Biomedical subjects

Bodo M H Lange

Publications and source records attributed to Bodo M H Lange.

4 recordsLinked to original sources

PubMed2006

A centrosome-independent role for gamma-TuRC proteins in the spindle assembly checkpoint.

The spindle assembly checkpoint guards the fidelity of chromosome segregation. It requires the close cooperation of cell cycle regulatory proteins and cytoskeletal elements to sense spindle integrity. The role of the centrosome, the organizing center of the microtubule cytoskeleton, in the spindle checkpoint is unclear. We found that the molecular requirements for a functional spindle checkpoint included components of the large gamma-tubulin ring complex (gamma-TuRC). However, their localization at the centrosome and centrosome integrity were not essential for this function. Thus, the spindle checkpoint can be activated at the level of microtubule nucleation.

Animals↗

PubMed2005

Miniaturization in functional genomics and proteomics.

Proteins are the key components of the cellular machinery responsible for processing changes that are ordered by genomic information. Analysis of most human proteins and nucleic acids is important in order to decode the complex networks that are likely to underlie many common diseases. Significant improvements in current technology are also required to dissect the regulatory processes in high-throughtput and with low cost. Miniaturization of biological assays is an important prerequisite to achieve these goals in the near future.

Biological Assay↗

PubMed2002

Cdc37 is essential for chromosome segregation and cytokinesis in higher eukaryotes.

Cdc37 has been shown to be required for the activity and stability of protein kinases that regulate different stages of cell cycle progression. However, little is known so far regarding interactions of Cdc37 with kinases that play a role in cell division. Here we show that the loss of function of Cdc37 in Drosophila leads to defects in mitosis and male meiosis, and that these phenotypes closely resemble those brought about by the inactivation of Aurora B. We provide evidence that Aurora B interacts with and requires the Cdc37/Hsp90 complex for its stability. We conclude that the Cdc37/Hsp90 complex modulates the function of Aurora B and that most of the phenotypes brought about by the loss of Cdc37 function can be explained by the inactivation of this kinase. These observations substantiate the role of Cdc37 as an upstream regulatory element of key cell cycle kinases.

Animals↗

PubMed2002

Integration of the centrosome in cell cycle control, stress response and signal transduction pathways.

The identification of cell cycle control and signal transduction components on the centrosome has fostered the idea that the centrosome is more than a microtubule-organizing center. Indeed, recent molecular evidence suggests that the centrosome plays an active role not only in the regulation of microtubule nucleation activity, but also in the coordination of centrosome duplication with cell cycle progression, in stress response and in cell cycle checkpoint control. To achieve these roles, it interacts with a multitude of signal transduction molecules. The specificity of the interactions is mediated through anchoring proteins that bring centrosomal components and regulatory proteins into close proximity. The molecular composition and organization of the centrosome thus reflects its multiple functions.

Animals↗