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Biomedical subjects

Bernard Bodo

Publications and source records attributed to Bernard Bodo.

At least 19 recordsLinked to original sources
PubMed2006

Antimicrobial activity of the methanolic extracts and compounds from Vismia laurentii De Wild (Guttiferae).

Methanolic extracts prepared from the leaves, twigs and the roots of Vismia laurentii De Wild as well as nine compounds isolated from these crude extracts, were tested for their antimicrobial activity against Gram-positive bacteria (six species), Gram-negative bacteria (12 species) and two Candida species using disc diffusion and well micro-dilution methods. The disc diffusion assay indicated that the crude extract was active against all the pathogens tested, whereas isolated compounds showed selective activities. The degree of sensitivity of the test organisms to purified compounds varied from 25 to 90%. Fridelin (8) was found to be the most active compound, while Bivismiaquinone (3) was the least active. The lowest minimum inhibition concentration (MIC) values as obtained by the micro-dilution assays were 19.53 and 1.22 microg/ml for the crude extracts and purified compounds, respectively. The lowest value for the purified compounds (1.22 microg/ml) was obtained with O(1)-demethyl-3',4'-deoxypsorospermin-3',4'-diol (6) on Candida gabrata and Bacillus subtilis; 1,8-dihydroxy-6-methoxy-3-methylanthraquinone (5) on Bacillus subtilis and 6-deoxyisojacareubin (7) on Bacillus stearothermophilus. These results provide promising baseline information for the potential use of these crude extracts as well as some of the isolated compounds in the treatment of bacterial and fungal infections.

Anti-Infective Agents↗
PubMed2006

Antimalarial and cytotoxic activities of ethnopharmacologically selected medicinal plants from South Vietnam.

Malaria is a major global public health problem and the alarming spread of drug resistance and limited number of effective drugs now available underline how important it is to discover new antimalarial compounds. An ethnopharmacological investigation was undertaken of medicinal plants traditionally used to treat malaria in the South Vietnam. Forty-nine plants were identified, 228 extracts were prepared and tested for their in vitro activity against Plasmodium falciparum, and assessed for any cytotoxicity against the human cancer cell line HeLa and the embryonic lung MRC5 cell line. In a first screening at a concentration of 10 microg/ml, 92 extracts from 46 plants showed antiplasmodial activity (parasite growth inhibition >30%). The IC(50) values of the most active extracts were determined as well as their selectivity towards Plasmodium falciparum in comparison to their cytotoxic effects against the human cell lines. Six plants showed interesting antiplasmodial activity (IC(50) ranging from 0.4 to 8.6 microg/ml) with a good selectivity: two Menispermaceae, Arcangelisia flava (L.) Merr. and Fibraurea tinctoria Lour., and also Harrisonia perforata (Blanco) Merr. (Simaroubaceae), Irvingia malayana Oliv. ex Benn. (Irvingiaceae), Elaeocarpus kontumensis Gagn. (Elaeocarpaceae) and Anneslea fragrans Wall. (Theaceae).

Animals↗
PubMed2006

Laurentixanthones A and B, antimicrobial xanthones from Vismia laurentii.

A phytochemical investigation of the constituents of the roots of Vismia laurentii has resulted in the isolation of two xanthone derivatives named laurentixanthone A (1) (6-hydroxy-3,3-dimethyl-11-(3-methylbut-2-enyl)pyrano[2,3-c]xanthen-7(3H)-one) and laurentixanthone B (2) (1-hydroxy-5,6,7,8-tetramethoxyxanthone), along with 11 known compounds: 1,7-dihydroxyxanthone, vismiaquinone, vismiaquinone B, bivismiaquinone, 3-geranyloxy-6-methyl-1,8-dihydroxyanthraquinone, O(1)-demethyl-3',4'-deoxypsorospermin-3',4'-diol, 6-deoxyisojacareubin, 1,8-dihydroxy-6-methoxy-3-methylanthraquinone, kaempferol, friedelin and stigmasterol. The structures of compounds were established by means of spectroscopic methods. Furthermore, the compounds were screened for antimicrobial activities in vitro.

Anti-Infective Agents↗
PubMed2006

Minor biflavonoids from Lophira alata leaves.

The leaves of the Cameroonian medicinal plant Lophira alata afforded two new biflavonoids, lophirone L (1) and lophirone M (2), and the known luteolin and lithospermoside. Both biflavonoids were obtained in small quantities, and their structures show some new and unusual biflavonoid diversity.

