Bai-lin Liu | PubMed Health

PubMed2003

[Preliminary analysis on human telomerase reverse transcriptase gene expression in acute leukemia].

OBJECTIVE: To explore the relationship between gene expression of human telomerase reverse transcriptase (hTERT) and its clinical characteristics in leukemia. METHODS: The protocol of RT-PCR was used to detect the hTERTmRNA expressing levels in peripheral blood samples from leukemic patients under primary treatment(n=42), in complete remission(n=21), with recurrent leukemia (n=4); and from normal subjects (n=5), respectively. RESULTS: The positive percentage of hTERTmRNA expression was 73.81% for the primary treatment cases, and 19.05% for the complete remission cases. All of the recurrent cases gave positive results. One of the normal controls presented low level of hTERTmRNA expression. The expressing level of hTERTmRNA in primary treatment cases was 0.64+/-0.21, in complete remission leukemia 0.31+/-0.16, in recurrent cases 0.84+/-0.09, and in normal controls 0.10. CONCLUSION: The activation of telomerase may be an essential factor in the development of leukemia and usually be the late event in its progression. As an indicator of leukemia cell, the detection of hTERT mRNA may be used in clinical analysis, disease monitoring and prognosis judgement.

Acute Disease↗

PubMed2003

[Circadian rhythms of DNA synthesis and telomerase expression in hepatic cancer transplanted in nude mice].

OBJECTIVE: To study the circadian rhythms of DNA synthesis and telomerase expression in hepatic cancer transplanted in nude mice. METHOD: Sixteen BALB/C mice were synchronized with an alternative lighting regimen with 12 h for light and 12 h for darkness (12:12 LD) for 4 weeks. Hepatic cancer cells (SMMC-7721) were implanted into both flanks of each mouse. One week after transplantation, sampling from the tumor was conducted at 3, 9, 15 and 21 h after light onset (HALO). Single cell suspension was obtained and stained with propidium iodide. The cellular DNA content was measured by flow cytometry. Telomerase activity was measured by PCR-ELISA assay. Data were documented by ANOVA and Cosinor analysis. RESULT: The proportion of tumor cells in phase G1, S, G2/M and telomerase activity varied according to circadian time with statistical significance, and the telomerase activity showed a synchronized variation. The distribution curves of both phase S and the expression level of telomerase were fit for Cosinor changes. CONCLUSION: DNA synthesis and telomerase expression of SMMC-7721 cells transplanted into the nude mice varies according to the circadian rhythm. The results provide a guidance for laying down the chemotherapy protocol for human tumors, especially when the telomerase inhibitor was used as the anti-cancer agent.

Animals↗