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Biomedical subjects

B Yu

Publications and source records attributed to B Yu.

At least 217 records · Page 12Linked to original sources
PubMed1991

[Quality of tuberous root of Liriope spicata (Thunb.) Lour. var. prolifera Y.T.Ma and Ophiopogon japonicus (L.F.) Ker-Gawl.--comparison of immune function].

This paper deals with comparative study on the immune function of the tuberous root of Liriope spicata var. prolifera and Ophiopogon japonicus. The results showed that the aqueous extract of both species mentioned above could increase obviously the spleen weight (immunity organ) of mice, enhance the clearance rate of iv charcoal particles in mice and antagonize remarkably the leukopenia caused by cyclophosphamide.

Animals↗
PubMed1991

A clinical and laboratory study of ciclopirox olamine (8% Batrafen) in the treatment of onychomycosis.

Ciclopirox olamine, a new synthetic substituted pyridone derivative, is an antimycotic agent with activity against a broad spectrum of pathogenic and nonpathogenic fungi. The clinical efficacy and safety of 8% ciclopirox olamine nail liquor were evaluated in 100 cases with finger (or great toe) onychomycosis. After 16 weeks and 24 weeks of treatment for finger and great toe onychomycosis, respectively, the overall therapeutic results were excellent in 36 cases, good in 17, fair in 24, and poor in 23. The period of treatment was extended in 31 cases; among them, 10 cases showed further improvement. As for in vitro inhibitory activity, the MIC of ciclopirox olamine against T. rubrum and C. albicans was 1 to 4 mg/L and 1 to 16 mg/L, respectively. This study indicates that 8% ciclopirox olamine nail liquor, with its satisfactory efficacy and lack of side effects, is a good remedy for onychomycosis.

Adult↗
PubMed1991

The role of diacylglycerol/protein kinase C signaling in insulin-stimulated glucose transport.

There seems to be little doubt that insulin rapidly perturbs phospholipid metabolism, and this appears to increase DAG/PKC signaling in many target tissues. Considerable new evidence further suggests that DAG/PKC signaling plays an important role in insulin-stimulated glucose transport. Further studies are needed to test this hypothesis and examine its importance in states of clinical insulin resistance.

Animals↗
PubMed1991

[Research on the relationship between central acetylcholine and acupuncture analgesia].

The article systematically reported our physiological and biochemical work on the relationship between central acetylcholine and acupuncture analgesia. The results indicate: 1. Acupuncture on acupoints could produce analgesia. 2. Ach contents in cerebrospinal fluids and brain increased under acupuncture analgesia. 3. AChE activities iu brain elevated under acupuncture analgesia. 4. ChE inhibitor reinforced the effects of acupuncture and inhibitor of ACh synthesis could inhibit the effects of acupuncture, which could be reversed by administration of ACh and chlorocholine. M-AChR antagonists could also inhibit the effects of acupuncture. 5. The turnover rate of ACh in diencephalon, caudate nucleus and spinal dorsal horn accelerated when acupuncture analgesia. The discussion was given on the variation of metabolic dynamics in combination with related literature.

Acetylcholine↗
PubMed1990

Expression of growth-related genes during tumor progression in v-raf-transformed rat liver epithelial cells.

Clonal cell lines were derived from rat liver epithelial cells following their transformation with either v-raf or v-raf/v-myc. Cells transformed with v-raf alone showed reduced tumor incidence and tumor growth rates when implanted into nude mice, compared to cells also expressing the v-myc oncogene. A series of additional clones isolated from a tumor obtained following inoculation of an athymic nude mouse with the v-raf-transformed rat liver epithelial cells displayed an intermediate range of tumor aggressiveness. These findings indicate that unknown genotypic and/or phenotypic changes occur during tumor formation in vivo, which are required in addition to raf activation for complete expression of the malignant phenotype. This in vitro model of tumor progression was used to examine alterations in the expression of genes related to the growth control of liver epithelial cells, which may be involved in the malignant conversion of the preneoplastic cells. A close association was observed between the increased level of expression of the transforming growth factors alpha and beta 1, the decreased expression of extracellular matrix proteins fibronectin and collagen I, and the tumor aggressiveness (latency/growth rate), suggesting a causal role for these factors in the progression of v-raf-transformed rat liver epithelial cells to the fully malignant phenotype.

Animals↗
PubMed1990

Miniaturization of a liquid membrane sensor for the determination of bile acids.

The preparation of a compound liquid ion exchanger used for the miniaturization of bile acid sensitive microelectrode is described. The liquid ion exchanger is 2% benzyldimethylhexadecylammonium cholate in decanol/0.1 M hexadecyltributylammonium taurocholate in 5% hexachlorobenzene, 0.5% bromoacetanilide O-dichlorobenzene. The slopes, detection limits, selectivity coefficients, drifts and response time of the various bile acid sensitive microelectrodes are evaluated and compared.

Bile Acids and Salts↗
PubMed1990

Detection of Candida antigenuria in disseminated candidiasis by immunoblotting.

