Biomedical subjects

Bahram Parvin

Publications and source records attributed to Bahram Parvin.

3 recordsLinked to original sources

PubMed2006

Imaging features that discriminate between foci induced by high- and low-LET radiation in human fibroblasts.

In this study, we investigated the formation of radiation-induced foci in normal human fibroblasts exposed to X rays or 130 keV/mum nitrogen ions using antibodies to phosphorylated protein kinase ataxia telangiectasia mutated (ATMp) and histone H2AX (gamma-H2AX). High-content automatic image analysis was used to quantify the immunofluorescence of radiation-induced foci. The size of radiation-induced foci increased for both proteins over a 2-h period after nitrogen-ion irradiation, while the size of radiation-induced foci did not change after exposure to low-LET radiation. The number of radiation-induced ATMp foci showed a more rapid rise and greater frequency after X-ray exposure and was resolved more rapidly such that the frequency of radiation-induced foci decreased by 90% compared to 60% after exposure to high-LET radiation 2 h after 30 cGy. In contrast, the kinetics of radiation-induced gamma-H2AX focus formation was similar for high- and low-LET radiation in that it reached a plateau early and remained constant for up to 2 h. High-resolution 3D images of radiation-induced gamma-H2AX foci and dosimetry computation suggest that multiple double-strand breaks from nitrogen ions are encompassed within large nuclear domains of 4.4 Mbp. Our work shows that the size and frequency of radiation-induced foci vary as a function of radiation quality, dose, time and protein target. Thus, even though double-strand breaks and radiation-induced foci are correlated, the dynamic nature of both contradicts their accepted equivalence for low doses of different radiation qualities.

Ataxia Telangiectasia Mutated Proteins↗

PubMed2003

Ionizing radiation induces heritable disruption of epithelial cell interactions.

Ionizing radiation (IR) is a known human breast carcinogen. Although the mutagenic capacity of IR is widely acknowledged as the basis for its action as a carcinogen, we and others have shown that IR can also induce growth factors and extracellular matrix remodeling. As a consequence, we have proposed that an additional factor contributing to IR carcinogenesis is the potential disruption of critical constraints that are imposed by normal cell interactions. To test this hypothesis, we asked whether IR affected the ability of nonmalignant human mammary epithelial cells (HMEC) to undergo tissue-specific morphogenesis in culture by using confocal microscopy and imaging bioinformatics. We found that irradiated single HMEC gave rise to colonies exhibiting decreased localization of E-cadherin, beta-catenin, and connexin-43, proteins necessary for the establishment of polarity and communication. Severely compromised acinar organization was manifested by the majority of irradiated HMEC progeny as quantified by image analysis. Disrupted cell-cell communication, aberrant cell-extracellular matrix interactions, and loss of tissue-specific architecture observed in the daughters of irradiated HMEC are characteristic of neoplastic progression. These data point to a heritable, nonmutational mechanism whereby IR compromises cell polarity and multicellular organization.

Cadherins↗

PubMed2003

Harmonic cut and regularized centroid transform for localization of subcellular structures.

Two novel computational techniques, harmonic cut and regularized centroid transform, are developed for segmentation of cells and their corresponding substructures observed with an epi-fluorescence microscope. Harmonic cut detects small regions that correspond to small subcellular structures. These regions also affect the accuracy of the overall segmentation. They are detected, removed, and interpolated to ensure continuity within each region. We show that interpolation within each region (subcellular compartment) is equivalent to solving the Laplace equation on a multi-connected domain with irregular boundaries. The second technique, referred to as the regularized centroid transform, aims to separate touching compartments. This is achieved by adopting a quadratic model for the shape of the object and relaxing it for final segmentation.

Algorithms↗