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Biomedical subjects

Bin Li

Publications and source records attributed to Bin Li.

At least 91 records · Page 5Linked to original sources
PubMed2006

[Clinical application of sentinel lymph node detection to early stage cervical cancer].

BACKGROUND & OBJECTIVE: Sentinel lymph node (SLN) identification has been increasingly used in the treatment design of a variety of solid tumors, particularly breast cancer and melanoma. A negative SLN predicts the absence of tumor metastases in the regional lymph nodes with high accuracy. This study was to investigate the clinical value of combined isotope-dye technique for detecting SLN, and to evaluate the accuracy of SLN in predicting the pelvic lymph nodes status in patients with early stage cervical cancer. METHODS: A total of 27 patients with early stage cervical cancer,scheduled for radical hysterectomy and total pelvic lymphadenectomy, were eligible for the study. Lymphoscintigraphy was performed with injection of radioactivity isotope (99m)Tc-labeled dextran ((99m)Tc-DX) into the uterine cervix 16 h before surgery. Methylthioninium (4 ml) was injected into the same points as (99m)Tc-DX during surgery, and the patients underwent lymphatic mapping with a handheld gamma-detecting probe. After resection of SLNs, standard radical hysterectomy with pelvic lymph node dissection was performed. All removed lymph nodes, including the SLNs, were examined by routine histopathology. The histopathologic results of SLNs and non-SLNs were compared. RESULTS: The detection rates of SLN were 96.3% by blue dye method, and 100% by radiolabeled tracer or combined isotope-dye. Blue dye method identified 61 SLNs, radiolabeled tracer identified 69 SLNs, and combined isotope-dye identified 70 SLNs. Preoperative SPECT/CT fusion images detected 4 SLNs in the parametrium. Seven (25.9%) patients had lymph node metastasis. The sensitivity, accuracy, negative predictive value, and false negative rate of SLN detection were 85.7% (6/7), 96.3% (26/27), 95.2% (20/21), and 14.3% (1/7), respectively. CONCLUSIONS: SPECT/CT imaging not only is superior to planar imaging in detecting SLN but also enables precise localization of SLNs. The combined isotope-dye method can improve the accuracy of SLN detection. SLN detection can accurately predict the pelvic lymph nodes status in early stage cervical cancer.

Adult↗
PubMed2006

[Chemical constituents from root of Lasianthus acuminatissimus I].

OBJECTIVE: To investigate the chemical constituents from the root of Lasianthus acuminatissimus of Rubiaceae. METHOD: Normal, reverse phase silica gel, macroporous resin column and HPLC chromatography were used for isolation. Spectroscopic methods (13C-NMR, 1H-NMR, DEPT, EI-MS) were used for identification. RESULT: Six compounds were isolated and elucidated as isoscopletin(I), isoscopletin-6-O-beta-D-glucopyranoside (II), beta configuration of Mudanoside-A (Ill), asperuloside (IV), (-)-pinoresinol 4-O-beta-D-glucopyranoside (V), daucosterol (VI). CONCLUSION: These compounds were obtained from this plant for the first time.

Cyclopentane Monoterpenes↗
PubMed2006

[Gene regulatory mechanism of hairy and enhancer of split related-1 for angiogenesis factors].

OBJECTIVE: To study the roles of hairy and enhancer of split related-1 (HESR-1) in maintenance of the mature, quiescent vessel and angiogenesis. METHODS: Human umbilical vein endothelial cells (HUVEC) were cultured. The full-length coding sequence of HESR-1 was cloned into PcDNA3.1 + using standard protocols. HESR-1 specific siRNA was synthesized and cloned into the RNAi-Ready pSIREN-RetroQ ZsGreen Vector. The constructed PcDNA3.1 + HESR-1 plasmid were transfected into HUVEC for the overexpression of HESR-1, and HESR-1-RNAi plasmid were transfected into HUVEC to silence the HESR-1 gene, the expression of KDR, ALK-1 and Ang-1 in HUVEC were analyzed by RT-PCR and Western blot. RESULTS: The expression of KDR was down-regulated and ALK-1 and Ang-1 were up-regulated in HUVEC with the overexpression of HESR-1; The expression of KDR was up-regulated and ALK-1 and Ang-1 were down-regulated the HESR-1 in HUVEC by RNAi. CONCLUSION: HESR-1 may play an important role in maintenance of vessel in quiescent and control angiogenesis by the regulation of the expression of KDR, ALK-1 and Ang-1.

