PubMed Health⌕ Search

Biomedical subjects

Bo Wen

Publications and source records attributed to Bo Wen.

27 records · Page 2Linked to original sources
PubMed2004

Optimization of reversed-phase microcapillary liquid chromatography for quantitative proteomics.

Currently, the field of shotgun proteomics relies primarily on the separation of peptides by reversed-phase microcapillary chromatography (RP-microLC) combined with either electrospray ionization (ESI) or matrix-assisted laser desorption ionization (MALDI) and tandem mass spectrometry (MS/MS) for protein identification as well as quantification. For this purpose we herein describe construction of a RP-microLC-ESI column-emitter along with optimized microLC conditions for using the device to quantify pair-wise changes in protein expression via the isotope coded affinity tag (ICAT) method that also maximize peak capacity. These optimized RP-microLC parameters required a balance be reached between the disparate needs of quantification which requires good peak shape and identification (i.e. proteome coverage) of proteins via peptide collision induced dissociation (CID) which requires peak capacity be maximized. A complex biological sample from a study of murine acetaminophen toxicity in hepatocyes was chosen for method development because of the high level complexity, but the biological results are not the focus of this manuscript.

Amino Acid Sequence↗
PubMed2004

Analyses of genetic structure of Tibeto-Burman populations reveals sex-biased admixture in southern Tibeto-Burmans.

An unequal contribution of male and female lineages from parental populations to admixed ones is not uncommon in the American continents, as a consequence of directional gene flow from European men into African and Hispanic Americans in the past several centuries. However, little is known about sex-biased admixture in East Asia, where substantial migrations are recorded. Tibeto-Burman (TB) populations were historically derived from ancient tribes of northwestern China and subsequently moved to the south, where they admixed with the southern natives during the past 2600 years. They are currently extensively distributed in China and Southeast Asia. In this study, we analyze the variations of 965 Y chromosomes and 754 mtDNAs in >20 TB populations from China. By examining the haplotype group distributions of Y-chromosome and mtDNA markers and their principal components, we show that the genetic structure of the extant southern Tibeto-Burman (STB) populations were primarily formed by two parental groups: northern immigrants and native southerners. Furthermore, the admixture has a bias between male and female lineages, with a stronger influence of northern immigrants on the male lineages (approximately 62%) and with the southern natives contributing more extensively to the female lineages (approximately 56%) in the extant STBs. This is the first genetic evidence revealing sex-biased admixture in STB populations, which has genetic, historical, and anthropological implications.

Chromosomes, Human, Y↗
PubMed2004

[Genetic structure of Tujia as revealed by Y chromosomes].

Tujia people call themselves "Bizika", which means aboriginal. Genetic study of Tujia is virtually absent. To characterize the genetic structure of Tujia,the distribution of 14 Y haplogroups was studied in Tujia populations sampled from Enshi, Hubei (31 males) and Jishou, Hunan (68 males). A total of eight haplogroups were observed in the Enshi and Jishou populations. The haplogroup frequencies of Tujia were compared with the frequencies of other related ethnic groups, including Northern Han, Southern Han, Tibetan-Burman speaking populations,Daic and Hmong-Mien. The principal component (PC) analysis was conducted and the PCs were plotted to explore the historical migrations. In addition, partial correlation analysis was performed to study the relationship between the first three PCs and the haplogroups. The PC2 revealed a cluster of Tujia groups including Longshan,Yongshun, and Enshi with Lahu, suggesting possible interaction between Tujia and the Di-Qiang groups. However,a similarity between Han and Tujia populations, though differentiated, were also observed. We postulated, by incorporating the results of archaeological and historical evidences, that the Ba people, the ancestors of the Tujia,might be related with Di-Qiang groups and inhabited the Tujia area initially before a substantial interaction with Han and other ethnic groups.

Asian People↗
PubMed2004

[Cytocompatibility of nanophase hydroxyapatite ceramics].

OBJECTIVE: To evaluate the cytocompatibility of nanophase hydroxyapatite ceramics in vitro. METHODS: Hydroxyapatite (HA) was prepared via wet method. The grain size of the hydroxyapatite in the study was determined by scanning electron microscope and atomic force microscope with image analysis software. Primary osteoblast culture was established from rat calvaria. Cell adherence and proliferation on nanophase hydroxyapatite ceramics and conventional hydroxyapatite ceramics were examined at 1, 3, 5, 7 days. Morphology of the cells was observed by microscope. RESULTS: The average grain size of the nanophase and conventional HA was 55 nm and 780 nm, respectively. Throughout 7 days period, osteoblast proliferation on the HA was similar to that on tissue culture borosilicate glass controls, osteoblasts could attach, spread and proliferate on HA. However, compared to conventional ceramics, osteoblast proliferation on nanophase HA was significantly better after 1, 3, 5 and 7 days. CONCLUSION: Cytocompatibility of nanophase HA was significantly better than conventional ceramics.

