Biomedical subjects

Brian S Anderson

Publications and source records attributed to Brian S Anderson.

7 recordsLinked to original sources

PubMed2006

Spatial relationships between water quality and pesticide application rates in agricultural watersheds.

Pesticide applications to agricultural lands in California, USA, are reported to a central data base, while data on water and sediment quality are collected by a number of monitoring programs. Data from both sources are geo-referenced, allowing spatial analysis of relationships between pesticide application rates and the chemical and biological condition of water bodies. This study collected data from 12 watersheds, selected to represent a range of pesticide usage. Water quality parameters were measured during six surveys of stream sites receiving runoff from the selected watershed areas. This study had three objectives: to evaluate the usefulness of pesticide application data in selecting regional monitoring sites, to provide information for generating and testing hypotheses about pesticide fate and effects, and to determine whether in-stream nitrate concentration was a useful surrogate indicator for regional monitoring of toxic substances. Significant correlations were observed between pesticide application rates and in-stream pesticide concentrations (p < 0.05) and toxicity (p < 0.10). In-stream nitrate concentrations were not significantly correlated with either the amount of pesticides applied, in-stream pesticide concentrations, or in-stream toxicity (all p > 0.30). Neither total watershed area nor the area in which pesticide usage was reported correlated significantly with the amount of pesticides applied, in-stream pesticide concentrations, or in-stream toxicity (all p > 0.14). In-stream pesticide concentrations and effects were more closely related to the intensity of pesticide use than to the area under cultivation.

Agriculture↗

PubMed2006

Evidence of pesticide impacts in the Santa Maria River watershed, California, USA.

The Santa Maria River provides significant freshwater and coastal habitat in a semiarid region of central California, USA. We conducted a water and sediment quality assessment consisting of chemical analyses, toxicity tests, toxicity identification evaluations, and macroinvertebrate bioassessments of samples from six stations collected during four surveys conducted between July 2002 and May 2003. Santa Maria River water samples collected downstream of Orcutt Creek (Santa Maria, Santa Barbara County, CA, USA), which conveys agriculture drain water, were acutely toxic to cladocera (Ceriodaphnia dubia), as were samples from Orcutt Creek. Toxicity identification evaluations (TIEs) suggested that toxicity to C. dubia in Orcutt Creek and the Santa Maria River was due to chlorpyrifos. Sediments from these two stations also were acutely toxic to the amphipod Hyalella azteca, a resident invertebrate. The TIEs conducted on sediment suggested that toxicity to amphipods, in part, was due to organophosphate pesticides. Concentrations of chlorpyrifos in pore water sometimes exceeded the 10-d median lethal concentration for H. azteca. Additional TIE and chemical evidence suggested sediment toxicity also partly could be due to pyrethroid pesticides. Relative to an upstream reference station, macroinvertebrate community structure was impacted in Orcutt Creek and in the Santa Maria River downstream of the Creek input. This study suggests that pesticide pollution likely is the cause of ecological damage in the Santa Maria River.

Amphipoda↗

PubMed2006

Solid-phase sediment toxicity identification evaluation in an agricultural stream.

The lower Santa Maria River watershed provides important aquatic habitat on the central California coast and is influenced heavily by agricultural runoff. As part of a recently completed water quality assessment, we conducted a series of water column and sediment toxicity tests throughout this watershed. Sediment from Orcutt Creek, a tributary that drains agricultural land, consistently was toxic to the amphipod Hyalella azteca, which is a resident genus in this river. Toxicity identification evaluations (TIEs) were conducted to determine cause(s) of toxicity. We observed no toxicity in sediment interstitial water even though concentrations of chlorpyrifos exceeded published aqueous toxicity thresholds for H. azteca. In contrast to interstitial water, bulk sediment was toxic to H. azteca. In bulk-phase sediment TIEs, the addition of 20% (by volume) coconut charcoal increased survival by 41%, implicating organic chemical(s). Addition of 5% (by volume) of the carbonaceous resin Ambersorb 563 increased survival by 88%, again suggesting toxicity due to organic chemicals. Toxicity was confirmed by isolating Ambersorb from the sediment, eluting the resin with methanol, and observing significant toxicity in control water spiked with the methanol eluate. A carboxylesterase enzyme that hydrolyzes synthetic pyrethroids was added to overlying water, and this significantly reduced toxicity to amphipods. Although the pesticides chlorpyrifos, DDT, permethrin, esfenvalerate, and fenvalerate were detected in this sediment, and their concentrations were below published toxicity thresholds for H. azteca, additivity or synergism may have occurred. The weight-of-evidence suggests toxicity of this sediment was caused by an organic contaminant, most likely a synthetic pyrethroid.

Agriculture↗

PubMed2004

In situ water and sediment toxicity in an agricultural watershed.

