk was found in the second egg laid 2-4 days after administration of 14C-chloramphenicol. In the albumen the maximum concentration was found in the first laid egg 24-48 hrs after administration. In the egg yolks, about 30% of the radioactivity represented unchanged chloramphenicol up to 5 days after administration. It was also shown that about 5% of the injected 14C-chloramphenicol was exhaled as 14CO2 during the first 12 hrs and about 37% of the dose was excreted in the combined faeces and urine during the same period of time.

Administration, Oral↗

PubMed1984

Plasma concentration, mammary excretion and side-effects of phenylbutazone after repeated oral administration in healthy cows.

Seven clinically healthy dairy cows were each given 2.5 g phenylbutazone (approximately 5 mg/kg body weight) by oral administration twice daily for 8 days. The concentrations of phenylbutazone in plasma and milk and several blood parameters were studied. The minimum plasma concentration during steady state was 100.4 +/- 7.3 micrograms/ml. During the same period the milk concentration never exceeded 1% of the plasma concentration. The elimination half-life in plasma was 38.6 +/- 3.7 h. Five days after administration had been discontinued, the milk concentration was 0.05 +/- 0.01 microgram/ml. All seven cows were clinically healthy throughout the experiment. The most pronounced side-effect of the blood parameters studied was a decreased concentration of leucocytes to about two-thirds of the control value. This might have a pronounced influence on the effectiveness of the immune system. There was also a significant decrease in total bilirubin indicating a decrease in the breakdown of erythrocytes.

Animals↗

PubMedDate not supplied

International nifedipine trial on anti-atherosclerotic therapy (INTACT)--methodologic implications and results of a coronary angiographic follow-up study using computer-assisted film analysis.

Animal experiments demonstrated a significant suppressive effect of various calcium channel blockers on the formation of atherosclerotic lesions. Therefore, a prospective, placebo-controlled, randomized, double blind multicenter study was performed to investigate the inhibitory influence of the calcium channel blocker nifedipine (80 mg/day) on the progression of coronary artery disease in man. Study endpoints were changes of coronary morphology documented by coronary angiography with particular respect to the formation of new coronary stenoses. In 348 out of 425 patients included in the study, coronary angiograms were repeated after three years. The angiograms were standardized by induction of a maximal coronary vasodilation with high doses of nitrates and by using absolutely identical angiographic projections. Quantitative analysis of coronary cineangiograms was performed with the computer-assisted contour detection system CAAS. Parameters were mean and minimal diameter of all segments and minimal stenosis diameter, percent diameter stenosis, length and plaque area of all stenoses. Continuous intake of study medication was registered in 282 patients, 134 on nifedipine and 148 patients on placebo. In these patients, a total of 3808 coronary segments with 893 stenoses (greater than or equal to 20% diameter reduction in at least one angiographic projection) were compared on the baseline and follow-up cineangiograms. The changes in all angiographic parameters analyzed averaged over all patients by considering all angiographic projections analyzed, indicated significant progression of the disease (p less than 0.006). The average changes in all parameters were even about three times more profound, when in the individual patients only the respective projections indicating the maximal changes were considered for the calculation (p less than 0.001). However, with neither of these two analysis modes, the differences in progression between the treatment groups were statistically significant. In the follow-up angiograms, a total of 196 new coronary lesions (185 stenoses, 11 occlusions) were found at previously normal arterial sites. In patients on nifedipine, an average of only 0.58 new lesions per patient were detected versus 0.80 lesions per patient on placebo (-27%; p = 0.031). INTACT is the first prospective angiographic trial on the progression of coronary artery disease using computer-assisted quantitative coronary angiography in such a high number of patients. All parameters analyzed indicated significant progression of coronary artery sclerosis. Nifedipine had no influence on the progression of preexisting coronary stenoses, but inhibited significantly the formation of new angiographically recognizable lesions. Further prospective coronary angiographic trials with calcium channel blockers using a comparably exact method are needed to confirm the results of this study.

Cineangiography↗

PubMedDate not supplied

Sex-dependency of hepatic alcohol metabolizing enzymes.

In mature female rats the administration of testosterone led to a striking reduction of hepatic alcohol dehydrogenase activity, whereas the hepatic microsomal ethanol oxidizing system as well as catalase were both increased in activity under these experimental conditions. Conversely, estradiol left the activities of all hepatic alcohol metabolizing enzymes virtually unchanged. Ovariectomy also had little if any influence on the activity levels of the enzymes. There was a clear difference between the sexes in the hepatic alcohol metabolizing enzymes with higher enzymic activities of the microsomal ethanol oxidizing system and catalase in male than in female rats, whereas the opposite constellation was found for alcohol dehydrogenase activity. These data therefore indicate the sex-dependent nature of alcohol dehydrogenase, the hepatic microsomal ethanol oxidizing system and catalase activities in rat liver.

Alcohol Dehydrogenase↗

PubMedDate not supplied

[Nutritional iodine status of healthy infants in Germany].

In 78 healthy term babies from Düsseldorf and surroundings spontaneous urine samples were collected on day 108 to day 144 of life and analyzed for the content of iodine and creatinine. 26 babies were breast fed. 23 babies received an iodized hypoallergenic formula and 26 babies different commercial formulas only partly iodized. From 4 parameters to estimate iodine supply of the babies (urinary iodine concentration microgram/dl, iodine/creatinine ratio microgram/g; estimated daily iodine excretion microgram/d, estimated daily iodine excretion corrected for body surface area microgram/dl 1.73 m2) estimated daily iodine excretion was the most useful parameter. Breast fed babies showed a significantly lower estimated daily iodine excretion (median 32 microgram/d) than babies fed the hypoallergenic (52 microgram/d) or a commercial formula (54 micrograms/d). In Germany with an insufficient iodine supply of pregnant and lactating mothers breast fed infants run a risk for an insufficient iodine supply. Furthermore, supplementation of infant formulas with iodine started in 1990/1991 proved to be a sufficient measure to correct iodine deficiency.

Breast Feeding↗

PubMedDate not supplied

Comorbid anxiety and affective disorder in alcohol-dependent patients seeking treatment: the first Multicentre Study in Germany.

The goals of this study were to describe demographic variables, drinking history, and the 6-month prevalence of Axis I comorbidity among alcohol-dependent subjects in GERMANY: The variables: amount of alcohol consumption, age at onset of the first alcohol consumed, age at onset of daily alcohol consumption, age at onset of withdrawal symptoms and number of detoxifications were related to the different comorbid disorders and gender. In this study, 556 patients from 25 alcohol treatment centres were enrolled between 1 January 1999 and 30 April 1999. After a minimum of 10 days of sobriety patients who fulfilled ICD-10 and DSM-IV criteria of alcohol dependence were interviewed for data collection using the Mini-DIPS (German version of the Anxiety Disorders Interview Schedule) and a standardized psychosocial interview. The 6-month prevalence of comorbid Axis I disorders was 53.1%. Among the patients with comorbidity, affective and anxiety disorders were most frequent. Comorbid stress disorder was associated with an early start of drinking, an early beginning of withdrawal symptoms, highest number of detoxifications, and the highest amount of alcohol consumed. Female patients with anxiety disorder consumed more alcohol and started earlier than females without this comorbid disorder. The data do not answer the question of the pathogenesis of comorbid disorders and alcoholism, but indicate that stress disorders in alcoholic patients and anxiety disorders in female alcoholics influence the course and severity of alcoholism.

Adult↗