Biomedical subjects

Bei Liu

Publications and source records attributed to Bei Liu.

23 records · Page 2Linked to original sources

PubMed2003

Preparation of humanized ovarian carcinoma anti-idiotypic minibody.

Murine anti-idiotypic monoclonal antibody (MAb) 6B11 mimicking the tumor-associated antigen OC166-9 is used as a vaccine for the induction of an anti-tumoral immunity in experiments of in vitro and in vivo animal model with ovarian carcinoma. In this article, we have humanized 6B11 anti-idiotypic minibody using overlap polymerase chain reaction (PCR) and DNA recombinant technique, prokaryotic expression vector was produced by genetic fusion of 6B11V(L)-V(H) to human IgG1 hinge and CH3 region. Transformed E. coli BL21(DE3) were propagated and induced by isopropyl-beta D-thiogalactopyranoside (IPTG). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) showed that a protein band with molecular weight of 50kD appeared as the expected size after transformation. Molecular weight of 100 kDa may be examined by electrophoresis in nondenaturing systems. The fusion protein was analyzed with enzyme-linked immunosorbant assay (ELISA), inhibition ELISA tests and Western blot, respectively. The humanized anti-idiotype minibody showed capacity of bivalent binding to ovarian cancer MAb COC166-9 and goat anti-human immunoglobulin IgG1. It is useful reagents for clinical use.

Animals↗

PubMed2002

An integrated view of the roles and mechanisms of heat shock protein gp96-peptide complex in eliciting immune response.

Heat shock protein (HSP) gp96, or grp94 is an endoplasmic reticular (ER) paralog of the cytosolic HSP90. Being abundant and non-polymorphic, gp96 plays significant roles in maintaining protein homeostasis in the secretory pathway. This "house-keeping" role of gp96 has now been overshadowed by the intriguing findings that gp96 modulates both the innate and adaptive components of the immune system. It has been found that, (i) gp96 is one of the major peptide binding proteins in the ER, (ii) gp96 interacts specifically with receptors including CD91 and possibly toll-like receptors (TLRs), on the surface of professional antigen presenting cells (APCs), (iii) interaction with APCs leads to re-presentation of gp96-chaperoned peptides to the major histocompatibility complex (MHC) molecules of APCs, (iv) direct access of gp96 to APCs triggers functional activation of APCs. In this review, we will examine each of these immunological attributes of gp96 critically. As experimentalists, we will also propose specific experiments to examine the argument that gp96, perhaps along with other members of HSP family, is the antigenic carrier for mediating cross priming of antigen-specific T lymphocytes in vertebrates.

Amino Acid Sequence↗

PubMed2002

Overcoming immune tolerance to cancer by heat shock protein vaccines.

Heat shock proteins (HSPs) are a large family of inducible but also ubiquitously and constitutively expressed protein chaperones involved in assisting protein folding and unfolding in the cells. The realization of the immunological significance of HSPs came from the observation that tumor cell-derived HSPs could immunize against tumors, although there were no structural differences between HSPs from normal cells and from cancer cells. Studies of this puzzle have uncovered two unique immunological properties of HSPs. One is their ability to associate and present antigenic fingerprints/peptides of cells to MHC antigens. The other is their ability to activate dendritic cells (DCs), which are the most efficient antigen-presenting cells. These surprising immunological attributes of HSPs are now the basis for a number of already completed clinical trials for cancer immunotherapy. Because DCs orchestrate both innate and adaptive immunity, the findings that HSPs can modulate the functions of DCs may have fundamental implications. We hypothesize that HSPs are at the forefront in counterbalancing T-cell tolerance to tumor-associated antigens and thus play pivotal roles in antitumor immunity in vivo.

Cancer Vaccines↗

PubMed2002

[The observation of clinical efficacy of combined modality therapy in 58 cases of perennial allergic rhinitis].

OBJECTIVE: To study the effect of combined modality therapy on perennial allergic rhinitis (PAR). METHOD: Fifty-eight cases of PAR received a 4-weeks treatment with combined beclomethasone dipropionate nasal spray (BDP), cetirizine hydrochloride, ethmoidal nerve radiofrequency treatment, and half-dose combined BDP, cetirizine hydrochlonde was used for improving the recurrence symptoms in one year follow-up. RESULT: The overall effective (excellent/good) rate was 94.83% (excellent 72.41%, good 22.41%). The recurrence rate was 51.72%, but symptoms of 27 recurrence cases were lighter than pre-treatment. All treatments were well tolerated, and no serious adverse events occurred. CONCLUSION: The reasonable combined modality therapy is aimed at the complicated pathogenesis of PAR, and the curative effect is satisfactory, so it is a good way to treat PAR.

Administration, Intranasal↗

PubMed2002

[Clinical study of non-invasive nasal sinus mycoses].

OBJECTIVE: To explore the pathogeny, diagnosis and treatment of the noninvasive nasal sinus mycoses. METHOD: 11 cases with nasal sinus mycoses were analyzed retrospectively. All of them had undergone endoscopic operation. RESULT: After operation all cases got regular check up under endoscope. 9 cases recovered quickly. The other 2 had caseous matter in their maxillary sinus in the initial stage. After cleaning up the fungus ball and washing the maxillary sinus for several times, the surgical cavity became clear. 10 cases had been followed up for 6 months to 5 years and no recurrence occurred. The other one lost follow-up. CONCLUSION: Endoscopic operation is the basic surgical method to treat the non-invasive nasal sinus mycoses. Excising the focus thoroughly, correcting the abnormal nasal cavity structure and resuming the cleanout function of nasal sinus cilia are the principle of treatment.

Adult↗