Activin Receptors, Type II↗
PubMed2006

[The AHP of criteria analysis weighting for assessment on medical equipment suppliers].

In order to improve the management of the supplier chain, it is necessary to setup a assessment system on suppliers. The assessment includes lot's of criteria such as quality, cost, service and delivery capabilities. The different criteria should have different weighting. The AHP method is chosen here in the paper to analyse the weighting of individual criteria.

Equipment and Supplies↗
PubMed2006

[Clinical anatomy measurement of accessory nerve in neck dissection].

OBJECTIVE: To investigate the relations between accessory nerve and its surrounding structures. METHODS: One hundred and thirty six patients were divided into two groups: has or has no neck surgical history. Neck dissection were performed and the four distance were measured simultaneously. The distance of accessory nerve and the great auricular nerve going out the posterior edge of sternocleidomastoid muscle; the distance of the point accessory nerve going out the posterior edge of sternocleidomastoid muscle to clavicular midpoint; the distance of the point accessory nerve going out the posterior edge of sternocleidomastoid muscle to sternoclavicular articulation; the distance of the point accessory nerve enter trapezius muscle to clavicular midpoint. RESULTS: In no neck dissection group, the point accessory nerve going out sternomastoid muscle were supra the point of great auricular nerve going out the sternomastoid muscle, the average length of two points is (0. 61 +/- 0. 35) cm , the significance has not observed between genders (P > 0.05), however, there has significant difference between two groups of has or has no neck surgical history (P < 0.05). 88.2% (112/127) accessory nerve going out supra the great auricular within 1.0 cm, 11.8% (15/127) within 1.0 approximately 2.0 cm. 67.7% (86/127) accessory nerve adopt branch from cervical plexus before entering trapezius. The distances of the point accessory nerve going out the posterior edge of sternocleidomastoid muscle to clavicular midpoint and to sternoclavicular articulation were significant relative not with before neck surgical history but gender. The distance of the point accessory nerve enter trapezius muscle to clavicular midpoint is (4.96 +/- 0.78) cm, it has no difference both before neck surgical history and gender (P > 0.05). CONCLUSION: In no neck surgical history group,both of the distance that accessory nerve and the great auricular nerve going out the posterior edge of sternocleidomastoid muscle and the point accessory nerve enter trapezius muscle to clavicular midpoint were helpful for search accessory nerve in surgery. But in patients who have neck surgical history or great auricular have been injured, accessory nerve could be looked for associating with the distances of the point accessory nerve going out the posterior edge of sternocleidomastoid muscle to clavicular midpoint and to sternoclavicular articulation; the distance of the point accessory nerve enter trapezius muscle to clavicular midpoint.

Accessory Nerve↗
PubMed2006

[Latest advances on the nomenclature for factors of the SLA class-I system].

A new nomenclature system for the SLA class-I system based on DNA sequencing and phylogenetic analysis has been published recently. The naming conventions of SLA class-I loci, allele groups, alleles and haplotypes are particular represented, and the requirements for naming a new allele are also recapitulatively described in this paper. Considering the nomenclature system of SLA class-I, it will has important significance towards human transplantation immunology, swine immunogenetics and breeding for pig disease resistance in the future.

Alleles↗
PubMed2006

Effects of fruit pre-harvest bagging on fruit quality of peach (Prunus persica Batsch cv. Hujingmilu).

Experiments were conducted to test the effects of pre-harvest bagging on fruit ripening and quality of peach (Prunus persica Batsch cv. Hujingmilu). Young fruits, at 50 days after full bloom (DAFB), were covered with bags made of single-, double-, and triple-layers of orange paper bag with 27.0%, 13.9% and 8.2% sunlight transmission, respectively. Ethylene production and respiration rate were measured, and fruit quality was analyzed at 111, 114, 117, 120 (firm-ripe stage) and 124 DAFB (full-ripe stage). Single- and triple-layer bagged fruits had higher ethylene production rates than double-layer bagged and un-bagged fruits. The skin of un-bagged fruit had higher brightness (L-value) but smaller hue angle (h degrees) at the full-ripe stage compared with that of bagged fruit. Flesh firmness of un-bagged fruit was higher than that of bagged fruit until the firm-ripe stage, although triple-layer bagged fruit had higher firmness than un-bagged fruit at the full-ripe stage. Total soluble solids in juice of single-layer bagged fruit were a little higher than those of other treated fruits at the full-ripe stage. Single-layer bagged fruit showed the highest level of gamma-decalactone, a main characteristic aroma of peach and total lactones at the firm-ripe and full-ripe stages. It was concluded that 'Hujingmilu' peach had high quality with abundant aromas when the fruits were bagged with single-layer orange paper bags at 50 DAFB. The biosynthesis of gamma-decalactone and other aromas may be affected by light to some extent.

