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Biomedical subjects

Bo Yu

Publications and source records attributed to Bo Yu.

79 records · Page 5Linked to original sources
PubMed2002

[Study on the factors in the complications of endonasal sinus surgery].

OBJECTIVE: To investigate the cause of complications occurring in the operations of endonasal sinus surgery(ESS) and how to deal with. METHOD: 526 patients performed with ESS were analyzed. 31 patients had complications. The incidence of the complications in patients operative history was 38.9%, the incidence of bleeding in which the bleeding amount was over 200 ml was 30.2%, the incidence in patients whose medical history was less than 10 years was 2.8%. RESULT: Except for one patient whose eyesight decreased without recovery, the rest recovered. CONCLUSION: The cause of the complications in ESS was related with the destroy of normal structure, sinus bony anatomic variations, amount of intraoperative bleeding and the time of medical history.

Adolescent↗
PubMed2002

[Variations of cellular membrane phospholipids with genesis and hepatic metastasis of large intestine cancer].

OBJECTIVE: To separate and detect membrane phospholipids and study the relationship of metabolism and signal transduction pathways of membrane phospholipids with genesis and hepatic metastasis of large intestinal carcinoma. METHODS: Forty-eight cases of colorectal cancer were detected with high performance liquid chromatography. Membrane phospholipids of phosphatidylinosital (PI), phosphatidylserine (PS), phosphatidylethanolamine (PE) and phosphatidylcholine (PC) in primary foci, paratumor intestinal mucosa and hepatic metastasis of large intestine cancer were separated and analyzed. RESULTS: In primary foci, paratumor intestinal mucosa, and hepatic metastasis of the 48 cases, the contents (mg/g) of PI were: 0.92 +/- 0.12, 1.57 +/- 0.14, 1.54 +/- 0.15 respectively, and PC 56.47 +/- 5.33, 108.57 +/- 6.37, 116.35 +/- 6.85. The contents of PI and PC were higher in primary foci and hepatic metastasis than in paratumor mucosa (F = 363.10, 870.10, P < 0.01). The contents of PE in the three tissues were 18.23 +/- 3.56, 42.02 +/- 4.33, 79.51 +/- 5.52, and in hepatic metastasis was the highest (F = 1 149.63, P < 0.01). PI and PC in primary foci of hepatic metastatic group and nonmetastasis group were not significantly different (t = 3.55, P > 0.05). But the PE content was higher in hepatic metastasis than in primary foci (t = 115.87, P < 0.01). CONCLUSIONS: Membrane phospholipids have obvious variations in genesis and hepatic metastasis of large intestine cancer. Rises of PI and PC were associated with genesis of large intestine carcinoma. The increase of PE content is closely related to invasion and hepatic metastasis of large intestine cancer.

Adult↗
PubMed2002

[Enhancement of ionizing radiation on liposome-mediated gene delivery in human rectal cancer HR-8348 cells].

OBJECTIVES: To investigate the effect of ionizing radiation on liposome-mediated gene delivery and find out a way to improve gene transfection. METHODS: Prior to liposome transfection, HR-8348 cells were irradiated at doses of 0, 2, 4, 8 Gy selected according to the surviving fraction line of HR-8348 cells after different dosage of radiation. After 36 h of liposome transfection, green fluorocytes were counted. The transfection efficiency was figured out and compared with each other. RESULTS: The transfection efficiency of liposome-mediated gene delivery was 21.32%, 62.17%, 68.00%, 77.78% at the dose of 0, 2, 4, 8 Gy respectively and the clinical dose (2 Gy) was as high as 62.17%. Combined radiation and liposome-mediated gene delivery achieved the approximate transfection efficiency of virus vector. CONCLUSION: Ionizing radiation can improve the transfection efficiency of liposome-mediated gene delivery markedly and it is expected to treat human malignancy with liposome-mediated gene delivery combined with radiation.