Biflavonoids↗
PubMed2005

Isolation, characterization and antiplasmodial activity of steroidal alkaloids from Funtumia elastica (Preuss) Stapf.

Bioassay-guided fractionation of the EtOH extract of the stem bark of Funtumia elastica resulted in the isolation of four steroidal alkaloids, holarrhetine (1), conessine (2), holarrhesine (3) and isoconessimine (4). Their structures were determined on the basis of 1D- and 2D-NMR techniques and mass spectrometry. Compounds 1-4 exhibited in vitro antiplasmodial activity against the chloroquine-resistant strain FcB1 of Plasmodium falciparum with IC50 values ranging from 0.97 to 3.39 microM. They showed weak cytotoxicity against a rat cell line L-6 with IC50 values ranging from 5.13 to 36.55 microM.

Alkaloids↗
PubMed2005

In vitro antiplasmodial activity and cytotoxicity of 33 West African plants used for treatment of malaria.

Thirty-three plants commonly used in West tropical Africa by traditional healers for the treatment of malaria were collected and ethanolic extracts were obtained by decoction. The antiplasmodial activity of extracts was evaluated in vitro against the chloroquine-resistant FcB1 strain of Plasmodium falciparum. Cytotoxicity was determined on the human MRC-5 and the rat L-6 cell lines. Of the 33 plant extracts, eight (24.5%) showed significant antimalarial activity (IC(50) values ranging from 2.3 to 13.7 microg/ml), 14 (42.5%) weak activity (IC(50) values ranging from 15 to 50 microg/ml) and 11 (33%) appeared inactive (IC(50) values >50 microg/ml). Five plants were of particular interest, associating good antiplasmodial activity and weak cytotoxicity. These five included Nauclea latifolia with known antiplasmodial activity and four, Fagara macrophylla, Funtumia elastica, Phyllanthus muellerianus and Rauvolfia vomitoria, for which the description of antiplasmodial activity is entirely novel.

Animals↗
PubMed2005

Cherimolacyclopeptide D, a novel cycloheptapeptide from the seeds of Annona cherimola.

In a chemical investigation of the seeds of Annona cherimola, a natural cyclic heptapeptide, cherimolacyclopeptide D, were isolated and purified by HPLC with three known cyclic peptides, cherimolacyclopeptides A, B and C. The structure was established by various analyses including MS/MS fragmentation, spectroscopic and chemical evidences.

Annona↗
PubMed2005

Two cyclopeptides from the seeds of Annona cherimola.

Bioassay-guided fractionation of cytotoxic of methanol extract of the seeds of Annona cherimola provided two novel cyclic peptides, cherimolacyclopeptide E (1) and cherimolacyclopeptide F (2), which exhibited significant cytotoxic activity against the KB (human nasopharyngeal carcinoma) cell culture system. The peptide 1 and 2 were elucidated by MS/MS fragmentation experiments using a Q-TOF mass spectrometer equipped with an ESI source, extensive 2D NMR analyses and chemical degradation.

Amino Acid Sequence↗
PubMed2005

Antimicrobial biflavonoids from the aerial parts of Ouratea sulcata.

Investigation of the aerial parts of Ouratea sulcata led to the isolation of a biflavonoid named sulcatone A, together with the known compounds, 3-hydroxy-2,3-dihydroapigenyl-[I-4',O,II-3']-dihydrokaempferol, amentoflavone, lophirone A, agathisflavone, stigmasterol and stigmasteryl-3-O-beta-D-glucopyranoside. The structure of the compound was assigned as apigenyl-[I-4',O,II-3']-dihydrokaempferol, by means of spectroscopic analysis. Sulcatone A and 3-hydroxy-2,3-dihydroapigenyl-[I-4',O,II-3']-dihydrokaempferol exhibited significant in vitro antimicrobial activities against a range of microorganisms.

Anti-Bacterial Agents↗
PubMed2004

Allanxanthone B, a polyisoprenylated xanthone from the stem bark of Allanblackia monticola Staner L.C.

In addition to five known compounds including three xanthones, tovophyllin A, rubraxanthone and garciniafuran, one pentacyclic triterpene, lupeol and one phytosterol, stigmasterol, a polyisoprenylated xanthone named allanxanthone B was isolated from the stem bark of Allanblackia monticola. The structure of the new compound was assigned as 2-geranyl-1,3,6-trihydroxy-2',2'-dimethyl[5',6':7,8]xanthone by means of spectroscopic analysis. The antimicrobial activities of some of these compounds against a range of micro-organisms are also reported.