Chromatography, Gas↗
PubMed2006

A new sesquiterpene lactone from the roots of Lasianthus acuminatissimus.

AIM: To study the active constituents for the treatment of rheumatoid arthritis from the ethyl acetate extracts of the roots of Lasianthus acuminatissimus Merr. METHODS: Various chromatographic techniques were used to separate and purify the constituents. Their structures were established on the basis of 1D, 2D NMR and HRMS spectroscopic analyses and their preliminary evaluation of anti-inflammation effect on the release of beta-glucuronidase was carried out. RESULTS: Eight compounds were isolated and identified as lasianthuslactone A (1), codonolactone (2), 2,5-dimethoxy-1, 4-benzoquinone (3), uncargenin A (4), nonadecyl alcohol (5), 13-docosenoic acid (6), tetracosanoic acid (7) and beta-sitosterol (8). Compound 3 showed a significant inhibitory effect on release of beta-glucuronidase rat polymorphous nuclear leukocytes activated by platelet activating factor (PAF). CONCLUSION: Compound 1 is a new one, the others were isolated from the plant for the first time and 3 is one of active anti-inflammation compound in the plant.

Animals↗
PubMed2006

[Expression of c-fos protein after muscle contusion in experimental rats].

OBJECTIVE: To study the relationship between the expression of c-Fos protooncogene and skeleton muscle contusion of rats, and to search for a sensitive marker of timing for skeleton muscle contusion. METHODS: Fifty Sprague-Dawley rats were randomly distributed into control group and experimental groups. The expression of c-fos was microscopically observed by immunohistochemical method. RESULTS: With the time prolonged the c-fos positive intensity and area were increased. Positive expression of c-fos protein appeared at 15 min after skeleton muscle contusion, and reached to the peak at 1h after skeleton muscle contusion, then decreased gradually and returned to the normal level on 1d after skeleton muscle contusion. CONCLUSION: The detection of the expression of c-fos protein could be a sensitive marker for timing skeleton muscle contusion.

Animals↗
PubMed2006

[To estimate early postmortem interval by investigating changes of rats' gastrocnemius excitability].

OBJECTIVE: To investigate the changes of excitability of rats' gastrocnemius after rats were died from mechanic asphyxia, so as to make an objective evidence for estimating of the early postmortem interval. METHODS: After rats were sacrificed by mechanic asphyxia, the reaction of rats' gastrocnemius to variable electric stimulation at different period after death were recorded by electricphysiological method. RESULTS: Changes in the properties of excitable tissue to electric stimulate in rats' gastrocnemius after mechanic asphyxia seems to be well correlated with the early postmortem interval and appeared a linear relation in certain period. CONCLUSION: The regular changes of excitability in muscle might be a useful means for estimating of early postmortem interval.

Action Potentials↗
PubMed2006

[A study on the expression of HSP70 in endothelial cells pretreated with ethanol and fluid shear stress].

OBJECTIVE: To detect whether ethanol can affect the expression of HSP70 in endothelial cells under fluid shear stress. METHODS: Ethanol at different concentrations was added to the culture medium of endothelial cells, EA. Hy926, which was treated statically or under 1Pa fluid shear stress. After the incubation of 1 h, 2 h, 4 h and 6 h, the expression of HSP70 was detected by immunohistochemical method(SP). RESULTS: In the control group, the expression of HSP70 was negative under static state, while it was positive under 1Pa fluid shear stress lasting 4 h even without ethanol. No statistic difference was found between the 50 mg/dL ethanol group and the control group with the same treatment time of fluid shear stress. HSP70 expression was found under static state with 150 mg/dL ethanol after 4 h or 300 mg/dL ethanol after 2 h respectively. The expression increased greatly under 1Pa fluid shear stress in the same ethanol concentrations. CONCLUSION: Medium to high ethanol concentration in coordination with fluid shear stress can strongly stimulates the expression of HSP70 by a kinetic mechanism of time-dependent.

