Biomedical subjects

B Z Li

Publications and source records attributed to B Z Li.

14 recordsLinked to original sources

PubMed2005

Establishment of stable HeLa cell lines expressing enzymatically active hepatitis C virus RNA polymerase.

The hepatitis C virus RNA polymerase (NS5B) is strictly required for viral replication and thus represents an attractive target for antiviral drug development. In this study, stable HeLa cell lines with an integrated NS5B gene were selected by G418 and then confirmed by genome PCR. Subsequently, transcription and expression of the integrated NS5B genes were demonstrated by RT-PCR and Western blot analysis. Further analysis demonstrated enzymatic activity of the expressed NS5B polymerase. The stable HeLa cell lines should be useful for the identification of NS5B inhibitors and for studying the mechanisms of HCV replication.

Gene Expression↗

PubMed2001

[The measurement of IAA oxidase in "ping guo li" leaves].

IAA oxidase activity during the fruit bud differentiation of "Ping Guo Li" in leaves measured by UV-Vis method was studied. The method is less sample, simple operation and high sensitivity. The test of recovery and repetition shows that both the precision and accuracy accord with the demands.

Malus↗

PubMed2001

[Development and application of the facial soft tissue 3D measuring system].

OBJECTIVE: To combine measurement and image technology as an objective means to help prosthodontists provide better service. METHODS: The system was developed by consisting of two CCD cameras, real time hardware for the recognition of markers and software for the 3D reconstruction. RESULTS: It allowed efficient, direct and noninvasive calculation of the 3D liner and angular measurements of the human face. CONCLUSION: This system is a useful way to get 3D information of the human face. It will be a good supplement to the classic cephalometric analyses.

English Abstract↗

PubMed1994

[Clinico-pathologic study of 285 cases of ectopic pregnancy in intrauterine devices-users and non-intrauterine devices-users].

Two hundred eighty five cases of ectopic pregnancy in Beijing area from Jan. 1 to Dec. 31 1990 including 150 cases of intrauterine devices (IUD) and 135 cases of non-IUD users were studied. Clinico-pathological observation included the history of pelvic inflammatory disease, the site of ectopic pregnancy, the classification of previous tubal inflammation of the ectopic side and other pathologic lesions, the side of the corpus luteum of pregnancy and histology of endometrium, etc. Besides, using stereologic technique the cilia length and cilia density of endosalpinx were studied in 50 cases through image analysis system. Results showed that except for a significant higher frequency of ovarian pregnancy in IUD-users, there was no significant differences between the 2 groups. It indicated that the degree of injury of fallopian tubes and the factors causing the damage were similar in both groups. The authors concluded that IUD does not increase the risk of fallopian tube lesions.

Fallopian Tubes↗

PubMed1992

[Balloon dilatation for prostatic obstruction].

Balloon catheter dilatation of the prostate (BCDP) was performed in 25 patients with prostatic hypertrophy. All patients suffered from dysuria or urinary retention. Follow-up for 8-14 months showed that 20 patients had satisfactory results. The balloon catheter of 25 mm x 50 mm was inflated at 4-6 atm for 10-15 minutes. BCDP was modified for the use in the local hospitals.

Aged↗

PubMed1992

[The stereological study on normal and intrauterine growth retardation (IUGR) placentas].

The stereological study was carried out in 8 normal and 22 IUGR placentas through Image Analysis System of Cambridge Instruments Quantimet 970 and 10 indices were analysed. The results showed that in the IUGR group the placental indices of total surface area of acroteric villus, total volume of intervillous space, volume density of fetal capillaries, total volume of fetal capillaries and surface area density of vasculo-syncytial membrane were significantly reduced than those in the normal group. The vasculo-syncytial membrane became significantly thick in the IUGR placental group. It was confirmed that a close relation exists between IUGR and placental quantitative changes. The major pathological findings of the placenta were prematuration and hypoplasia.

Anthropometry↗

PubMed1991

[Scanning electron microscopic observations on Clonorchis sinensis cercariae].

The surface structure of Clonorchis sinensis cercariae was observed under scanning electron microscope. The cercaria was of the parapleuro-lophocercous type. The measurements of the cercariae were as follows: body 137-240 x 62-90 microns, tail 320-470 x 21-34 microns, oral sucker 27-46 x 23-33 microns. The ventral sucker was incompletely developed and much smaller than the oral one. The whole body surface was covered with backward pointing spines. Four horizontal rows of oral spines were found around the mouth opening of the oral sucker. These spines diminished in size with the distance of the rows from the opening and their numbers were 12, 18, 19 and 18 respectively. Transverse and longitudinal plicae formed from tegument were found on the surface of the tail, being in connection with dorsal and ventral fins along posterior half of the tail. Many sensory structures or papillae were observed on the body. They were of three different types: 1) papillae with long or short cilia distributed widely on the body surface and around the mouth opening, the number of papillae with cilia on the body surface being greater than that described by Komiya et al (1940); 2) a few sensory fossa on the tail, measuring 1.0 x 0.6 microns; 3) a few ring-type papillae with knob on the body surface with pore of 0.5 micron in diameter.

Animals↗

PubMed1990

[Ultrastructural changes in the body wall and gut of Clonorchis sinensis in rats after albendazole treatment].

The effect of albendazole on the body wall and gut of Clornorchis sinensis was studied with transmission and scanning electron microscopes after albendazole administration to rats infected with Chonorchis sinensis at a single dose of 150 mg/kg. The results showed that swelling and adhesion of the projections of the tegument and gut microvilli occurred 1h after medication. Necrosis and disruption of the projections and the gut microvilli were seen at the 24th h. By the 72nd h, detachment of the partial projections were seen. The dynamic process of the damages observed on the tegument was identical with that of the gut microvilli (Figs. 1-10).

Albendazole↗

PubMed1989

Studies on circulation rate and flow model for external loop bioreactor.

We performed experiments in an external loop bioreactor 250 mm in diameter and 4500 mm in height. The variation in circulation rate Um was studied over a wide range of surface gas velocities (UG = 0-24.9 cm/s), and a bioreactor flow model was obtained. We developed a new method (i.e., an electrical conductivity tracking system) to measure circulation rate and solve the problem of measuring general circulation resistance. A theoretical formula for Um was derived for use in scaling up the bioreactor: [formula: see text]

Biotechnology↗

PubMed1986