Immunoblotting (Western blotting) was used to detect Candida albicans antigens in urine of 10 patients with disseminated candidiasis who had two or more positive blood cultures. Twelve urine samples were examined; and antigenuria was found in five of six patients with C. albicans infections, in one patient with a mixed Candida infection (including C. albicans), and in one of two patients with C. tropicalis infection. All except one specimen was collected from 2 to 12 days after initiation of amphotericin B therapy. Positive samples showed different numbers of bands in Western blots with an antigen that had an apparent molecular weight of 47,000 in common. This antigen was not found in the urine of patients who had more than 5 days of therapy for candidiasis and who were responding to therapy. The results suggest that Western blotting for C. albicans antigens in urine may be a useful method for the diagnosis of disseminated candidiasis and for evaluating antifungal treatment.

Amphotericin B↗
PubMed1990

Use of immunoblotting to detect Aspergillus fumigatus antigen in sera and urines of rats with experimental invasive aspergillosis.

Immunoblotting was used to detect Aspergillus fumigatus antigen in sera and urines of immunosuppressed rats experimentally infected with A. fumigatus. Organisms were administered by both intravenous and intratracheal injections. Intravenously infected rats developed disseminated aspergillosis, but intratracheally infected rats developed pulmonary disease only. Fungal cultures of blood and urine samples from infected rats were negative. In the urines of intravenously infected rats, antigen was detected 24 to 48 h after infection; in the urines of intratracheally infected animals, antigen was detected on days 4 to 5 after infection. Antigen in serum was detected later than antigen in urine was. Following sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting of serum and urine samples, the most strongly reacting antigenic materials were found in the 88-, 40-, 27-, and 20-kilodalton regions. These dominant antigens appeared to be the same as those of control antigens prepared from A. fumigatus grown in vitro. Rabbit antiserum to Aspergillus filtrate antigen was found to be more immunoreactive than antiserum to mycelial or conidial antigen. No mycelium-specific antigens were detected.

Animals↗
PubMed1990

[Assay of bilirubin of ox gallstone in Chinese patent medicines by differential spectrophotometry].

This paper describes that the absorption spectrum of bilirubin solution was changed obviously by photo-oxidation. The absorbance was measured at 453 nm before and after light irradiation. The contents of bilirubin of ox gallstone in Liuying pills, Liushen pills and Niuhuang Xiaoyan pills were determined by differential absorbance (delta A). Calibration graph was linear in the range of 1.6-8.0 micrograms/ml for delta A. The average recoveries for three Chinese patent medicines were over 93%. Determinations of bilirubin in Chinese patent medicines were not affected by other components.

Animals↗
PubMed1990

[Clinical analysis of 6 cases of pyoderma gangrenosum].

This article reports 6 cases of pyoderma gangrenosum of either acute or chronic clinical type. Etiologically, we found that minor local trauma could induce ulcerative and destructive lesions, with typical pyoderma gangrenosum features following in 4 cases. All the patients responded well to systemic corticosteroids. In comparison with cases reported in other countries, no systemic diseases (including ulcerative colitis, Crohn's disease and polyarthritis) were found in our series. Cultures taken from early pustular lesions were sterile. Histopathological examination showed heavy neutrophilic infiltration in the dermis, and no evidence of vasculitis was found in the biopsies. Immunological investigations revealed no specific reactions in our cases.

Adolescent↗
PubMed1989

Direct N-terminal sequence analysis of rat liver plasma membrane glycoproteins separated by two-dimensional polyacrylamide gel electrophoresis.

Nine previously uncharacterized membrane glycoproteins from normal rat liver have been analyzed by amino acid sequencing from two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) after transblotting to Immobilon-P membranes. Three of these components show altered levels of expression in liver tumors. A single electroblotted polyacrylamide gel yielded sufficient quantities of these glycoproteins for amino acid sequencing and the N-terminal structure could be determined for four of them. The remaining five glycoproteins of interest were not sequenceable in this manner, presumably because they had blocked N-termini. Prior to electrophoresis, two enrichment methods were applied to the crude liver membrane preparations: affinity chromatography with concanavalin A to isolate the plasma membrane glycoproteins and then fast protein liquid chromatography on Superose 12 to obtain components having a specific range of molecular weights. These materials were next subjected to 2-D PAGE using pH 4-6 carrier ampholytes in the first dimension and 7.5% sodium dodecyl sulfate gels in the second. The proteins were then electroblotted to Immobilon-P membranes and located by staining with Coomassie Brilliant Blue R-250. Our results demonstrate that N-terminal sequencing (gas-phase) can be achieved on polypeptides obtained from approximately 250 micrograms of total glycoproteins applied to a single 2-D gel.

Amino Acid Sequence↗
PubMed1989

A new macromolecular antitumor antibiotic, C-1027. III. Antitumor activity.

