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Bing-ya Liu

Publications and source records attributed to Bing-ya Liu.

25 records · Page 2Linked to original sources
PubMed2005

[COX- 2 expression in gastric cancer and its relationship with lymphangiogenesis and lymph node metastasis].

OBJECTIVE: To investigate the expression of cyclooxygenase-2 (COX-2) and vascular endothelial growth factor-C (VEGF-C) in human gastric cancer, the relationship between their expression and the clinicopathological features,as well as the relationship between these two parameter expression and lymphangiogenesis and lymph node metastasis. METHODS: COX-2 and VEGF-C expressions were detected in 63 gastric cancer samples by immunostaining. Lymphangiogenesis was evaluated by immunostaining with the specific antibody LYVE-1. RESULTS: The expression rates of COX-2 and VEGF-C were 66.7% (42/63), 52.4% (33/63), respectively in 63 gastric cancer specimens. LYVE-1 was positive in 35 cases (35/63), which indicated lymphangiogenesis in the tumors. The expression of COX-2 was significantly correlated with the expression of VEGF-C, tumor lymphangiogenesis and lymphatic metastasis (P< 0.05), however not gender, tumor size, tumor location, Lauren classification and serosa invasion (P< 0.05). CONCLUSIONS: In gastric cancer, the expression of COX-2 is significantly associated with VEGF-C expression, lymphangiogenesis and lymphatic metastasis. COX-2 may up-regulate the expression of VEGF-C, which induces lymphangiogenesis and accordingly contributes to lymphatic metastasis.

Aged↗
PubMed2005

[Expression and clinical significance of cancer-related gene MPS-1 in gastric cancer].

OBJECTIVE: To investigate the expression of cancer-related gene MPS-1 in gastric cancer and to evaluate its significance in clinical diagnosis and therapy. METHODS: The mRNA expression of MPS-1 was determined by polymerase chain reaction after reverse transcription (RT-PCR) in cancer tissues and adjacent non-cancerous tissues from 42 cases with gastric cancer. The expression levels of MPS-1 in 6 gastric cancer cell lines (AGS, MKN-45, SGC 7901, KATO III, N-87 and SNU-1) were also determined by RT-PCR and Western blot. RESULTS: The MPS-1 mRNA was expressed in all tissues and cell lines. The mRNA expression level of MPS-1 in cancer tissues were 1.37+/- 0.87, significantly higher than 0.99+/- 0.67 in adjacent normal gastric mucous tissues (P< 0.01). The expression of MPS-1 was correlated with TNM stage (P< 0.05), but not with age, gender, tumor size and differentiation. The expression level of MPS-1 mRNA in the primary lesions was hig her in the patients with TNM stages III, IV than those with TNM stages I, II. Meanwhile, RT-PCR and Western blot showed the same results that MPS-1 expression was higher in the six gastric cancer cell lines as compared with that in the normal gastric cell line GES-1. CONCLUSION: The high expression of MPS-1 in gastric cancer indicates that MPS-1 might play an important role in gastric carcinogenesis,which may provide a new target in immunotherapy for gastric cancer.

Cell Cycle Proteins↗
PubMed2005

[Transcription factor Sp1 expression in gastric cancer and its prognostic value].

OBJECTIVE: To investigate the expression of transcription factor Sp1 in human gastric cancer tissues and normal gastric mucosa, and its prognostic significance. METHODS: By using immunohistochemistry, we studied the Sp1 expression patterns in 65 cases of gastric cancer with various clinico-pathologic characteristics, and 40 normal gastric mucosa specimens obtained from patients who underwent partial gastrectomy for benign gastric diseases. The significance of Sp1 expression on the survival of patients was evaluated. RESULTS: The expression rate of Sp1 in normal gastric mucosa was 12.5% (5/40). The positively stained glandular cells were mainly limited to those in the neck region. Cells at the basal portion of the gland were essentially negative. In sharp contrast, Sp1 expression rate in gastric cancer lesions was 53.8% (35/65). The medium survival time in patients who had a tumor with negative, weak and strong Sp1 expression was 1700, 1560 and 1026 days, respectively (P = 0.036). Sp1 protein expression was closely related to the depth of tumor invasion and TNM stage (P = 0.001, P = 0.026), but not related to the number of metastatic lymph nodes and Lauren's classification (P = 0.306, P = 0.667). CONCLUSION: Normal and malignant gastric tissues have unique Sp1 expression patterns. Sp1 might be served as an independent prognostic factor.

Adenocarcinoma↗
PubMed2004

[Expression of MAGE-1 and MAGE-3 genes in gastric cancer and gastric biopsy tissues and its clinical significance].

