Biomedical subjects

B Wyhowski de Bukanski

Publications and source records attributed to B Wyhowski de Bukanski.

6 recordsLinked to original sources

PubMed1992

Immunoaffinity-chromatography purification of salbutamol in liver and HPLC-fluorometric detection at trace residue level.

A method combining immunoaffinity-chromatography (IAC) and high-performance liquid chromatography (HPLC) for the analysis of Salbutamol in liver with a low quantification limit of 1 micrograms/kg has been developed. Salbutamol was extracted with 0.01 mol/L HCl and purified by IAC. The samples were analysed on a liquid chromatograph fitted with a C18 mu-Bondapak column. A fluorometer was used for the detection of salbutamol. Recoveries of 67-80% could be obtained.

Albuterol↗

PubMed1991

Determination of levamisole and thiabendazole in meat by HPLC and photodiode array detection.

An HPLC method for the analysis of levamisole and thiabendazole has been developed with recoveries varying over 63-75%. The two anthelmintics are extracted from meat with ethyl acetate, purified by liquid/liquid extraction and analyzed quantitatively on a mu Bondapak C18 column. The optimum detection is achieved by means of a photodiode array detector at 240 nm for levamisole and at 300 nm for thiabendazole. The detection limits for both compounds in meat are 25 micrograms/kg and 5 micrograms/kg, respectively.

Animals↗

PubMed1991

Cimaterol and clenbuterol residue analysis by HPLC-HPTLC in liver.

A method has been developed for the analysis of cimaterol and clenbuterol residues in liver, with detection limits of 0.25 micrograms/kg and 0.5 micrograms/kg, respectively. The recovery varied from 55% to 60%. After extraction, a clean-up procedure with Baker-spe C-18 columns was performed. The two chemical compounds of interest were eluted with methanol. Cimaterol and clenbuterol were quantitatively determined by high-performance liquid chromatography (HPLC) using an RP-Select B (5 microns) column and a post-column reaction procedure. The positive results were confirmed by high-performance thin-layer chromatography (HPTLC) as this technique reaches the same level of sensitivity as the HPLC method.

Adrenergic beta-Agonists↗

PubMed1991

Salbutamol identification in liver and urine by high-performance thin-layer chromatography and densitometry.

This paper describes a rapid method for the identification of salbutamol in liver and urine. Salbutamol is extracted from liver with an acid solution, purified on Baker columns and eluted with methanol. After derivatization, salbutamol is detected on HPTLC plates as a blue spot. Urine samples are directly purified on the C18 columns and then the same procedure is followed as for the liver samples. Using this screening method, salbutamol can be semi-quantitatively determined at the micrograms/kg level.

Albuterol↗

PubMed1991

Determination of thiabendazole residues in meat by HPLC using ultraviolet and fluorometric detection.

An HPLC method is described for the residue analysis of thiabendazole in meat. The recovery varies from 62 to 75%. Thiabendazole is extracted from the tissue using 3 mol HCl, eluted from the Extrelut-20 column with dichloromethane and then injected onto a C18 column. The optimum conditions for detection are described using ultraviolet and fluorescence spectroscopy. The sensitivity is such that thiabendazole can be determined at a level of 5 micrograms/kg meat. The absolute detection limit with fluorometry is 100 pg.

Animals↗

PubMed1988

A two-dimensional high-performance thin-layer chromatographic screening method for sulphonamides in animal tissues.

A procedure for the identification of sulphonamides in edible animal tissues by two-dimensional high-performance thin-layer chromatography is described. Sixteen sulphonamides can be detected at an absolute level of 10 ng. The absolute detection limit of the sulphonamide standards is 1 ng. After extraction of the sulphonamides with chloroform/acetone, the acidified extract is concentrated and purified using a cation-exchange solid phase extraction column. The column is treated with ammonia vapour and the sulphonamides are then eluted with methanol. Blank samples of edible animal tissues spiked with sulphonamides (frequently used in Belgium) result in very good separation.

Animals↗