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Biomedical subjects

B Zupańska

Publications and source records attributed to B Zupańska.

At least 19 recordsLinked to original sources
PubMed1991

[Pseudothrombocytopenia].

Pseudothrombocytopenia in four patients has been described. It was due to platelet "++EDTA-dependent" antibodies. The number of platelets was described only in EDTA blood while the number was normal when platelet were evaluated in citrate blood. The importance of distinguishing pseudothrombocytopenia from thrombocytopenia is discussed, especially in view of unnecessary treatment, delayed operation etc.

Adult↗
PubMed1990

Rosetting, phagocytosis and immune red cell damage.

Observations on rosette formation of red blood cells sensitized with a known number of IgG1 or IgG3 polyclonal or monoclonal anti-Rh(D) antibodies with monocytes, lymphocytes and granulocytes have provided further evidence for the essential role of monocytes/macrophages in red cell destruction. The rosette test with monocytes and the monocyte-monolayer assay (MMA) have confirmed, on a quantitative basis, the greater binding ability of IgG3 than of IgG1 antibodies and that the rate of interaction with red cells increases in proportion to the level of their sensitization. These observations have suggested that the MMA may reflect haemolysis in vivo. It has appeared that the MMA is suitable for assessing haemolysis in many cases of haemolytic disease of the newborn, in autoimmune haemolytic anaemia and in patients with alloantibodies to be transfused. Since in individual cases a clear correlation between the MMA results and haemolysis in vivo was not observed, the factors which may influence such discrepancies are discussed.

Erythrocytes↗
PubMed1978

Automated screening of red cells for the detection of autoantibodies.

The AutoAnalyser Technicon II system was adapted for screening red cells for the presence of warm-type autoantibodies. A significant decrease in optical density was recorded when the red cells of 38 patients with autoimmune haemolytic anaemia were tested using the bromelin-methylcellulose method. This was never seen with the red cells of 226 patients with other diseases or of healthy blood donors. The method is very simple and more sensitive than the manual direct antiglobulin test.

Anemia, Hemolytic, Autoimmune↗
PubMed1976

Autoimmune haemolytic anaemia in children.

Forty-four children with autoimmune haemolytic anaemia (AIHA) are described: 31 had acute, subacute or chronic disease with warm autoantibodies and 13 had acute or chronic anaemia with cold autoantibodies. The commonest forms were the acute and subacute types with warm autoantibodies and these were more frequent in young children, while chronic AIHA occurred mainly among children at puberty. In about 16% the anaemia was accompanied by a chronic disorder but in over 50% the anaemia was preceded by an acute infection or immunization. The former gave rise mainly to chronic anaemia, but the latter was associated with the acute and subacute forms. In general the prognosis was good and death was never caused by anaemia per se. The prognosis was worse in patientw with clinical features of thrombocytopenia and bleeding and with the immunological findings of free autoantibodies in the serum and a positive direct antiglobulin test. In acute and subacute forms, treatment with corticosteroids and sometimes with blood transfusions was effective. In chronic forms of the disease it was often necessary to give additional immunosuppressive drugs or/and to perform a splenectomy.

Acute Disease↗
PubMedDate not supplied

[Detection of light chains of warm-type autoantibodies in autoimmune hemolytic anemia with the aid of an autoanalyzer].

A method was evolved for detection of light chains of erythrocyte autoantibodies using a Technicon II AutoAnalyser with properly selected dilutions of bromelin and methylcellulose and anti-kappa and anti-lambda sera. It was demonstrated that the method is superior to the direct Coombs test because it made possible detection of one or both light chains in warm autoantibodies coating the erythrocytes of 20 patients with autoimmunohaemolytic anaemias in whom they could not have been detected by the manual method. It was found that in warm-type autoantibodies light chains can be detected always and that in some cases only one light chain is found.

Anemia, Hemolytic, Autoimmune↗
PubMedDate not supplied

[Effect of HL-A system antigen differences on the specificity of produced anti-leukocyte antibodies].

In Rh-negative volunteers immunized for development of anti-D antibodies the specificity of developed anti-leucocyte antibodies was determined. The specificity of these antibodies was analysed in relation to HL-A antigenic differences determined in donors and recipients. The antigenic power of sublocus II was found to be superior to that of sublocus I. Another observation was more frequent production of cross-reacting antibodies. A trial of analysis of these volunteers in whom no antileucocyte antibodies developed during immunization with blood was undertaken.

Antibody Formation↗
PubMedDate not supplied

[HL-A antigens in patients with Hodgkin's disease].

HL-A antigens were determined in 46 patients with Hodgkin's disease and a statistical analysis was carried out by the chi square test to compare the incidence of these antigens in patients with that in healthy people. Statistically significant differences were found in the first place in the HL-A5 antigen (chi square = = 32.2) (p less than 0.005). This antigen was much more frequent in patients (43%) than in the healthy population (15%). A slightly lower frequency of antigen W5 was found in the group of patients (healthy controls -- 13.5%, patients -- 4.5%, chi square = 7.08, p less than 0.05).

Histocompatibility Antigens↗
PubMedDate not supplied

[Alloimmune neonatal granulocytopenia caused by anti-NA1 antibodies].

Two cases of neonatal alloimmune granulocytopenia due to fetomaternal incompatibility with granulocyte-specific antigen NA1 are presented. The diagnosis was based on the detection of anti-NA1 alloantibodies in maternal and neonates sera in leukoaglutination and immunofluorescence tests with granulocytes. Both children (twins) had severe granulocytopenia and unfortunately died.

Agranulocytosis↗
PubMedDate not supplied

[Prediction of the severity of fetal erythroblastosis using the erythrocyte phagocytosis test and evaluating anti-Rh antibody titers and their levels by autoanalysis].

Erythrophagocytosis++ test was performed in 45 pregnant Rh-negative women together with routine anti-Rh antibodies assay with indirect antiglobulin test and their concentration determined in an autoanalyser. It was found that the degree of ++phagocytosis of the standard erythrocytes immunized with maternal anti-Rh antibodies by the monocytes from healthy subjects is an important predictive factor enabling to foresee a severity of hemolytic disease in newborn babies. ++Phagocytosis over 50% predicts a severe course of the disease whereas under 20%--a mild form of the serologic conflict.

Autoanalysis↗
PubMedDate not supplied

[Autoimmune hemolytic anemia with presence of 3 types of autoantibodies to erythrocytes].

In a patient with chronic lymphatic leukaemia severe autoimmunohaemolytic anaemia (NAIH) was observed with coexistence of cold incomplete IgM, warm incomplete IgG and warm haemolysins active against papainized erythrocytes in low pH medium. The serologic and immunochemical characteristics of autoantibodies are presented and the mechanism of their action in vitro is tentatively explained.

Anemia, Hemolytic, Autoimmune↗