Dose-Response Relationship, Radiation↗
PubMed2002

[Double promoters induct suicide gene target killing of 5-FU drug-fast cancer cells].

OBJECTIVE: To study target killing of 5-FU drug-fast cancer cells with thymidylate synthase (TS) and p16 gene promoters inducting TK gene expression. METHODS: TS promoter was inserted to 5' end and p16 promoter inserted to 3' end of TK cDNA sequence, constructing recombinant plasmid of pXJ41. Human rectal cancer cell lines of HR-8348 and normal peripheral blood mononuclear cells (PBMC) were transfected with the recombinant plasmid. Plating efficiency was counted and survival rates of cells were tested with MTT method. And suppression rates of xenograft tumors in nude mice were examined. RESULTS: Recombinant pXJ41 with double promotors and TK gene was transfected into HR-8348, and positive expression of TS and TK was observed. The expression of TK gene was consistent with TS expression. Plating efficiency was 9/300, 92/300 in transfected HR-8348 and contrast cells respectively (t = 33.885, P < 0.01). Cancer cell growth rate reduced markedly in the transfected group. The suppression rate of xenograft tumor growth was 74.5%. With the recombinant pXJ41 to transfect PBMC, p16 expression was positive, but TK and TS expressions were negative. No damnification was observed in PBMC. CONCLUSIONS: TS and p16 double promoters are capable of inducting TK target killing of 5-FU drug-fast cancer cells, thus protecting normal cells and improving safety of gene therapy.

Animals↗
PubMed2002

[Estrogenicity of trans-resveratrol in immature mice in vivo].

To investigate the estrogenicity of trans-resveratrol in vivo, different doses of trans-resveratrol were administered orally (ig.) or subcutaneously (s.c.) to the weanling mice for 4 d. The results showed that 2.0 mg/kg.b.w of trans-transveratrol (ig or s.c.) could shorten vaginal opening latency periods (P < 0.05) and enhance keratinization of vaginal epithelium dramatically, increase uterine wet weights and uterine-body weight ratios significantly (P < 0.05, P < 0.01). Additionally, trans-transveratrol could thicken the columnar epithelial cells or increase the numbers of glands in uterine in immature mice. It was concluded that trans-transveratrol could appear estrogenic actions in vivo. Moreover, its activity via subcutaneous administration was higher than that via oral administration.

Animals↗
PubMedDate not supplied

Therapeutic effects of tumor reactive CD4+ cells generated from tumor-primed lymph nodes using anti-CD3/anti-CD28 monoclonal antibodies.

T-cell activation involves multiple signaling pathways. In this report, we conducted in vitro and in vivo immune function analysis of tumor-draining lymph node (TDLN) cells after anti-CD3/anti-CD28 activation versus anti-CD3 activation alone in a murine tumor model. In cytokine release assays, the doubly activated TDLN cells secreted significantly greater amounts of IFN-gamma and GM-CSF in response to specific tumor antigen compared with anti-CD3 activated cells. In adoptive immunotherapy, the doubly activated TDLN cells were more effective in mediating regression of 3-day pulmonary metastases compared with anti-CD3 activated cells. Although there was predominant proliferation of CD8+ cells after either activation procedure, the mean-fold expansion of CD4+ cells was significantly greater after anti-CD3/anti-CD28 activation than anti-CD3 activation alone. Using magnetic bead-enriched T-cell subsets, we found that either CD4+ or CD8+ doubly activated TDLN cells could independently mediate tumor regression. Furthermore, the doubly activated CD4+ cells were more effective than CD8+ cells in adoptive immunotherapy on a per-cell basis. The antitumor activity mediated by CD4+ or CD8+ cells could be significantly enhanced with the exogenous administration of IL-2. CD28 co-stimulation of tumor-primed lymphoid cells promotes the generation of potent tumor reactive effector cells, particularly CD4+ T cells, with antitumor activity in adoptive immunotherapy.

Animals↗