AIM: To detect the expression of melanoma antigen-1 and -3 genes (MAGE-1 and MAGE-3) and explore its significance in immunotherapeutic application. METHODS: The expression of MAGE-1 and MAGE-3 in gastric cancer, para-tumorous normal mucosa, and gastric biopsy tissues were detected by RT-PCR. The target genes in RT-PCR product were verified by means of DNA sequencing. RESULTS: MAGE-1 expression was positive in 26 out of 36 (72.22%) cases of gastric cancer, MAGE-3 positive in 25 out of 36(69.44%), and both MAGE-1 and MAGE-3 positive in 19 out of 36 (52.78%). Positive expression rates of MAGE-1, MAGE-3 and both MAGE-1 and MAGE-3 were 47.22%, 44.44% and 30.55%, respectively in para-tumorous normal mucosa, while 29.73%, 27.03% and 8.1% in gastric biopsy tissues The positive rates of MAGE-1 and MAGE-3 gene expressions in tumor tissues was significant higher than those its parallelism mucosae and biopsy tissues from chronic gastritis. The expressions of MAGE-1 and MAGE-3 genes in tumor tissues were relevant to intruded depth and the differentiated degree of tumor cells (P<0.05). CONCLUSION: MAGE-1 and MAGE-3 protein may be used as targets molecules of immunotherapy for gastric cancer and indexes for follow-up study of chronic gastritis as well.

Antigens, Neoplasm↗
PubMed2003

[A preliminary study of endoscopic ultrasonography in the preoperative staging of early gastric carcinoma].

OBJECTIVE: To study the clinical value of endoscopic ultrasonography (EUS) in the preoperative staging of early gastric carcinoma. METHODS: EUS was performed in 149 gastric carcinoma patients proved by biopsy (including 33 patients with early gastric cancer), of which the results were compared with postoperative pathologic findings. RESULTS: The accuracy of EUS in determining the T stage of gastric carcinoma was 80.3% (T1 81.8%, T2 70.4%, T3 88.9%, T4 71.4%). The accuracy of EUS in differentiating early gastric carcinoma from advanced ones was 95.1%, and the accuracy of EUS in differentiating mucosal cancer from submucosal cancer was only 63.6%. The diagnostic accuracy of EUS for mucosal and submucosal cancer was 52.9% and 75%, with positive predictive value of 90% and 70.6%, respectively. The accuracy of invasion depth of EUS for the bulging and flat type of early gastric carcinoma was 100%, whereas the accuracy was only 58.6% for the depressed type. The accuracy of invasion depth of the differentiated and undifferentiated early cancer was 71.4%and 57.9%, without any significant difference (P > 0.05). The accuracy of invasion depth of EUS for early gastric carcinoma decreased as tumor size increased. The diagnostic accuracy of lymph node status of early gastric carcinoma by EUS was 90.9%, and the sensitivity and specificity of lymph node metastasis was 66.7% and 96.3%, respectively. CONCLUSION: The clinical value of endoscopic ultrasonography in the preoperative staging of early gastric carcinoma is relatively high.

Adenocarcinoma↗
PubMed2002

[In situ gene therapy for murine gastric carcinoma with UPRT/5-FU enzyme/prodrug system mediated by retrovirus].

BACKGROUND & OBJECTIVE: 5-Fluorouracil(5-FU), a widely used chemotherapeutic drug, has a limited overall effect in the treatment of human solid tumors due to resistance. This study was designed to investigate if antitumor activation of 5-FU could be enhanced by transfection of uracil phosphoribosyltransferase(UPRT) gene. METHODS: The UPRT gene encoding uracil phosphoribosyltransferase was amplified from Escherichia Coli K12 genome and subcloned into retrovirus expression vector pLXSN, Recombinant retrovirus was packaged and used further to infect murine gastric cancer cell line MFC. The sensitivity of MFC transfected with UPRT gene to 5-FU was determined by MTT method. In situ gene therapy was performed by regional repeated injections of concentrated and purified recombinant retrovirus carrying UPRT gene intratumorally and followed by administration of 5-FU intraperitoneally(i.p.). RESULTS: The 5-FU sensitivity in MFC transfected with the UPRT gene increased 17.26-fold compared to the control cells. In situ transfection of the UPRT gene mediated by retrovirus vector followed by the administration of 5-FU (10 mg/kg) significantly inhibited the tumor growth (P < 0.005) with an inhibition rate of 87.18% and prolonged the survival. CONCLUSION: Transfection of UPRT gene can render the murine gastric cancer cell line MFC be more sensitive to low concentration of 5-FU and significantly improve the antitumor effect of 5-FU both in vitro and in vivo.

Animals↗