Anti-Bacterial Agents↗
PubMed2004

Antimicrobial and cytotoxic agents from Calophyllum inophyllum.

The study of the chemical constituents of the root bark and the nut of Calophyllum inophyllum has resulted in the isolation and characterization of a xanthone derivative, named inoxanthone, 3, together with 12 known compounds: caloxanthones A, 4 and B, 5, macluraxanthone, 6, 1,5-dihydroxyxanthone, 7, calophynic acid, 8, brasiliensic acid, 9 inophylloidic acid, 10, friedelan-3-one, 11, calaustralin, 12, calophyllolide, 13, inophyllums C, 14 and E, 15. Their structures were established on the basis of spectral evidence. Their in vitro cytotoxicity against the KB cell line and their antibacterial activity and potency against a wide range of micro organisms were evaluated.

Anti-Bacterial Agents↗
PubMed2004

New alkaloids from Daphniphyllum calycinum.

Four new alkaloids, 17-hydroxyhomodaphniphyllic acid (1), daphcalycinosidine C (2, a new iridoid alkaloid), yuzurimine E (3), and yuzurimic acid B (4), were isolated from the seeds of Daphniphyllum calycinum. The structures of these Daphniphyllum alkaloids were determined by spectroscopic analysis including mass spectrometry and 2D NMR.

Alkaloids↗
PubMed2004

A cytotoxic cyclic heptapeptide from the seeds of Annona cherimola.

From a methanol extract of the seeds of Annona cherimola, a new cyclic heptapeptide, cherimolacyclopeptide C, has been isolated. The structure was elucidated on the basis of the MS/MS fragmentation using a Q-TOF mass spectrometer equipped with an ESI source, extensive 2D NMR experiments, and chemical degradation. Cherimolacyclopeptide C exhibited significant in vitro cytotoxic activity against KB cells, with an IC(50) value of 0.072 microM.

Amino Acid Sequence↗
PubMed2003

Flavonoids from Andrographis lineata.

Three flavonoids, 5,7,2',3',4'-pentamethoxyflavone (1), 2'-hydroxy-2,4',6'-tri methoxychalcone (2) and dihydroskullcapflavone I (3), together with 17,19,20-trihydroxy-5beta, 8alpha H, 9beta H,10alpha-labd-13-en-16,15-olactone (4), a known diterpenoid and six known flavonoids, 5-hydroxy-7,8-dimethoxyflavanone (5), 5-hydroxy-7,8,2',3',4'-pentamethoxyflavone (6), 5,2'-dihydroxy-7-methoxyflavanone (7), 5,2'-dihydroxy-7,8-dimethoxyflavone (8), 5,2'-dihydroxy-7-methoxyflavone (9) and 5,2'-dihydroxy-7-methoxyflavone 2'-O-beta-D-glucopyranoside (10) were isolated from the whole plant of Andrographis lineata. The structures of these compounds were elucidated on the basis of spectral and chemical studies.

Andrographis↗
PubMed2003

A flavone and an unusual 23-carbon terpenoid from Andrographis paniculata.

Phytochemical investigation of the roots and aerial parts of Andrographis paniculata Nees yielded a new flavone, 5-hydroxy-7,2',6'-trimethoxyflavone and an unusual 23-carbon terpenoid, 14-deoxy-15-isopropylidene-11,12-didehydroandrographolide together with five known flavonoids and four known diterpenoids. The structures of these compounds were determined on the basis of spectral and chemical studies.

Alkenes↗
PubMed2003

Two biflavonoids from Ouratea flava stem bark.

In a chemical investigation on the stem bark of Ouratea flava, two biflavonoids: 1-[3-(2,4-dihydroxy-benzoyl)-4,5,6-trihydroxy-2-(4-hydroxy-phenyl)-benzofuran-7-yl] -3-(4-hydroxy-phenyl) -propenone (flavumone A) and 3-(2,4-dihydroxy-benzoyl)-4-hydroxy-2,7-bis-(4-hydroxy-phenyl) -7,8- dihydro-furo[2,3-f]chromen-9-on (flavumone B) were isolated along with five known flavonoids. Their structures were established by various analyses including 2D-NMR spectroscopy.

Flavonoids↗