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Biomedical subjects

B Wille

Publications and source records attributed to B Wille.

At least 19 recordsLinked to original sources
PubMed1999

[Effect of sotalol on systemic hemodynamics and electrophysiology in patients with life-threatening ventricular tachyarrhythmias].

OBJECTIVE: To evaluate the systemic hemodynamics and electrophysiology effect of intravenous and oral sotalol and its tolerance of long-term oral therapy in patients with life-threatening ventricular tachyarrhythmias. METHODS: Twenty-three patients, 17 with sustained ventricular tachycardias (VTs), 6 with ventricular fibrillation (VF) were studied. 16 patients were postmyocardial infarction, 4 were right ventricular dysplasia and 3 were dilated cardiomyopathy. RESULTS: Sotalol prevented the induction of arrhythmias in 34.3% with bolus intravenous injection (1 mg/kg body weight) and 60.8% with oral (160 mg twice daily for 10 to 14 days). Both intravenous and oral sotalol led to bradycardia, decreased in cardiac index, increased in left ventricular filling pressure as well as in systemic vascular resistance. The stroke volume increased significantly after sotalol intravenous, but not changed at peak action after oral sotalol. During a follow-up of 3 to 6 months, oral sotalol prevented the recurrent of arrhythmias in 12 of 16 patients who were response to electrophysiology testing. Congestive heart failure associated with a marked bradycardia developed in one patient. This patient was managed with a reduction in the dosage and with a regimen of digoxin and furosemide. CONCLUSION: Sotalol provided effective prophylaxis against life- threatening Ventricular tachyarrhythmias. It exerted significant negative hemodynamics effects but with quite good clinical tolerance, even in patients with compromised heart function.

Administration, Oral↗
PubMed1993

Interaction of immunologically-active lipopeptides with membranes.

Synthetic tripalmitoyl-S-glycerylcysteinyl (Pam3Cys) peptides are derived from the N-terminal part of bacterial lipoprotein and constitute polyclonal B-lymphocyte and macrophage activators. In order to elucidate the primary events of leukocyte activation, we investigated the biophysical interaction of lipopeptides containing spin labels or fluorescent markers with phosphatidylcholine vesicles or immune cells. Utilizing fluorescence microscopy and FACS analysis we found, that the surface of cells, after incubation with a fluorescein-labelled lipopeptide, was highly fluorescent. In addition, capping and patching was observed. Furthermore, fluorescence quenching experiments and electron paramagnetic resonance studies using vesicles incubated with lipopeptides suggested, that the peptide moiety and other more polar molecules linked to the lipo-amino acid are exposed to the hydrophilic compartment. These results show that in lipopeptide conjugates the Pam3Cys moiety acts as an efficient membrane anchor for molecules covalently coupled to it. The sequestering of the fatty-acid chains of the lipopeptide within the membrane is an early step of interaction, which might induce the uptake of the lipopeptide into the cell and the stimulation of immunocompetent cells.

Amino Acid Sequence↗
PubMed1992

[Possibilities for preventing the epidemic spread of rotavirus infections on neonatal wards].

Rota viruses are the most frequent cause of acute gastroenteritis of infants and toddlers. Small epidemies occur enlarged in institutions for newborns and toddlers. The infection occurs normally via faeces. An aerogenes infection is in discussion. In case of a suspected Rota-Virus-Infection in an infant- or toddler care unit the patients have to be isolated immediately or quarantined. Further essential precautions preventing the spreading are mentioned. With this regime we could only see individual Rota-Virus-Infections in infant care units.

Child, Preschool↗
PubMed1991

[Doppler ultrasound study in patients with post-thrombotic syndrome: a screening method].

Doppler ultrasound has been a useful, noninvasive technique in the diagnosis of deep venous thrombosis. Until now, primary or secondary varicosities could only be clarified through phlebography or strain-gauge plethysmography. The aim of our study was to assess the flow time in 28 patients with primary varicose veins and postthrombotic syndrome using Doppler ultrasound. The Wilcoxon-Test was used for statistical reviewing in the second test, revealing a significant difference between both groups. The confidence intervals did not overlap.

Adult↗
PubMed1989

Location and dynamics of alamethicin in unilamellar vesicles and thylakoids as model systems. A spin label study.

Location and dynamics of the voltage-dependent pore-forming icosapeptide alamethicin have been studied using spin labels which were linked directly and via spacers to the C-terminus of the amphiphilic alpha-helix. Ion-transport activities of these derivatives were found to be very similar to those of natural alamethicin in green plant thylakoids chosen as a model system. The shape of the electron spin resonance spectra indicates segmental motion of the nitroxide rather than rotation of the whole peptide. A population of spins showing narrow lines in the presence of thylakoids or lipid vesicles is attributed to alamethicin in the aqueous solution. A second population shows rotational correlation times greater than 10(-9) s and is bound to the membranes, the C-termini residing in an environment with a polarity close to that of water. This population is inaccessible to the hydrophilic, charged line broadening agent chromium oxalate. Since spectral shapes and amplitudes of spectra are unchanged by additions of unlabelled peptide, it is concluded that the ESR detectable spins are bound to peptides essentially in the monomeric state. Alamethicin induced pore formation under flash illumination is demonstrated by measurement of kinetics of proton deposition in the thylakoid interior. When pores are opened by illuminating thylakoids and thus applying a membrane potential, mainly the bound population is affected by a process reversibly suppressing the signal, whereas only limited disappearance of label from the external medium is detected. Apparently, the potential causes a change in the conformation of the peptide which leads to a further immobilisation of the label, possibly due to a deeper insertion of the alpha-helices into the lipid membrane. However, evidence has been presented experimentally that there is no detectable change of potential prior to the opening of the pore.

Alamethicin↗
PubMed1989

[Simulated tachyarrhythmia. A case report].

In this case report, the hospitalization of a 34-year-old woman with a history of heart disease is described. Arrhythmias and precordial chest pain led to her admission to the coronary care unit. Different therapeutic trials failed. Finally it became obvious that all tachycardias documented by the arrhythmia monitoring system were artifacts, produced by manipulation at the ECG electrodes. After disclosure and confrontation, a psychic misdevelopment in combination with an abuse of analgetics was found.

Adult↗
PubMed1976

[Possibility of the development of resistance to disinfectants in microorganisms (author's transl)].

Germs resistant to disinfectants have been found by various authors in hospitals. The question arise whether this is a case of primary resistance or - similar to resistance to antibiotics - a development of resistance takes place. Five fully sensitive laboratory germs were exposed for longer periods of time to sublethal doses of four basic constituents of disinfectants (phenole, isopropanole, formaldehyde, chloramine 80). After a different number of passages an increasing resistance could be noted against formaldehyde and chloramine 80. To date no comments are possible regarding the mechanisms of action.

1-Propanol↗
PubMed1976

[Results of hygienic investigations in hospitals and their consequences (author's transl)].

In all parts of the hospitals examined results were found, which demonstrated, in part considerable, gaps in the hygienic systems. Therefore the enforcement of the following measures must be emphatically postulated: - Development of hospital hygiene plans by competent experts in cooperation with the doctors and nurses in charge. - Foundation of commissions for hygiene, who are responsible for preventive measures and for the establishment of a survey of hospital infections. - Delegation of the internal control of hospital hygiene to a person experienced in that field without other functions. - Renovation of supplementation of the technical hygienical facilities especially in old hospitals.

Cross Infection↗