Biomedical subjects

Baoguo Jiang

Publications and source records attributed to Baoguo Jiang.

7 recordsLinked to original sources

PubMed2006

Study on small gap sleeve bridging peripheral nerve injury.

Epineurium or perineurium neurorrhaphy to recover the nerve continuity was the choice of peripheral nerve mutilation. The nerve selective regeneration theory was put forth by Cajal et al. As this theory was gradually accepted, many researchers had focused on it and its possible application. Our labs had centered on the small gap sleeve bridging fields for about 30 years, using autogeneic vein, artery and biogradable chitin conduits. Our goal was to improve the nerve regeneration effect by means of nerve selective regeneration theory and degradable biomaterials. This serial experiment was to confirm the possibilities of using conduit small gap sleeve bridging to substitute the traditional epineurium neurorrhaphy.

Animals↗

PubMed2006

The biocompatibility research of functional Schwann cells induced from bone mesenchymal cells with chitosan conduit membrane.

To explore the adhesion and proliferation characteristics of bone marrow mesenchymal cells (MSCs) to chitosan conduit membrane, MSCs were induced by a sequential composition Beta-mercaptoethanol, retinoic acid, forskolin, basic-FGF, PDGF and heregulin. Schwann Cell markers, namely S-100 and GFAP, were used to discriminate the induced MSCs' properties by immunofluorescent staining. These results suggested that MSCs can take on Schwann cell's phenotype in vitro and the induce MSCs were gifted with good biocompatibility to biogradable chitosan conduit membrane. The results provided the possibilities to using the induced MSCs with chitosan conduit membrane in artificial peripherial nerve fields to promote nerve regeneration.

Animals↗

PubMed2005

Bridging small-gap peripheral nerve defects using biodegradable chitin conduits with cultured schwann and bone marrow stromal cells in rats.

Nerve regeneration requires not only an autologous, allogenous, or biodegradable scaffolding, but additional interactions with regeneration-promoting Schwann cells. Considering the pluripotency of bone marrow stromal cells into different lineages, the authors compared biodegradable conduits with the application of cultured Schwann cells and bone marrow stromal cells in a rat sciatic injury model. Simple conduit bridging served as controls. Electrophysiologic evaluation and histologic morphometrical analysis were performed after 6 weeks; both groups with cultured cells showed a statistically significantly higher number of axons, more well-shaped remyelinated axons, and an advance in clinical functional recovery (SFI) than the simple conduit-bridging group. Confocal microscopy found that bone marrow stromal cells adopted the Schwann-cell phenotype, expressing S100 protein. Considering the ease of aspiration and greater resource of bone marrow stromal cells, the implantation of a biodegradable conduit with cultured bone marrow stromal cells was capable of presenting an alternative to conduits with Schwann cells for bridging nerve defects.

Absorbable Implants↗

PubMed2004

Accurate determination of isthmus of lumbar pedicle: a morphometric study using reformatted computed tomographic images.

STUDY DESIGN: Lumbar pedicle isthmus, the narrowest section of pedicle, was investigated. OBJECTIVE: To determine the lumbar pedicle isthmus accurately and measure related parameters. SUMMARY OF BACKGROUND DATA: Accurate anatomic knowledge of pedicle parameters is critical for a successful transpedicular procedure. Many studies on lumbar pedicle dimensions have been conducted, yet less is known about how to obtain these data and their veracity. In fact, the narrowest section is present in the pedicle, i.e., the isthmus of the pedicle. This is the bottleneck of the pedicle; to determine and measure it could illustrate the true morphologic characteristics of the lumbar pedicle. METHODS: Ultra high-speed spiral CT scan of lumbar spine was performed in 41 patients of Chinese origin. After reformation of the original images, isthmus and transverse plane of pedicle axis were determined. Eleven dimensions of isthmus plane and three dimensions of transverse plane were calculated using software. RESULTS: Pedicle endosteal width and height in female patients who were over the age of 50 were greater than those of female patients younger than 50 years old. The isthmus endosteal width from L1 to L5 was 5.2, 6.0, 7.5, 7.5, and 8.7 mm and 4.0, 4.1, 5.4, 5.7, and 7.1 mm, respectively, in men and women. Isthmus inclination inclined to midline with less than 10 degrees above L3 but increased in L4 and 30 degrees in L5. CONCLUSIONS: An objective narrowest section, the pedicle isthmus, exists in lumbar pedicle axis. The parameters of isthmus, especially isthmus endosteal width, show the morphologic characteristics of the lumbar pedicle and are the most important data provided for transpedicular procedures. The small pedicles of female patients over the age of 50 also show certain primary osteoporosis. Isthmus inclination puts the pedicle in a more complicated space position. A better understanding of the complicated structure of the pedicle isthmus guarantees success in transpedicular procedures.

