Biomedical subjects

Bong Kyun Oh

Publications and source records attributed to Bong Kyun Oh.

6 recordsLinked to original sources

PubMed2005

Planar nitric oxide (NO)-selective ultramicroelectrode sensor for measuring localized NO surface concentrations at xerogel microarrays.

A planar ultramicroelectrode nitric oxide (NO) sensor was fabricated to measure the local NO surface concentrations from NO-releasing microarrays of varying geometries. The sensor consisted of platinized Pt (25 microm) working electrode and a silver paint reference electrode coated with a thin silicone rubber gas permeable membrane. An internal hydrogel layer separated the Pt working electrode and gas permeable membrane. The total diameter of the sensor was <or=50 microm, and demonstrated negligible analyte trapping effects. The sensitivity and response time of the ultramicroelectrode sensor to NO were 0.19+/- 0.07 pA nM(-1) and 1-4 s, respectively, with a 5 nM limit of detection. The sensor was employed to correlate the steady-state NO surface concentration and observed platelet adhesion resistance. Results indicate that the required steady-state NO concentration necessary to inhibit platelet adhesion to the micro-patterned xerogels depends on the xerogel geometry.

Animals↗

PubMed2005

Miniaturized glucose biosensor modified with a nitric oxide-releasing xerogel microarray.

An enzyme-based glucose biosensor modified to release nitric oxide (NO) via a xerogel microarray is reported. The biosensor design is as follows: (1) glucose oxidase (GOx) is immobilized in a methyltrimethoxysilane (MTMOS) xerogel layer; (2) a blended polyurethane/hydrophilic polyurethane coating prevents enzyme leaching and imparts selectivity for glucose; and (3) micropatterned xerogel lines (5 microm wide) separated by distances of 5 or 20 microm provide NO-release capability. This configuration allows for increased glucose sensitivity relative to sensors modified with NO-releasing xerogel films since significant portions of the sensor surface remain unmodified. Glucose diffusion to the GOx layer is thus less inhibited. The micropatterned NO-releasing biosensors generate sufficient NO levels to reduce both Pseudomonas aeruginosa and platelet adhesion without significantly compromising the enzymatic activity of GOx. The glucose response, linearity and stability of the NO-releasing micropatterned sensors are reported.

Absorption↗

PubMed2005

Direct detection of S-nitrosothiols using planar amperometric nitric oxide sensor modified with polymeric films containing catalytic copper species.

The direct amperometric detection of S-nitrosothiol species (RSNOs) is realized by modifying a previously reported amperometric nitric oxide gas sensor with thin hydrophilic polyurethane films containing catalytic Cu(II)/(I) sites. Catalytic Cu(II)/(I)-mediated decomposition of S-nitrosothiols generates NO(g) in the thin polymeric film at the distal tip of the NO sensor. Three different species are examined to create the catalytic layer: (1) a lipophilic Cu(II)-ligand complex; (2) Cu(II)-phosphate salt; and (3) small (3-microm) metallic Cu(0) particles. All three catalytic layers yield reversible amperometric response in proportion to the concentration of S-nitrosothiols (e.g., nitrosocysteine, nitrosoglutathione, S-nitroso-N-acetylcysteine, S-nitrosoalbumin) present in the aqueous test solution. Sensitivity toward the different RSNO species is dependent on the respective catalytic rates of decomposition of the RSNO species by reactive Cu(I), accessibility of the species into the polyurethane layer containing the catalyst, the level of reducing agents (ascorbate) used in solution to help generate reactive Cu(I) species, and the concentration of metal ion complexing agents present in the test solution (e.g., EDTA). Under optimized conditions, all RSNO species can be detected at < or =1 microM levels, with sensor lifetimes of at least 10 days for the sensors based on Cu(II)-phosphate and Cu0 particles. It is further shown that the new RSNO sensors can be used to assess the "NO-generating" ability of fresh blood samples by effectively detecting the total level of reactive RSNO species present in such samples.

Animals↗

PubMed2004

Improved planar amperometric nitric oxide sensor based on platinized platinum anode. 1. Experimental results and theory when applied for monitoring NO release from diazeniumdiolate-doped polymeric films.

