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Biomedical subjects

Barbara Rogala

Publications and source records attributed to Barbara Rogala.

26 records · Page 2Linked to original sources
PubMed2003

[Immunophenotype of peripheral blood mononuclear cells in atopic diseases].

According to the different theories considering the role of CD30 antigen in the differentiation of T lymphocytes, the aim of this study was to analyze the immunophenotype of peripheral blood mononuclear cells (PBMC) in patients with mild atopic asthma and seasonal allergic rhinitis in out of season period. The immunophenotype of peripheral blood mononuclear cells (CD4, CD38, CD8, CD23, CD25, CD57, CD30, HLA-DR) was analyzed by flow-cytometry. A statistically significant rise in the percentage of CD30+ PBMCs in asthma group (3.50 +/- 2.07%) was seen when compared with allergic rhinitis group (1.80 +/- 0.90%). In allergic rhinitis patients a significant rise in the percentage of CD8+ lymphocytes (13.21 +/- 5.87%) was observed in comparison to asthma group (8.36 +/- 5.88%). No statistically significant differences in the expression of the other estimated molecules were detected. The enhancement of CD30 molecule expression on PBMCs in symptomatic asthma is supposed to confirm, due to Romagnani's theory, the predominance of Th2-type response in the pathogenesis of immunoinflammation active phase. PBMCs from patients with allergic rhinitis in out-of-season period may switch into cytotoxic/suppressive cells, confirmed by rise in the percentage of CD8+.

Adolescent↗
PubMed2002

[The role of nitric oxide in pathogenesis of essential hypertension].

It is known that NO is involved in a variety of physiological functions including the regulation of blood pressure. Dysregulation of L-arginine/NOS/NO biological activity occurs in blood hypertension. The present review will focus on a significance of NO in pathogenesis of essential hypertension.

Arginine↗
PubMed2002

[Endothelin-1 and bradykinin in nasal lavage fluid of patients allergic to tree pollen].

Hay fever is one of the most frequent upper airway diseases. The nasal epithelium is considered to play and active role in the allergic inflammation through its capacity to synthesize and release a wide range of cytokines and mediators. One substance of potential relevance in this regard is endothelin (ET). We postulated that ET-1 could contribute to pathomechanism of seasonal allergic rhinitis. The aim of this study was to demonstrate release of this peptide and bradykinin into the nasal lavage fluid (NLF) of tree pollen allergic patients in response to allergen challenge. 16 subjects aged 25.5 +/- 3.3 years with clinical history of tree pollen allergic rhinitis participated in the study. Nasal allergen provocation with following lavage was performed in all subjects. Ten of them were hyposensitized preseasonally by allergic vaccine Allergovit (Allergopharma, Germany). Six patients received treatment with antihistamines, cromoglicates or steroids. Bradykinin and endothelin-1 in the nasal secretion were assayed before starting immunotherapy and early during tree pollen season. Increase in concentration of bradykinin in nasal lavage fluid from hyposensitized patients were significantly lower than from second group, 509.4 +/- 110.4 before 942.9 +/- 234.89 pg/ml after allergen provocation versus 417.8 +/- 100.4 and 2001.6 +/- 223.6 pg/ml (p < 0.05). We noticed lower concentration of ET-1 in NLF of hay fever patients, 17.5 +/- 3.9 versus 13.9 +/- 2.7 pmol/ml. We conclude that bradykinin and endothelin-1 could be involved in the pathogenesis of hay fever.

Adult↗
PubMed2002

Do the molecules CD26 and lymphocytes activation gene-3 differentiate between type 1 and 2 T cell response?

