PubMed Health⌕ Search

Biomedical subjects

B Y Liu

Publications and source records attributed to B Y Liu.

18 recordsLinked to original sources
PubMed1991

Experimental infection of specific-pathogen-free mice with enterohemorrhagic Escherichia coli O157:H7.

By subcutaneous inoculation of 10(8) CFU of enterohemorrhagic E. coli O157:H7, specific-pathogen-free mice revealed most of the symptoms and histological changes observed in patients. The histological changes in intestine were mainly seen in the distal parts of small intestine and the cecum. Vacuolation of villi in the cecum was also observed. The histological changes in the kidneys of the infected mice were featured as the swollen epithelial cells of glomeruli and the marked thickening of glomerular capillaries with barely visible lumens. Unexpected findings in the bronchiole were characterized by sloughing of the epithelial cells of bronchiolar wall, leading to partial or complete obstruction of the lumens. Histological changes in the spleen, liver and lymphnodes were also observed. The bacteria were recovered from the feces, contents of small intestine, and samples taken from kidney, liver, heart, spleen, different parts of small intestine, cecum, and colon. By using peroxidase-antiperoxidase (PAP) assay with polyclonal antibodies against "O" antigen of E. coli O157:H7, it was observed that the samples taken from the brain, kidney, ileum, cecum, spleen, and liver gave positive reactions. Feces and contents of small intestine obtained from all of the infected animals were positive by occult blood test. These results show that the experimental infection of E. coli O157:H7 in this model is systemic in nature.

Animals↗
PubMed1991

Detection of human papilloma virus (HPV) in laryngeal carcinoma tissue.

Using alpha-32P-dCTP labelled human papillomavirus type 16 DNA genome probe (cHPV 16 DNA, cloned and identified from a Chinese cervical invasive carcinoma), 3 HPV 16 genomic relative sequences were detected in 6 biopsies of laryngeal carcinoma. The results showed serious HPV infection in laryngeal carcinoma. This indicates that HPV infection may play an important pathogenetic role in cancer development.

Blotting, Southern↗
PubMed1991

[Hypoxaemia during transportation of patients after anesthesia and upper abdomen surgery].

The incidence of hypoxaemia was studied in 100 patients breathing room air during their transportation from the operating room to the recovery room. Hypoxaemia (SaO2 less than 90%) occurred in 24 (24%) patients. Oxygen saturation rose quickly above 95% when oxygen was administered. The causes of hypoxaemia are discussed. Quick transportation and oxygen supply were recommended.

Abdomen↗
PubMed1990

[Effects of extracellular sodium concentration on the sodium-pump activity in Purkinje fibres of sheep].

Intracellular sodium activity (aiNa), extracellular potassium activity (aok) and resting membrane potential (Vm) were measured in sheep cardiac Purkinje fibres exposured intermittently to calcium-free solution containing different concentrations of low sodium. In Ca-free low sodium solution, aiNa decreased, the rates and amplitudes of aiNa changes were related to [Na]o linearly. The linear correlation between the stable values of aiNa (aiNa(S)) at 6th minute of aiNa decrease and [Na]o meant that the amplified active Na+ extrusion and pump current resulting from decreased [Na]o, kept finally Na+ gradient across the membrane relatively stable. Hence, it was proposed that it was the Na+ gradient across the membrane but not the internal Na+ that regulated the sodium pump activity. The membrane hyperpolarization which was incident with the increase of active Na+ extrusion was caused mainly by the amplified pump current and embellished by the intercellular K+ depletion and accumulation simultaneously, which was suggested by the mutual relation of time courses of changes in aiNa, Vm, aok during the exposure of the fibre to 1.3Na Ca-free solution.

Animals↗
PubMed1987

[Preliminary study on the nucleic acid hybridization technic in detecting HSV-2 DNA corresponding sequence of cancer tissues of the uterine cervix].

In this paper, 34 samples, 25 from 18 cervical carcinoma patients and 9 from 8 non-cervical carcinoma patients, were assayed using (alpha-32P) dCTP HSV-2 DNA probe and nucleic acid hybridization technic. It was shown that the positive rates of HSV-2 DNA corresponding sequence were 50% (2/4), 36% (5/14) and 12% (1/8) in the in situ cancer, invasive cancer and non-cervical cancer tissues. No HSV was isolated from 7 cancer specimens which showed a positive reaction by nucleic acid hybridization.

Animals↗
PubMed1987

Preparation of large temporomandibular joint specimens for pathological study.

Ten fresh temporomandibular joint (TMJ) specimens about 5 X 4.5 X 3.5 cm in size were removed at autopsy by 5 cuts according to appropriate anatomical landmarks. After routine formalin fixation, the whole-TMJ specimens were wrapped with a thin layer of self-curing resin and then cut with a low speed bone saw along the parasagittal plane predetermined by x-ray guidance. Each specimen was serially cut into 4 to 5 parallel slices of 3 mm thickness, which were then decalcified with 14% EDTA and embedded in paraffin. Histological sections of 5 microns were stained with hematoxylin and eosin. The procedures were accomplished within 20 to 24 days after autopsy. With this technique, the anatomical interrelationships among the various joint components could be maintained and the macroscopic and microscopic topography of the TMJ could be studied in the desired reference plane. Therefore, the corresponding changes among the joint components in a diseased TMJ could be thoroughly examined. This technique was also applicable for the study of large specimens containing both hard and soft tissues.

Adolescent↗
PubMed1978

A dust generator for laboratory use.

A dust generator has been developed to produce dry dust from powderous materials for instrument calibration and laboratory studies. The powder is fed at a constant rate via a chain conveyor into a fluidized bed where the particles are deagglomerated and aerosolized. The powder feed rate is raviable from 1.2 to 36 mm3/min and the air flow rate from 9 to 30 Lpm. Aerosols of coal, silica, potash, rock, and Arizona road dust have been successfully generated for calibrating optical particle counters and aerosol mass monitors.

Aerosols↗
PubMed1975

An aerosol generator of high stability.

This paper reports on the use of a syringe pump to provide a constant liquid flow to an atomizer to obtain an aerosol generator of high stability. This syringe-pump atomizer has also been used with a vaporizer-condenser to generate monodisperse DOP (di-octyl phthalate) aerosols in the 0.032 to 1 mum diameter range.

Aerosols↗
PubMedDate not supplied

Three wishes of a child with cancer.

Thirty-seven children in Taiwan with cancer, ranging in age from 4 to 16 were asked to identify their three wishes. Nearly three-quarters of the total sample "wished" to go home from the hospital and 90% used one of their wishes in hopes to be cured. Preschool youngsters were more eager to resume play activities (64%), to go home (36%), and to own some desired object (36%); while school-age children hoped primarily to be cured (44%), to go home (39%) and to have more physical exercise (28%). Adolescents wished to have their disease cured (100%), return to school (63%) and to go home (37%). Gender differences seemed to have little impact on the wishes of children. The following study underscores the need for nurses to understand the hopes and dreams of children with cancer in order to work with these children toward their own envisioned futures while making the present as least traumatic as possible. Indeed, such mutual goal-setting is at the heart of the therapeutic nursing process for clients of any age and any culture.

Adolescent↗