Animals↗
PubMed2004

[Determination of scutellarin and rutin in Shenfukang capsules by reversed-phase high performance liquid chromatography].

A rapid and simple method for the determination of scutellarin and rutin in Shenfukang capsules has been developed using reversed-phase high performance liquid chromatography. Scutellarin and rutin were first extracted by ultrasonic extraction with methanol-water (1:1, v/v). The resulting extracts were separated on a C18 column (4.6 mm i.d. x 150 mm, 5 microm). The mobile phase consisting of 0.02 mol/L KH2PO4 solution containing 1.0% glacial acetic acid-methanol (63:37, v/v) was run at a flow rate of 1.0 mL/min. The detection wavelength was 330 nm and the injection volume was 20 microL. Good linearity in the range of 10 mg/L to 300 mg/L was obtained for scutellarin and rutin. The method was successfully used for the quantification of scutellarin and rutin of Shenfukang capsules.

Apigenin↗
PubMed2003

[Origin of Hakka and Hakkanese: a genetics analysis].

Hakka is a distinctive Han Chinese population in Southern China speaking Hakkanese. The origin of Hakka has been controversial. In this report, we analyzed Y chromosomal markers in 148 Hakka males. Principle component analysis of Y-SNP haplotype distribution shows Hakka is clusteed strongly with the Han in Northern China, and is also close to She, a Hmong-Mien-speaking population, while the general Southern Han is fairly close to Daic populations. Admixture analysis revealed that the relative genetic contribution 80.2% (Han), 13% (She) and 6.8% (Kam) in Hakka. The network of Y-STR haplotype of M7 individuals in all concerned populations suggested two possible origins of Hmong-Mien contribution in Hakka: One is from Hubei and the other is from Canton. The Kam contribution in Hakka is likely from Kan-Yue, the ancient aborigine of Kiangsi (Jiangxi). The frequency of 9bp-deletion in Region V of mitochondrial DNA of Hakka is 19.7%, which is quite close to She but far from Han. We therefore concluded that genetically the majority of Hakka gene pool shall come from North Han with She contributing the most among all non-Han groups. Regarding the Hmong-Mien character of Hakkanese, the genetic structure of Hakka shows their core may be Kim-man, the ancient Hmong-Mien. We hypothesized that a great number of Han people from North China join this population in succession. Southern Chinese dialects, such as Hakkanese may also be those languages of Southern aborigines at first, and turn to extant appearance under the continuance effect of Northern Chinese.

China↗
PubMed2003

[The distribution of Y-chromosome haplotypes of Shui ethnic in Sandu,Guizhou].

Non-recombination region of Y-chromosome is a useful marker in tracing evolutionary history of paternal lineage. In the present study, total 92 individuals from Shui ethnic group in Sandu Shui Ethnic Group Autonomous County of Guizhou Province were inspected with 11 SNP sites including M7, M9, M15, M45, M89, M95, M119,M122, M130, M134 and YAP on Y-chromosome.All the subjects were required to be unrelated and without intermarriage with other ethnic groups within three generations. The haplotypes were analyzed by PCR-RFLP method. Four haplotypes H5,H8,H9 and H11 were detected with frequencies of 0.054, 0.044, 0.315 and 0.587, respectively.Principle component indicated that the paternal lineage of Shui ethnic group is much closer to Li ethnic group of Hainan Province and Bouyei ethnic group of Guizhou Province,which belong to the group of Zhuang-Dong branch of Sino-Tibetan language family. In addition genetic study of Shui coincides with its linguistic distribution.

English Abstract↗
PubMed2002

[Origins and migrations of Bouyei people in China--insights from Y chromosome and mitochondrion].

The frequency of Y-chromosome haplotypes consisted of thirteen single nucleotide polymorphism alleles in Bouyei people was investigated using PCR-RFLP. And the polymorphism of mitochondrial DNA Region V was studied. Nine Y-chromosome haplotypes (H1, H4, H5, H6, H7, H8, H9, H11, H12) and two mtDNA alleles (9-bp deletion) were obtained in the samples. The frequency distribution of these haplotypes and mtDNA polymorphisms in Bouyei people were very similar to that in Daic speaking populations including Zhuang, Li, Dong, and Yao people from Jinxiu, indicating a close genetic relationship among those populations implicating a common ancestry. A hypothesis of the origin of Bouyei people was proposed.

China↗