Adult↗

PubMed2004

Distal nerve elongation vs nerve grafting in repairing segmental nerve defects in rabbits.

Segmental nerve defects have been repaired with nerve grafts. However, regenerating fibers must go through two coaptation sites, and donor site morbidities are inevitable. We elongated the distal nerve segment using a tissue expander and reconnected the nerve. We compared these results with those of nerve grafting. Nerve injury models were created in the median nerves of both forelimbs in 27 rabbits. The right nerve was repaired by elongation, and the left by tibial nerve grafting. The length of the elongation was the same as that of the graft in each animal. Eight rabbits had a 10-mm segment repaired, 9 had a 15-mm segment repaired, and 10 had a 20-mm segment repaired. Evaluated outcomes were nerve conduction velocity, contractile force of the flexor muscle, axons number, and fiber diameter. The elongated and grafted groups did not differ substantially on any outcome. Distal nerve elongation could be an alternative to nerve grafting for repairing large nerve defects.

Analysis of Variance↗

PubMed2004

Bone marrow stromal cells differentiated into functional Schwann cells in injured rats sciatic nerve.

Bone marrow stromal cells (MSCs) have been shown to differentiate into various lineage cells including neural cells in vitro and in vivo. We therefore examined whether MSCs can differentiate into Schwann cells in injured peripheral nerves, After cultured in vitro, PKH-67-labeled MSCs were injected into the mechanically injured rat sciatic nerves. Three weeks after injection, immunofluorescent examinations were carried out. MSCs had been incorporated around the injured nerves and differentiated into Schwann cells. MSCs had accumulated mainly in the epineurium around the injured nerve. The incorporated cells partially expressed GFAP, S-100, and P75. These results confirmed the possibility that MSCs have the ability to differentiate into Schwann cells, and that injection of MSCs into the injured peripheral nerve would help repair damaged nerve.

Animals↗

PubMed2002

Compound injection of radix Hedysari to promote peripheral nerve regeneration in rats.

OBJECTIVE: To study the effect of compound injection of Radix Hedysari on peripheral nerve regeneration in rats. METHODS: Seventy-five healthy adult SD male rats, weighing 150 g, were randomized into 5 groups (15 rats in each group). The bilateral sciatic nerves of the rats were exposed and clamped with a smooth clamp to make an injury area of 2 mm. After clamp operation Group 1 was injected with compound injection of radix Hedysari (CIRH) 1.5 ml/day, Group 2 with CIRH 1.0 ml/day, Group 3 with CIRH 0.5 ml/day, Group 4 with nerve growth factor (NGF) 50 U/day, and Group 5 was taken as the control group without any management. The bilateral sciatic nerve was taken out at 3 days, 1, 2 and 4 weeks after clamping, stained with osmic acid and observed microscopically. The myelinated nerve fibers were counted. The nerve conduction velocity was determined 2 and and 4 weeks before sample taking the sciatic nerve function index was measured 4 weeks before sample taking. RESULTS: The results of nerve conduction velocity, the myelinated nerve fiber count and the sciatic functuion index in the CIRH treated groups were better than those in the control group. The results of the nerve conduction velocity and the myelinated nerve fiber count at 2 weeks and the nerve conduction velocity at 4 weeks in Group 1 were better than those of Group 4. Biological observation showed that degenerated and necrotic myelin sheath in CIRH treated Groups at 2 and 4 weeks decreased remarkably compared to the NGF treated group. CONCLUSIONS: CIRH can promote regeneration of peripheral nerves and absorption of degenerated and necrotic injured nerves. It has the same effect as NGF.

Animals↗