An improved miniature amperometric nitric oxide sensor design with a planar sensing tip (ranging from 150 microm to 2 mm in diameter) is reported. The sensor is fabricated using a platinized platinum anode and a Ag/AgCl cathode housed behind a microporous poly(tetrafluoroethylene) (PTFE; Gore-tex) gas-permeable membrane. Platinization of the working platinum electrode surface dramatically improves the analytical performance of the sensor by providing approximately 10-fold higher sensitivity (0.8-1.3 pA/nM), approximately 10-fold lower detection limit (< or =1 nM), and extended (at least 3-fold) stability (>3 d) compared to sensors prepared with bare Pt electrodes. These improvements in performance arise from increasing the kinetics and lowering the required potential for the 3-electron oxidation of NO to nitrate, relative to that observed using a nonplatinized working electrode. The outer porous PTFE membrane provides complete selectivity for NO over nitrite ions (up to 10 mM nitrite). The new sensor is applied for surface measurements of NO released from diazeniumdiolate-loaded silicone rubber films (SR-DACA-6/N(2)O(2)). The effects of sensor size (for sensor dimensions of 0.15-, 1-, and 2-mm o.d.) and the distance of the sensor from the surface of the NO-emitting polymer film are investigated via experiments as well as theoretical calculations. A significant analyte trapping effect is demonstrated, the degree of which depends on the sensor size and its distance from the surface. It is further demonstrated that surface NO concentrations for fresh SR-DACA-6/N(2)O(2) loaded films are also influenced by the polymer film thickness, with thicker films generating higher surface concentrations of NO.

Azo Compounds↗

PubMed2004

Catalytic generation of nitric oxide from nitrite at the interface of polymeric films doped with lipophilic CuII-complex: a potential route to the preparation of thromboresistant coatings.

A novel approach potentially useful for the development of more thromboresistant polymeric materials is examined. The method is based on the catalytic generation of nitric oxide (NO) via Cu(I) mediated reduction of nitrite ions. Preliminary solution phase studies demonstrate that ascorbate or thiolate anions can generate Cu(I) from Cu(II) with subsequent catalytic conversion of any nitrite ions present to NO by the unstable Cu(I) species. Incorporation of this same chemistry within a hydrophobic polymeric material requires immobilizing Cu(II) ions into a polymeric phase via use of a lipophilic Cu(II) chelating ligand (dibenzo [e,k]-2,3,8,9-tetraphenyl-1,4,7,10-tetraaza-cyclododeca-1,3,7,9-tetraene (DTTCT)). It is shown that this complex can be reduced to its Cu(I) form by appropriate reducing equivalents present in the bathing solution. The resulting Cu(I) complex can then reduce nitrite to NO with the NO generation occurring at the polymer/solution interface at physiological pH. Data from chemiluminescence experiments indicate that the flux of NO at the polymer surface is comparable to that of endothelial cells (>/=1x10(-10)mol/cm(2)min) when 0.5mM nitrite/1mM ascorbate are present in the bathing solution. Potentially more useful NO generation can be achieved by doping the polymer film with the Cu(II) complex along with a lipophilic quaternary ammonium nitrite salt. In this case reducing equivalents within the aqueous phase enable the nitrite derived from the polymer to be converted into NO by the Cu(II/I) ligand complex. Films of this type are shown to generate NO for at least 6h in PBS buffer with fluxes on the order of 1.5x10(-10)mol/cm(2)min. Physiologically relevant levels of NO release are also shown to exist at the polymer interface when films are soaked in fresh plasma as well as undiluted whole blood, indicating that endogenous reducing equivalents present in blood can efficiently reduce the Cu(II)-ligand within the polymer film. The prospects of using these new NO releasing films to devise more biocompatible polymeric coatings for biomedical applications are discussed.

Ascorbic Acid↗

PubMed2003

Spontaneous catalytic generation of nitric oxide from S-nitrosothiols at the surface of polymer films doped with lipophilic copperII complex.

A new approach for preparing potentially more blood-compatible nitric oxide (NO)-generating polymeric materials is described. The method involves creating polymeric films that have catalytic sites within (lipophilic copper(II) complex) that are capable of converting endogenous S-nitrosothiols present in blood (S-nitrosoglutathione (GSNO), S-nitrosocysteine (CysNO), etc.) to NO. The catalytic NO generation reaction involves the initial reduction of Cu(II) to Cu(I) within the complex by appropriate reducing agents (e.g., thiolates or ascorbate), followed by the reduction of S-nitrosothiols to NO by the Cu(I) complex at the polymer/solution interface. The NO fluxes observed when PVC or polyurethane films containing the copper(II) complex are placed in solutions containing physiological levels of nitrosothiols (muM levels) reach ca. 8 x 10-10 mol cm-2 min-1, greater than that produced by normal endothelial cells that line all healthy blood vessels. It is thus anticipated that this spontaneous catalytic generation of NO from endogenous nitrosothiols will render such polymeric materials more thromboresistant when in contact with blood in vivo.

Catalysis↗