C-1027, a new macromolecular antitumor antibiotic produced by Streptomyces globisporus C-1027, showed extremely potent cytotoxicity toward cultured cancer cells. Compared in terms of IC50 values, antibiotic C-1027 showed much more potent cytotoxicity than doxorubicin, mitomycin C and neocarzinostatin. Spermatogonial assay, a prescreen for anticancer drugs, was highly sensitive for detection of C-1027. At tolerable doses, C-1027 exhibited marked inhibition on a panel of transplantable tumors in mice, which included leukemia L1210, P388, ascites hepatoma H22, sarcoma 180 and melanoma Harding-Passey.

Aminoglycosides↗
PubMed1989

[Specific binding and internalization of anti-CCT2 monoclonal antibody and bleomycin A6 conjugate in human leukemia cells].

The immunoconjugate of anti-CCT2 monoclonal antibody linked to bleomycin A6 was adsorbed on colloidal gold particles (McAb-A6-Au). Binding and internalization of McAb-A6-Au particles in human leukemia CEM cells were examined by electron microscopy. After 60 min at 4 degrees C, McAb-A6-Au particles were bound to the surface membrane of 78% of CEM cells. Transferring to 37 degrees C for 15 min, McAb-A6-Au particles were found to be 56% inside the CEM cells and about one third of the cells contained particles in the nucleus. After 4 h at 37 degrees C the percentage of CEM cells containing McAb-A6-Au particles increased to 72%. However, only 14% of the antigenically irrelevant U937 cells contained these particles and none of them was found in the nucleus. Preincubation with unconjugated anti-CCT2 monoclonal antibody markedly blocked the McAb-A6-Au particle uptake in CEM cells. The McAb-A6-Au particles were internalized through the formation of endocytotic vesicles. In addition, some McAb-A6-Au particles were able to penetrate the plasma membrane directly into cytoplasma and notably into the nucleus. Results indicate that the immunoconjugate of monoclonal antibody linked to bleomycin A6 showed selective binding to target cells and entered the cells specifically and rapidly.

Antibodies, Monoclonal↗
PubMed1989

[Preliminary study of atrial natriuretic peptide in patients with cardiac deficiency and its clinical significance].

Immunoreactive atrial natriuretic peptide (irANP) has been measured in 24 patients of various cardiac diseases by radioimmunoassay. According to the TCM, these patients were divided into 4 groups: cardiac energy deficiency mild (A) and severe (B) groups; simple cardiac deficiency (C); and the control (D). The New York Heart Association (NYHA) classification of cardiac function in 5 cases of group A were all degree IV; in 6 cases of group B were 2 of degree IV and 4 of degree III; in 6 cases of group C were 1 of degree III, 3 of degree II and 2 of degree I; and in 6 cases of group D were all degree I. The results of chest X-ray and echocardiogram suggested that the classification of cardiac function was objective. The quantity of irANP in group A-D and NYHA IV-I decreased gradually and was correlated with left ventricular ejection fraction (r = -0.87, P less than 0.01). The average amounts of irANP in the different TCM groups A-D were at the equivalent levels in the groups NYHA IV-I. Meanwhile it was found that the cardiac energy deficiency patients had abnormality in some parameters of hemorrheology, but no correlation with irANP. It suggested that the diagnosis of cardiac deficiency by TCM was correlated with the different degree of NYHA in the sense of biochemical index of irANP. The irANP might be considered as one of the objective signs of the cardiac energy deficiency, which also might represent the severity of the disease.

Atrial Natriuretic Factor↗
PubMed1989

[Experimental study on the antigoiter effect of high casein nutrition].

In this experiment, goiter was successfully induced in rat with MMI, and the antigoiter effect of 25% and 50% casein diet was observed. The results showed that the diet with 50% casein is more effective than that with 25% casein in counteracting MMI and preventing goiter in rat. The antigoiter mechanisms of high protein nutrition might be as follows: Protecting the mechanism of thyroid iodine transportation through the follicular cell, and therefore accelerating thyroid iodine metabolism; Relieving thyroid peroxidase (TPO) from the inhibitive effect of MMI and facilitating thyroid hormone synthesis; Coordinating the function of hypothalamus-pituitary-TSH-thyroid axis and protecting the wholeness of thyroid cell and thus avoiding the occurrence of pathological changes.

Animals↗
PubMed1988

Differential attitudes of Chinese students toward people with disabilities: a cross-cultural perspective.

The purpose of this study was to examine the attitudes of 338 Chinese secondary school students toward three major disability groups (people who are physically disabled, emotionally disturbed, and mentally retarded) using an adaptation of the American Scale of Attitudes toward Disabled Persons (SADP). We found that physically disabled persons were rated higher across the three subscales of the SADP compared to either emotionally disturbed and mentally retarded individuals, who were rated similarly low by the students. The lack of differentiation between the two mental handicapping conditions is at variance with contemporary western findings. The overall negative attitudes toward people with mental disabilities may have significant implications for community rehabilitation programming for this population.

Adolescent↗