The Salinas River receives inputs from extensive farmlands before flowing into the Salinas River National Wildlife Refuge and the Monterey Bay National Marine Sanctuary (CA, USA). Previous monitoring using laboratory toxicity tests and chemical analyses identified toxic agricultural drain-water inputs in this system. Using caged daphnids (Ceriodaphnia dubia) and amphipods (Hyalella azteca), we investigated in situ toxicity at stations downstream from an agricultural drain relative to a reference station. A flow sensor indicated highly variable inputs from irrigation, and daily synoptic chemical analyses using enzyme-linked immunosorbent assay techniques demonstrated fluctuating concentrations of organophosphate pesticides. Test organism mortality in the field coincided with contaminant concentrations that exceeded chemical effect thresholds for the test species. Laboratory toxicity tests using C. dubia were comparable to results from field exposures, but tests with H. azteca were not. Laboratory exposures can be reasonable surrogates for field evaluations in this system, but they were less effective for assessing short-term temporal variability. Results from the field toxicity studies corroborated results of bioassessment surveys conducted as part of a concurrent study. Toxicity identification evaluations indicated that organophosphate pesticides caused toxicity to daphnids and that effects of suspended solids were negligible.

Agriculture↗

PubMed2003

Ambient toxicity due to chlorpyrifos and diazinon in a central California coastal watershed.

The Salinas River watershed along the central coast of California, U.S.A., supports rapidly growing urban areas and intensive agricultural operations. The river drains to an estuarine National Wildlife Refuge and a National Marine Sanctuary. The occurrence, spatial patterns, sources and causes of aquatic toxicity in the watershed were investigated by sampling four sites in the main river and four sites in representative tributaries during 15 surveys between September 1998 and January 2000. In 96 hr toxicity tests, significant Ceriodaphnia dubia mortality was observed in 11% of the main river samples, 87% of the samples from a channel draining an urban/agricultural watershed, 13% of the samples from channels conveying agricultural tile drain runoff, and in 100% of the samples from a channel conveying agricultural surface furrow runoff. In six of nine toxicity identification evaluations (TIEs), the organophosphate pesticides diazinon and/or chlorpyrifos were implicated as causes of observed toxicity, and these compounds were the most probable causes of toxicity in two of the other three TIEs. Every sample collected in the watershed that exhibited greater than 50% C. dubia mortality (n = 31) had sufficient diazinon and/or chlorpyrifos concentrations to account for the observed effects. Results are interpreted with respect to potential effects on other ecologically important species.

Agriculture↗

PubMed2003

Ecotoxicologic impacts of agricultural drain water in the Salinas River, California, USA.

The Salinas River is the largest of the three rivers that drain into the Monterey Bay National Marine Sanctuary in central California (USA). Large areas of this watershed are cultivated year-round in row crops, and previous laboratory studies have demonstrated that acute toxicity of agricultural drain water to Ceriodaphnia dubia is caused by the organophosphate (OP) pesticides chlorpyrifos and diazinon. We investigated chemical contamination and toxicity in waters and sediments in the river downstream of an agricultural drain water input. Ecological impacts of drain water were investigated by using bioassessments of macroinvertebrate community structure. Toxicity identification evaluations were used to characterize chemicals responsible for toxicity. Salinas River water downstream of the agricultural drain was acutely toxic to the cladoceran Ceriodaphnia dubia, and toxicity to C. dubia was highly correlated with combined toxic units (TUs) of chlorpyrifos and diazinon. Laboratory tests were used to demonstrate that sediments in this system were acutely toxic to the amphipod Hyalella azteca, a resident invertebrate. Toxicity identification evaluations (TIEs) conducted on sediment pore water suggested that toxicity to amphipods was due in part to OP pesticides; concentrations of chlorpyrifos in pore water sometimes exceeded the 10-d mean lethal concentration (LC50) for H. azteca. Potentiation of toxicity with addition of the metabolic inhibitor piperonyl butoxide suggested that sediment toxicity also was due to other non-metabolically activated compounds. Macroinvertebrate community structure was highly impacted downstream of the agricultural drain input, and a number of macroinvertebrate community metrics were negatively correlated with combined TUs of chlorpyrifos and diazinon, as well as turbidity associated with the drain water. Some macroinvertebrate metrics were also correlated with bank vegetation cover. This study suggests that pesticide pollution is the likely cause of ecological damage in the Salinas River, and this factor may interact with other stressors associated with agricultural drain water to impact the macroinvertebrate community in the system.

Agriculture↗

PubMed2002

Acutei and chronic toxicity of nickel to marine organisms: implications for water quality criteria.

Acute and chronic toxicity tests were conducted to determine the effects of nickel on three U.S. west coast marine species: a fish (the topsmelt, Atherinops affinis), a mollusk (the red abalone, Haliotis rufescens), and a crustacean (the mysid, Mysidopsis intii). The 96-h median lethal concentration (LC50) for topsmelt was 26,560 microg/L, and the chronic value for the most sensitive endpoint in a 40-d exposure was 4,270 microg/L. The median effective concentration (EC50) for 48-h abalone larval development was 145.5 microg/L, and the chronic value for juvenile growth in a 22-d exposure through larval metamorphosis was 26.43 microgAL. The mysid 96-h LC50 was 148.6 microg/L, and the chronic value for the most sensitive endpoint in a 28-d, whole life-cycle exposure was 22.09 microg/L. The abalone and mysid acute values were lower than other values available in the literature. Acute-to-chronic ratios for nickel toxicity to the three species were 6.220, 5.505, and 6.727, respectively, which were similar to the only other available saltwater value of 5.478 (for Americamysis [Mysidopsis] bahia) and significantly lower than the existing values of 35.58 and 29.86 for freshwater organisms. Incorporation of data from the present study into calculations for water quality criteria would lower the criterion maximum concentration and raise the criterion continuous concentration for nickel.

Animals↗