BACKGROUND: The Th1/Th2 paradigm is considered to be responsible for the development of many immunological disorders, including atopic diseases and diabetes mellitus type 1. So far, however, no unequivocal markers identifying the two subpopulations of T cells have been found. OBJECTIVE: To examine the expression of two putative markers of Th1-mediated disorders, CD26 and lymphocytes activation gene-3 molecules, on CD4+ and CD8+ lymphocytes in the peripheral blood of atopic and diabetic donors. METHODS: 11 patients (9 males/2 females, age range: 21-29, median age: 23) suffering from episodic atopic bronchial asthma (AO group) and 11 patients (9 males/2 females, age range: 33-47, median age: 42) with insulin-dependent diabetes mellitus (DM group) were included into the study. Atopy was excluded in all diabetic patients. The following T cell subsets were analyzed by flow cytometry (FAC-Scan): CD8+CD26+, CD4+CD26+, CD4+LAG-3+, CD8+LAG-3+, and CD56+. Nonparametric tests were used for statistical analysis, and results were expressed as median and quartile range. RESULTS: Among all analyzed T cell subsets, the number of CD4+LAG-3+ and CD8+LAG-3+ cells was significantly higher in the A0 group--8.3% (5.4-11.7) vs DM 5.4% (3.9-5.9), p < 0.05, and 13.3% (8.8-19) vs DM: 7.1% (6.2-8.3), p < 0.008, respectively. The ratio between CD4+/CD8+ subsets was similar in both groups: CD4+CD26+/CD8+CD26+--AO: 7.2 (5.3-10.1); DM: 6.4 (5.5-12.6), p = 0.77 and CD4+LAG-3+/CD8+LAG-3+--AO: 0.64 (0.58-0.67); DM: 0.71 (0.64-0.78), p = 0.082. CONCLUSION: The results suggest that the two examined putative markers of type 1 T cells do not discriminate between Th1- and Th2-dominated responses.

Adult↗
PubMedDate not supplied

Pruritic urticarial papules and plaques of pregnancy.

Pruritic urticarial papules and plaques of pregnancy (PUPPP) are among the most common pruritic dermatoses observed in pregnant women. PUPPP appears most frequently in the third trimester, in primigravidas, and in multiple gestation pregnancies. The eruption of changes occurs initially on the abdomen and extends over the thighs, legs, back, buttocks, arms, and breasts. Skin changes typical for PUPPP are erythematous, urticarial plaques, and papules. Rash regression is usually observed within 6 weeks postpartum. Immunologic mechanisms, hormonal abnormalities, and abdominal skin distension have been suggested as etiologic mechanisms. PUPPP is thought to be harmless for the mother and fetus and usually requires intervention only for symptom relief. In some cases, laboratory investigation, histologic examination, and immunologic study should be performed to exclude more serious disorders of pregnancy, such as herpes gestationis or intrahepatic cholestasis of pregnancy. This article reviews the epidemiology, clinical manifestation, etiology, differential diagnosis, and treatment of PUPPP.

Diagnosis, Differential↗
PubMedDate not supplied

[Symptoms of anxiety and depression in atopic eczema/dermatitis syndrome].

UNLABELLED: The presence of chronic disease is a risk factor for the development of mood disturbances and panic disorders. They can influence the course of disease and effectiveness of therapy. Depression may be the cause of making light doctor's advice. Anxious patients often aggravate symptoms of the disease. AIM: To study symptoms of anxiety and depression in patients suffering from atopic eczema/dermatitis syndrome (ZAZS). Material. We studied 38 patients suffering from adequately controlled moderate ZAZS and 62 volunteers in the control group. METHODS: Mental status of subjects was assessed by means of State and Trait Anxiety Inventory (STAI) and Beck Depression Inventory (BDI) questionnaires. RESULTS: ZAZS patients demonstrated higher intensity of anxiety (as a trait and as a state) than healthy subjects. Intensity and prevalence of depression in the atopic eczema/ dermatitis syndrome group was higher than in the control group. CONCLUSIONS: Patients suffering from atopic/eczema dermatitis syndrome are pre-disposed to anxiety and depression manifestation. Even adequately controlled symptoms of atopic/eczema dermatitis syndrome may be the cause of those disturbances' occurrence.

Adaptation, Psychological↗