Cells, Cultured↗
PubMed2006

[Applications of the digital network in radiotherapy].

Construction of the digital radiotherapy network is the need of contemporary radiotherapy, and is also an important part of the construction of a modernized hospital. This article analyzes the basic technical requirements and functions of the digital radiotherapy network, and gives opinions on the application of the digital radiotherapy network.

Computer Communication Networks↗
PubMed2006

[Effects of styrene on the dopaminergic transmitter content and monoamine oxidase activity in different sections of rat brain].

OBJECTIVE: To observe the effect on dopaminergic transmitter content and of monoamine oxidase (MAO) activity at dose of experiment in different sections of rat brain exposed bu acutely and subacutely styrene. METHODS: Rats were administrated orally with styrene of at dose of 600mg/kg for acute, 150, 300 and 600mg/kg for subacute experiment; recovery group were observed after 3 weeks exposure of styrene and intervened group were injected intraperitoneally at dose of 600mg/kg Levodopa (L-dopa) ; the urinary metabolites of styrene mandelic acid (MA) and phenylglyoxylic acid (PGA) were monitered as inner dosage, and the content of dopamine (DA) and activity of MAO were evaluated. RESULTS: The result indicated that the content of urinary MA and PGA were associated with dosage positively, and MA may be more sensitive as inner dosage of styrene exposure since the background of PGA. Levels of DA in retina, hypophysis and striatum were decreased after styrene exposure, the activities of MAO in hypophysis were increased and were reduced in retina and striatum. CONCLUSION: It was suggested dopaminergic system could be participated in styrene neurotoxicity.

Animals↗
PubMed2006

[Protective effect of reduced glutathione on cytotoxicity induced by hexavalent chromium [Cr(VI)] in L-02 hepatocyte].

OBJECTIVE: To explore the effect of reduced glutathione (GSH) on cytotoxicity induced by hexavalent chromium (Cr(VI)) in L-02 hepatocyte. METHODS: The test has three groups:the groups of (Cr(VI), the groups of GSH, the groups of Cr (VI) and GSH. The survival rate of L-02 hepatocyte is assessed on the reductions of tetrazolium dye (MTT). RESULTS: Significant cytotoxicities of L-02 hepatocyte were observed at the concenations of 2,4,8,16,32 and 64 micromol/L Cr (VI). Concentration-dependent decrease in cell survival rate of Cr (VI)-treated L-02 hepatocytes were observed (r = -0.910) Protective effect on all concentrations of Cr(VI) (2 - 64 micromol/L) at the dose of 20 micromol/LGSH were found. CONCLUSION: The results demonstrated that proper concentrations of GSH could have protective effect on cytoxicity induced by Cr(VI) in L-02 hepatocyte. GSH of too low or too high concentrations don't has this effect.

Cell Line↗
PubMed2006

[Study of L-02 hepatocyte apoptosis induced by hexavalent chromium associated with mitochondria function damage].

OBJECTIVE: To explore the effect of hexavalent chromium on apoptosis of L-02 hepatocytes and the functions of mitochondria. METHODS L-02 hepatocytes in all tests were incubated with 0,2,4,8,16,32 [see text] 64 micromol/L of Cr(VI) for 6h. Apoptosis of L-02 hepatocytes in the presence of Cr(VI) was quantified by flow cytometry (FCM). The permeability transition pore (FTP) of mitochondria and mitochondrial membrane potential as indicators of mitochondrial damage were measured by fluorescent spectrometer. RESULTS: Concentration-dependent decrease in cell apoptosis rate of Cr(VI)-treated L-02 hepatocytes were observed. The results of permeability transition pore (PTP) of mitochondria, mitochondrial membrane potential in all concentrations of Cr(VI) had significant difference when compared to the control cells (P < 0.05). CONCLUSION: The results demonstrated that L-02 hepatocytes apoptosis induced by Cr(VI) associated with mitochondrial damages.

Apoptosis↗